Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1652 and Shewana3_1653 are separated by 112 nucleotides Shewana3_1653 and bcp are separated by 180 nucleotides bcp and Shewana3_1655 are separated by 3 nucleotides Shewana3_1655 and Shewana3_1656 are separated by 172 nucleotides
Shewana3_1652: Shewana3_1652 - chromosome replication initiation inhibitor protein (RefSeq), at 1,943,259 to 1,944,176
_1652
Shewana3_1653: Shewana3_1653 - lysine exporter protein LysE/YggA (RefSeq), at 1,944,289 to 1,944,903
_1653
Shewana3_1654: bcp - thioredoxin-dependent thiol peroxidase (RefSeq), at 1,945,084 to 1,945,551
bcp
Shewana3_1655: Shewana3_1655 - glycine cleavage system transcriptional repressor, putative (RefSeq), at 1,945,555 to 1,946,106
_1655
Shewana3_1656: Shewana3_1656 - dihydrodipicolinate synthase (RefSeq), at 1,946,279 to 1,947,163
_1656
Position (kb)
1945
1946 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1944.387 kb on + strand, within Shewana3_1653 at 1944.395 kb on - strand, within Shewana3_1653 at 1944.466 kb on - strand, within Shewana3_1653 at 1944.480 kb on + strand, within Shewana3_1653 at 1944.541 kb on - strand, within Shewana3_1653 at 1944.638 kb on + strand, within Shewana3_1653 at 1945.135 kb on - strand, within bcp at 1945.278 kb on + strand, within bcp at 1945.399 kb on + strand, within bcp at 1945.399 kb on + strand, within bcp at 1945.407 kb on - strand, within bcp at 1945.417 kb on - strand, within bcp at 1945.417 kb on - strand, within bcp at 1945.704 kb on - strand, within Shewana3_1655 at 1945.777 kb on - strand, within Shewana3_1655 at 1945.788 kb on + strand, within Shewana3_1655 at 1945.868 kb on - strand, within Shewana3_1655 at 1945.960 kb on - strand, within Shewana3_1655 at 1946.143 kb on + strand at 1946.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,944,387 + Shewana3_1653 0.16 +0.5 1,944,395 - Shewana3_1653 0.17 +3.0 1,944,466 - Shewana3_1653 0.29 +3.0 1,944,480 + Shewana3_1653 0.31 +1.7 1,944,541 - Shewana3_1653 0.41 +1.1 1,944,638 + Shewana3_1653 0.57 +0.2 1,945,135 - bcp Shewana3_1654 0.11 +0.7 1,945,278 + bcp Shewana3_1654 0.41 -0.7 1,945,399 + bcp Shewana3_1654 0.67 +0.3 1,945,399 + bcp Shewana3_1654 0.67 +4.0 1,945,407 - bcp Shewana3_1654 0.69 -0.1 1,945,417 - bcp Shewana3_1654 0.71 -0.1 1,945,417 - bcp Shewana3_1654 0.71 -0.3 1,945,704 - Shewana3_1655 0.27 -1.0 1,945,777 - Shewana3_1655 0.40 +0.3 1,945,788 + Shewana3_1655 0.42 +0.5 1,945,868 - Shewana3_1655 0.57 -0.4 1,945,960 - Shewana3_1655 0.73 +0.8 1,946,143 + +0.7 1,946,174 + +2.0
Or see this region's nucleotide sequence