Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1420 and Shewana3_1421 are separated by 128 nucleotides Shewana3_1421 and Shewana3_1422 are separated by 37 nucleotides
Shewana3_1420: Shewana3_1420 - transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II (RefSeq), at 1,649,381 to 1,651,009
_1420
Shewana3_1421: Shewana3_1421 - methylated-DNA--protein-cysteine methyltransferase (RefSeq), at 1,651,138 to 1,651,704
_1421
Shewana3_1422: Shewana3_1422 - 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq), at 1,651,742 to 1,653,025
_1422
Position (kb)
1651
1652 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1650.284 kb on + strand, within Shewana3_1420 at 1650.284 kb on + strand, within Shewana3_1420 at 1650.292 kb on - strand, within Shewana3_1420 at 1650.342 kb on + strand, within Shewana3_1420 at 1650.442 kb on + strand, within Shewana3_1420 at 1650.641 kb on + strand, within Shewana3_1420 at 1651.132 kb on + strand at 1651.146 kb on + strand at 1651.335 kb on + strand, within Shewana3_1421 at 1651.335 kb on + strand, within Shewana3_1421 at 1651.412 kb on + strand, within Shewana3_1421 at 1651.529 kb on - strand, within Shewana3_1421 at 1651.825 kb on - strand at 1652.577 kb on + strand, within Shewana3_1422
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,650,284 + Shewana3_1420 0.55 -1.9 1,650,284 + Shewana3_1420 0.55 -0.4 1,650,292 - Shewana3_1420 0.56 +1.8 1,650,342 + Shewana3_1420 0.59 -0.8 1,650,442 + Shewana3_1420 0.65 +0.1 1,650,641 + Shewana3_1420 0.77 -1.7 1,651,132 + +1.5 1,651,146 + +0.3 1,651,335 + Shewana3_1421 0.35 -1.1 1,651,335 + Shewana3_1421 0.35 -0.8 1,651,412 + Shewana3_1421 0.48 +0.1 1,651,529 - Shewana3_1421 0.69 +0.8 1,651,825 - +1.8 1,652,577 + Shewana3_1422 0.65 +2.0
Or see this region's nucleotide sequence