Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1422 and Shewana3_1423 are separated by 65 nucleotides Shewana3_1423 and Shewana3_1424 are separated by 34 nucleotides Shewana3_1424 and Shewana3_1425 are separated by 91 nucleotides
Shewana3_1422: Shewana3_1422 - 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq), at 1,651,742 to 1,653,025
_1422
Shewana3_1423: Shewana3_1423 - Dual specificity protein phosphatase (RefSeq), at 1,653,091 to 1,653,561
_1423
Shewana3_1424: Shewana3_1424 - phospholipase D/transphosphatidylase (RefSeq), at 1,653,596 to 1,655,230
_1424
Shewana3_1425: Shewana3_1425 - response regulator receiver modulated CheW protein (RefSeq), at 1,655,322 to 1,656,221
_1425
Position (kb)
1653
1654
1655
1656 Strain fitness (log2 ratio)
-1
0
1
2 at 1653.177 kb on - strand, within Shewana3_1423 at 1653.191 kb on + strand, within Shewana3_1423 at 1653.357 kb on - strand, within Shewana3_1423 at 1653.400 kb on + strand, within Shewana3_1423 at 1653.817 kb on - strand, within Shewana3_1424 at 1654.067 kb on + strand, within Shewana3_1424 at 1654.123 kb on + strand, within Shewana3_1424 at 1654.183 kb on + strand, within Shewana3_1424 at 1654.446 kb on - strand, within Shewana3_1424 at 1654.868 kb on - strand, within Shewana3_1424 at 1655.078 kb on + strand at 1655.224 kb on - strand at 1655.229 kb on + strand at 1655.332 kb on + strand at 1655.381 kb on + strand at 1655.381 kb on + strand at 1655.420 kb on + strand, within Shewana3_1425 at 1655.428 kb on - strand, within Shewana3_1425 at 1655.542 kb on + strand, within Shewana3_1425 at 1655.550 kb on - strand, within Shewana3_1425
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,653,177 - Shewana3_1423 0.18 -0.1 1,653,191 + Shewana3_1423 0.21 -0.5 1,653,357 - Shewana3_1423 0.56 +2.2 1,653,400 + Shewana3_1423 0.66 +0.6 1,653,817 - Shewana3_1424 0.14 +1.2 1,654,067 + Shewana3_1424 0.29 -0.8 1,654,123 + Shewana3_1424 0.32 +0.6 1,654,183 + Shewana3_1424 0.36 +0.8 1,654,446 - Shewana3_1424 0.52 +1.0 1,654,868 - Shewana3_1424 0.78 +0.5 1,655,078 + +1.6 1,655,224 - -0.1 1,655,229 + -0.5 1,655,332 + +1.2 1,655,381 + -0.6 1,655,381 + -0.6 1,655,420 + Shewana3_1425 0.11 -1.1 1,655,428 - Shewana3_1425 0.12 +2.2 1,655,542 + Shewana3_1425 0.24 +2.2 1,655,550 - Shewana3_1425 0.25 +2.2
Or see this region's nucleotide sequence