Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1181 and trmB are separated by 179 nucleotides trmB and Shewana3_1183 are separated by 45 nucleotides Shewana3_1183 and Shewana3_1184 overlap by 1 nucleotides
Shewana3_1181: Shewana3_1181 - A/G-specific DNA-adenine glycosylase (RefSeq), at 1,388,541 to 1,389,659
_1181
Shewana3_1182: trmB - tRNA (guanine-N(7)-)-methyltransferase (RefSeq), at 1,389,839 to 1,390,555
trmB
Shewana3_1183: Shewana3_1183 - hypothetical protein (RefSeq), at 1,390,601 to 1,390,927
_1183
Shewana3_1184: Shewana3_1184 - Glutaminase (EC 3.5.1.2) (from data) , at 1,390,927 to 1,391,841
_1184
Position (kb)
1389
1390
1391 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1388.980 kb on + strand, within Shewana3_1181 at 1389.147 kb on - strand, within Shewana3_1181 at 1389.276 kb on + strand, within Shewana3_1181 at 1389.281 kb on + strand, within Shewana3_1181 at 1389.317 kb on + strand, within Shewana3_1181 at 1389.382 kb on + strand, within Shewana3_1181 at 1389.473 kb on - strand, within Shewana3_1181 at 1389.960 kb on + strand, within trmB at 1390.008 kb on + strand, within trmB at 1391.340 kb on + strand, within Shewana3_1184 at 1391.348 kb on - strand, within Shewana3_1184 at 1391.401 kb on + strand, within Shewana3_1184 at 1391.409 kb on + strand, within Shewana3_1184 at 1391.482 kb on - strand, within Shewana3_1184 at 1391.547 kb on + strand, within Shewana3_1184
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,388,980 + Shewana3_1181 0.39 -2.1 1,389,147 - Shewana3_1181 0.54 -0.9 1,389,276 + Shewana3_1181 0.66 +1.5 1,389,281 + Shewana3_1181 0.66 -0.6 1,389,317 + Shewana3_1181 0.69 +0.5 1,389,382 + Shewana3_1181 0.75 -1.4 1,389,473 - Shewana3_1181 0.83 -0.2 1,389,960 + trmB Shewana3_1182 0.17 +3.4 1,390,008 + trmB Shewana3_1182 0.24 -0.5 1,391,340 + Shewana3_1184 0.45 -0.7 1,391,348 - Shewana3_1184 0.46 +1.0 1,391,401 + Shewana3_1184 0.52 -1.8 1,391,409 + Shewana3_1184 0.53 +0.4 1,391,482 - Shewana3_1184 0.61 +3.5 1,391,547 + Shewana3_1184 0.68 +0.3
Or see this region's nucleotide sequence