Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0437 and Shewana3_0438 are separated by 3 nucleotides Shewana3_0438 and Shewana3_0439 are separated by 157 nucleotides Shewana3_0439 and Shewana3_0440 are separated by 180 nucleotides
Shewana3_0437: Shewana3_0437 - short chain dehydrogenase (RefSeq), at 509,792 to 510,595
_0437
Shewana3_0438: Shewana3_0438 - hypothetical protein (RefSeq), at 510,599 to 510,823
_0438
Shewana3_0439: Shewana3_0439 - hypothetical protein (RefSeq), at 510,981 to 514,202
_0439
Shewana3_0440: Shewana3_0440 - phosphoribosylamine--glycine ligase (RefSeq), at 514,383 to 515,681
_0440
Position (kb) 510
511
512
513
514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 509.991 kb on - strand, within Shewana3_0437 at 510.005 kb on + strand, within Shewana3_0437 at 510.039 kb on + strand, within Shewana3_0437 at 510.167 kb on + strand, within Shewana3_0437 at 510.175 kb on - strand, within Shewana3_0437 at 510.185 kb on - strand, within Shewana3_0437 at 510.202 kb on - strand, within Shewana3_0437 at 510.234 kb on - strand, within Shewana3_0437 at 510.399 kb on - strand, within Shewana3_0437 at 510.637 kb on - strand, within Shewana3_0438 at 510.733 kb on - strand, within Shewana3_0438 at 510.793 kb on + strand, within Shewana3_0438 at 510.849 kb on + strand at 510.857 kb on - strand at 510.881 kb on - strand at 510.948 kb on + strand at 510.953 kb on + strand at 510.979 kb on + strand at 511.059 kb on + strand at 511.104 kb on - strand at 511.122 kb on - strand at 511.230 kb on - strand at 511.320 kb on + strand, within Shewana3_0439 at 511.376 kb on + strand, within Shewana3_0439 at 511.649 kb on - strand, within Shewana3_0439 at 511.716 kb on - strand, within Shewana3_0439 at 511.764 kb on + strand, within Shewana3_0439 at 511.764 kb on + strand, within Shewana3_0439 at 512.003 kb on + strand, within Shewana3_0439 at 512.052 kb on - strand, within Shewana3_0439 at 512.113 kb on + strand, within Shewana3_0439 at 512.394 kb on + strand, within Shewana3_0439 at 512.441 kb on - strand, within Shewana3_0439 at 512.555 kb on - strand, within Shewana3_0439 at 512.564 kb on - strand, within Shewana3_0439 at 512.588 kb on + strand, within Shewana3_0439 at 512.593 kb on + strand, within Shewana3_0439 at 512.593 kb on + strand, within Shewana3_0439 at 512.593 kb on + strand, within Shewana3_0439 at 512.596 kb on - strand, within Shewana3_0439 at 512.635 kb on + strand, within Shewana3_0439 at 512.829 kb on + strand, within Shewana3_0439 at 513.222 kb on - strand, within Shewana3_0439 at 513.304 kb on - strand, within Shewana3_0439 at 513.378 kb on - strand, within Shewana3_0439 at 513.602 kb on + strand, within Shewana3_0439 at 513.737 kb on - strand, within Shewana3_0439 at 513.830 kb on + strand, within Shewana3_0439 at 513.838 kb on - strand, within Shewana3_0439 at 514.068 kb on - strand at 514.092 kb on + strand at 514.386 kb on + strand at 514.394 kb on - strand at 514.494 kb on - strand at 514.509 kb on + strand at 514.511 kb on - strand at 514.536 kb on + strand, within Shewana3_0440 at 514.552 kb on - strand, within Shewana3_0440 at 514.582 kb on + strand, within Shewana3_0440 at 514.613 kb on - strand, within Shewana3_0440 at 514.613 kb on - strand, within Shewana3_0440 at 514.713 kb on - strand, within Shewana3_0440 at 514.732 kb on + strand, within Shewana3_0440 at 514.845 kb on + strand, within Shewana3_0440 at 514.853 kb on - strand, within Shewana3_0440 at 514.919 kb on + strand, within Shewana3_0440 at 514.999 kb on - strand, within Shewana3_0440 at 515.103 kb on - strand, within Shewana3_0440
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 509,991 - Shewana3_0437 0.25 -2.6 510,005 + Shewana3_0437 0.26 -0.7 510,039 + Shewana3_0437 0.31 -0.4 510,167 + Shewana3_0437 0.47 +0.9 510,175 - Shewana3_0437 0.48 -1.9 510,185 - Shewana3_0437 0.49 -0.5 510,202 - Shewana3_0437 0.51 -0.7 510,234 - Shewana3_0437 0.55 -1.1 510,399 - Shewana3_0437 0.75 -0.8 510,637 - Shewana3_0438 0.17 -1.0 510,733 - Shewana3_0438 0.60 -0.6 510,793 + Shewana3_0438 0.86 -1.3 510,849 + -0.9 510,857 - -1.5 510,881 - -0.2 510,948 + -1.7 510,953 + -1.6 510,979 + -0.8 511,059 + +0.6 511,104 - +0.6 511,122 - -0.1 511,230 - +0.2 511,320 + Shewana3_0439 0.11 -1.1 511,376 + Shewana3_0439 0.12 +0.0 511,649 - Shewana3_0439 0.21 +0.9 511,716 - Shewana3_0439 0.23 -0.7 511,764 + Shewana3_0439 0.24 +0.7 511,764 + Shewana3_0439 0.24 -0.1 512,003 + Shewana3_0439 0.32 +0.3 512,052 - Shewana3_0439 0.33 -0.2 512,113 + Shewana3_0439 0.35 -3.0 512,394 + Shewana3_0439 0.44 -1.0 512,441 - Shewana3_0439 0.45 +0.3 512,555 - Shewana3_0439 0.49 -2.2 512,564 - Shewana3_0439 0.49 -0.5 512,588 + Shewana3_0439 0.50 -0.4 512,593 + Shewana3_0439 0.50 -1.0 512,593 + Shewana3_0439 0.50 -0.3 512,593 + Shewana3_0439 0.50 -0.2 512,596 - Shewana3_0439 0.50 -1.2 512,635 + Shewana3_0439 0.51 +0.4 512,829 + Shewana3_0439 0.57 -1.7 513,222 - Shewana3_0439 0.70 -0.3 513,304 - Shewana3_0439 0.72 +0.2 513,378 - Shewana3_0439 0.74 -0.8 513,602 + Shewana3_0439 0.81 -0.4 513,737 - Shewana3_0439 0.86 -0.1 513,830 + Shewana3_0439 0.88 +0.8 513,838 - Shewana3_0439 0.89 -2.3 514,068 - +0.1 514,092 + -0.6 514,386 + -1.1 514,394 - +0.1 514,494 - +0.5 514,509 + -1.2 514,511 - +0.2 514,536 + Shewana3_0440 0.12 +0.0 514,552 - Shewana3_0440 0.13 +0.0 514,582 + Shewana3_0440 0.15 -1.2 514,613 - Shewana3_0440 0.18 +0.0 514,613 - Shewana3_0440 0.18 -2.2 514,713 - Shewana3_0440 0.25 -0.1 514,732 + Shewana3_0440 0.27 -0.4 514,845 + Shewana3_0440 0.36 -1.6 514,853 - Shewana3_0440 0.36 -0.6 514,919 + Shewana3_0440 0.41 +0.1 514,999 - Shewana3_0440 0.47 -0.1 515,103 - Shewana3_0440 0.55 -0.4
Or see this region's nucleotide sequence