Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0517 and Shewana3_0518 are separated by 77 nucleotides Shewana3_0518 and Shewana3_0519 are separated by 129 nucleotides Shewana3_0519 and mscL are separated by 82 nucleotides
Shewana3_0517: Shewana3_0517 - RND family efflux transporter MFP subunit (RefSeq), at 606,137 to 607,417
_0517
Shewana3_0518: Shewana3_0518 - CzcA family heavy metal efflux protein (RefSeq), at 607,495 to 610,707
_0518
Shewana3_0519: Shewana3_0519 - antibiotic biosynthesis monooxygenase (RefSeq), at 610,837 to 611,160
_0519
Shewana3_0520: mscL - large-conductance mechanosensitive channel (RefSeq), at 611,243 to 611,653
mscL
Position (kb) 607
608
609
610
611 Strain fitness (log2 ratio) -2
-1
0
1 at 606.661 kb on + strand, within Shewana3_0517 at 606.727 kb on - strand, within Shewana3_0517 at 606.884 kb on + strand, within Shewana3_0517 at 606.913 kb on + strand, within Shewana3_0517 at 607.031 kb on + strand, within Shewana3_0517 at 607.075 kb on + strand, within Shewana3_0517 at 607.101 kb on - strand, within Shewana3_0517 at 607.144 kb on - strand, within Shewana3_0517 at 607.277 kb on - strand, within Shewana3_0517 at 607.332 kb on - strand at 607.384 kb on + strand at 607.384 kb on + strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.640 kb on + strand at 607.659 kb on - strand at 607.711 kb on + strand at 607.745 kb on + strand at 607.783 kb on - strand at 607.865 kb on + strand, within Shewana3_0518 at 607.895 kb on + strand, within Shewana3_0518 at 608.001 kb on + strand, within Shewana3_0518 at 608.091 kb on + strand, within Shewana3_0518 at 608.230 kb on - strand, within Shewana3_0518 at 608.268 kb on + strand, within Shewana3_0518 at 608.291 kb on + strand, within Shewana3_0518 at 608.301 kb on + strand, within Shewana3_0518 at 608.481 kb on + strand, within Shewana3_0518 at 608.481 kb on + strand, within Shewana3_0518 at 608.481 kb on + strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.489 kb on - strand, within Shewana3_0518 at 608.543 kb on - strand, within Shewana3_0518 at 608.562 kb on + strand, within Shewana3_0518 at 608.637 kb on + strand, within Shewana3_0518 at 608.637 kb on + strand, within Shewana3_0518 at 608.640 kb on + strand, within Shewana3_0518 at 608.670 kb on + strand, within Shewana3_0518 at 608.728 kb on - strand, within Shewana3_0518 at 608.753 kb on + strand, within Shewana3_0518 at 608.868 kb on + strand, within Shewana3_0518 at 608.885 kb on + strand, within Shewana3_0518 at 608.901 kb on + strand, within Shewana3_0518 at 609.024 kb on + strand, within Shewana3_0518 at 609.157 kb on + strand, within Shewana3_0518 at 609.165 kb on - strand, within Shewana3_0518 at 609.196 kb on - strand, within Shewana3_0518 at 609.211 kb on - strand, within Shewana3_0518 at 609.229 kb on + strand, within Shewana3_0518 at 609.290 kb on + strand, within Shewana3_0518 at 609.290 kb on + strand, within Shewana3_0518 at 609.390 kb on + strand, within Shewana3_0518 at 609.457 kb on - strand, within Shewana3_0518 at 609.629 kb on + strand, within Shewana3_0518 at 609.761 kb on - strand, within Shewana3_0518 at 609.928 kb on + strand, within Shewana3_0518 at 609.938 kb on + strand, within Shewana3_0518 at 610.010 kb on + strand, within Shewana3_0518 at 610.054 kb on + strand, within Shewana3_0518 at 610.062 kb on - strand, within Shewana3_0518 at 610.085 kb on - strand, within Shewana3_0518 at 610.163 kb on + strand, within Shewana3_0518 at 610.233 kb on - strand, within Shewana3_0518 at 610.268 kb on + strand, within Shewana3_0518 at 610.439 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.560 kb on - strand at 610.568 kb on + strand at 610.574 kb on - strand at 610.632 kb on - strand at 610.639 kb on + strand at 610.647 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.743 kb on + strand at 610.930 kb on + strand, within Shewana3_0519 at 610.984 kb on + strand, within Shewana3_0519 at 611.031 kb on - strand, within Shewana3_0519 at 611.119 kb on + strand, within Shewana3_0519 at 611.127 kb on - strand, within Shewana3_0519 at 611.140 kb on + strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.464 kb on - strand, within mscL at 611.512 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 606,661 + Shewana3_0517 0.41 -0.6 606,727 - Shewana3_0517 0.46 +0.2 606,884 + Shewana3_0517 0.58 +0.1 606,913 + Shewana3_0517 0.61 -0.1 607,031 + Shewana3_0517 0.70 -1.1 607,075 + Shewana3_0517 0.73 -1.3 607,101 - Shewana3_0517 0.75 +0.2 607,144 - Shewana3_0517 0.79 +0.2 607,277 - Shewana3_0517 0.89 -0.0 607,332 - -1.7 607,384 + +1.0 607,384 + -0.6 607,392 - -0.7 607,392 - -2.5 607,392 - -0.4 607,392 - -0.4 607,640 + -0.6 607,659 - -1.9 607,711 + -0.4 607,745 + -0.4 607,783 - -1.3 607,865 + Shewana3_0518 0.12 -0.2 607,895 + Shewana3_0518 0.12 +0.8 608,001 + Shewana3_0518 0.16 -1.1 608,091 + Shewana3_0518 0.19 +0.4 608,230 - Shewana3_0518 0.23 -0.5 608,268 + Shewana3_0518 0.24 +0.2 608,291 + Shewana3_0518 0.25 -2.2 608,301 + Shewana3_0518 0.25 -0.2 608,481 + Shewana3_0518 0.31 +0.1 608,481 + Shewana3_0518 0.31 -0.4 608,481 + Shewana3_0518 0.31 -0.1 608,489 - Shewana3_0518 0.31 -1.3 608,489 - Shewana3_0518 0.31 +1.0 608,489 - Shewana3_0518 0.31 +0.4 608,489 - Shewana3_0518 0.31 -0.5 608,489 - Shewana3_0518 0.31 -1.9 608,489 - Shewana3_0518 0.31 -1.6 608,543 - Shewana3_0518 0.33 -1.5 608,562 + Shewana3_0518 0.33 +0.2 608,637 + Shewana3_0518 0.36 +1.4 608,637 + Shewana3_0518 0.36 -1.0 608,640 + Shewana3_0518 0.36 -0.2 608,670 + Shewana3_0518 0.37 -0.6 608,728 - Shewana3_0518 0.38 +1.0 608,753 + Shewana3_0518 0.39 +0.1 608,868 + Shewana3_0518 0.43 -0.2 608,885 + Shewana3_0518 0.43 -0.8 608,901 + Shewana3_0518 0.44 -0.0 609,024 + Shewana3_0518 0.48 -0.9 609,157 + Shewana3_0518 0.52 -1.3 609,165 - Shewana3_0518 0.52 +0.7 609,196 - Shewana3_0518 0.53 -1.5 609,211 - Shewana3_0518 0.53 -1.7 609,229 + Shewana3_0518 0.54 -0.2 609,290 + Shewana3_0518 0.56 -0.3 609,290 + Shewana3_0518 0.56 -0.1 609,390 + Shewana3_0518 0.59 -0.6 609,457 - Shewana3_0518 0.61 -0.9 609,629 + Shewana3_0518 0.66 -0.0 609,761 - Shewana3_0518 0.71 +0.5 609,928 + Shewana3_0518 0.76 +1.1 609,938 + Shewana3_0518 0.76 -0.4 610,010 + Shewana3_0518 0.78 +0.1 610,054 + Shewana3_0518 0.80 -0.8 610,062 - Shewana3_0518 0.80 -1.2 610,085 - Shewana3_0518 0.81 +0.0 610,163 + Shewana3_0518 0.83 -0.1 610,233 - Shewana3_0518 0.85 +1.7 610,268 + Shewana3_0518 0.86 -0.1 610,439 + -0.8 610,528 + +0.1 610,528 + +0.0 610,528 + +0.4 610,560 - -1.8 610,568 + -2.0 610,574 - -0.7 610,632 - -0.7 610,639 + +0.6 610,647 - -0.5 610,710 - -1.3 610,710 - -1.3 610,710 - -1.0 610,743 + +1.2 610,930 + Shewana3_0519 0.29 +0.6 610,984 + Shewana3_0519 0.45 -0.2 611,031 - Shewana3_0519 0.60 -0.3 611,119 + Shewana3_0519 0.87 -0.2 611,127 - Shewana3_0519 0.90 +0.8 611,140 + -0.2 611,267 - +0.5 611,267 - +0.5 611,267 - +0.6 611,464 - mscL Shewana3_0520 0.54 -1.3 611,512 - mscL Shewana3_0520 0.65 -0.3 611,557 - mscL Shewana3_0520 0.76 -0.2 611,557 - mscL Shewana3_0520 0.76 -0.8 611,636 - -0.2
Or see this region's nucleotide sequence