Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0424 and pdhR are separated by 325 nucleotides pdhR and aceE are separated by 80 nucleotides
Shewana3_0424: Shewana3_0424 - regulatory protein AmpE (RefSeq), at 483,246 to 484,097
_0424
Shewana3_0425: pdhR - transcriptional regulator PdhR (RefSeq), at 484,423 to 485,175
pdhR
Shewana3_0426: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 485,256 to 487,922
aceE
Position (kb) 484
485
486 Strain fitness (log2 ratio) -2
-1
0
1
2 at 483.732 kb on + strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.737 kb on + strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.860 kb on + strand, within Shewana3_0424 at 483.872 kb on + strand, within Shewana3_0424 at 483.876 kb on + strand, within Shewana3_0424 at 483.892 kb on - strand, within Shewana3_0424 at 483.960 kb on + strand, within Shewana3_0424 at 484.039 kb on + strand at 484.039 kb on + strand at 484.039 kb on + strand at 484.047 kb on - strand at 484.165 kb on + strand at 484.233 kb on + strand at 484.513 kb on + strand, within pdhR at 484.918 kb on + strand, within pdhR at 485.241 kb on + strand at 485.271 kb on + strand at 485.278 kb on + strand at 485.314 kb on - strand at 485.325 kb on + strand at 485.408 kb on + strand at 485.416 kb on - strand at 485.800 kb on - strand, within aceE at 485.882 kb on + strand, within aceE at 485.989 kb on - strand, within aceE at 486.036 kb on - strand, within aceE at 486.068 kb on + strand, within aceE at 486.068 kb on + strand, within aceE
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 483,732 + Shewana3_0424 0.57 -0.5 483,732 + Shewana3_0424 0.57 -0.1 483,737 + Shewana3_0424 0.58 -0.3 483,740 - Shewana3_0424 0.58 -0.5 483,740 - Shewana3_0424 0.58 -0.9 483,740 - Shewana3_0424 0.58 -0.7 483,745 - Shewana3_0424 0.59 +1.4 483,745 - Shewana3_0424 0.59 -0.9 483,745 - Shewana3_0424 0.59 -1.1 483,860 + Shewana3_0424 0.72 +0.8 483,872 + Shewana3_0424 0.73 +0.4 483,876 + Shewana3_0424 0.74 -0.8 483,892 - Shewana3_0424 0.76 -2.4 483,960 + Shewana3_0424 0.84 +0.1 484,039 + -0.5 484,039 + +0.4 484,039 + -0.3 484,047 - +0.1 484,165 + +0.0 484,233 + -0.5 484,513 + pdhR Shewana3_0425 0.12 +2.3 484,918 + pdhR Shewana3_0425 0.66 +0.7 485,241 + +1.5 485,271 + +1.0 485,278 + -0.6 485,314 - +0.5 485,325 + +0.8 485,408 + +1.1 485,416 - -0.1 485,800 - aceE Shewana3_0426 0.20 -1.2 485,882 + aceE Shewana3_0426 0.23 +2.0 485,989 - aceE Shewana3_0426 0.27 -0.1 486,036 - aceE Shewana3_0426 0.29 -0.0 486,068 + aceE Shewana3_0426 0.30 +0.9 486,068 + aceE Shewana3_0426 0.30 +1.2
Or see this region's nucleotide sequence