Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhF and Shewana3_1358 are separated by 4 nucleotides Shewana3_1358 and fliA overlap by 8 nucleotides fliA and Shewana3_1360 are separated by 47 nucleotides Shewana3_1360 and Shewana3_1361 are separated by 13 nucleotides
Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345
flhF
Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231
_1358
Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937
fliA
Shewana3_1360: Shewana3_1360 - response regulator receiver protein (RefSeq), at 1,572,985 to 1,573,368
_1360
Shewana3_1361: Shewana3_1361 - chemotaxis phosphatase, CheZ (RefSeq), at 1,573,382 to 1,574,119
_1361
Position (kb)
1572
1573 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1571.587 kb on + strand, within Shewana3_1358 at 1571.602 kb on + strand, within Shewana3_1358 at 1571.812 kb on + strand, within Shewana3_1358 at 1572.075 kb on + strand, within Shewana3_1358 at 1572.239 kb on + strand at 1572.288 kb on - strand at 1572.376 kb on + strand, within fliA at 1572.524 kb on + strand, within fliA at 1572.806 kb on + strand, within fliA at 1572.907 kb on + strand at 1573.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,571,587 + Shewana3_1358 0.27 +0.3 1,571,602 + Shewana3_1358 0.29 +0.2 1,571,812 + Shewana3_1358 0.52 -1.9 1,572,075 + Shewana3_1358 0.82 +0.2 1,572,239 + +5.7 1,572,288 - +1.7 1,572,376 + fliA Shewana3_1359 0.21 +0.0 1,572,524 + fliA Shewana3_1359 0.42 +0.8 1,572,806 + fliA Shewana3_1359 0.82 +1.5 1,572,907 + -1.5 1,573,403 - +1.7
Or see this region's nucleotide sequence