Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdB and Shewana3_0405 are separated by 63 nucleotides Shewana3_0405 and Shewana3_0406 overlap by 4 nucleotides Shewana3_0406 and Shewana3_0407 are separated by 118 nucleotides
Shewana3_0404: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 463,964 to 464,695
frdB
Shewana3_0405: Shewana3_0405 - antibiotic biosynthesis monooxygenase (RefSeq), at 464,759 to 465,043
_0405
Shewana3_0406: Shewana3_0406 - alcohol dehydrogenase (RefSeq), at 465,040 to 466,062
_0406
Shewana3_0407: Shewana3_0407 - LysR family transcriptional regulator (RefSeq), at 466,181 to 467,113
_0407
Position (kb) 465
466
467 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 464.103 kb on - strand, within frdB at 464.375 kb on - strand, within frdB at 464.452 kb on - strand, within frdB at 464.479 kb on - strand, within frdB at 464.539 kb on + strand, within frdB at 464.573 kb on + strand, within frdB at 464.578 kb on + strand, within frdB at 464.773 kb on - strand at 464.792 kb on + strand, within Shewana3_0405 at 464.800 kb on - strand, within Shewana3_0405 at 464.810 kb on - strand, within Shewana3_0405 at 464.905 kb on + strand, within Shewana3_0405 at 464.913 kb on + strand, within Shewana3_0405 at 464.937 kb on - strand, within Shewana3_0405 at 464.958 kb on + strand, within Shewana3_0405 at 464.966 kb on - strand, within Shewana3_0405 at 464.971 kb on + strand, within Shewana3_0405 at 464.979 kb on - strand, within Shewana3_0405 at 464.979 kb on - strand, within Shewana3_0405 at 464.991 kb on + strand, within Shewana3_0405 at 465.046 kb on + strand at 465.131 kb on + strand at 465.131 kb on + strand at 465.141 kb on - strand at 465.289 kb on - strand, within Shewana3_0406 at 465.344 kb on + strand, within Shewana3_0406 at 465.395 kb on - strand, within Shewana3_0406 at 465.397 kb on + strand, within Shewana3_0406 at 465.573 kb on + strand, within Shewana3_0406 at 465.692 kb on + strand, within Shewana3_0406 at 465.708 kb on + strand, within Shewana3_0406 at 465.853 kb on - strand, within Shewana3_0406 at 465.905 kb on - strand, within Shewana3_0406 at 465.955 kb on - strand, within Shewana3_0406 at 465.974 kb on - strand at 465.974 kb on - strand at 466.251 kb on + strand at 466.374 kb on - strand, within Shewana3_0407 at 466.606 kb on + strand, within Shewana3_0407 at 466.716 kb on + strand, within Shewana3_0407 at 466.716 kb on + strand, within Shewana3_0407 at 466.716 kb on - strand, within Shewana3_0407 at 466.885 kb on - strand, within Shewana3_0407 at 466.936 kb on + strand, within Shewana3_0407 at 466.937 kb on + strand, within Shewana3_0407
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 464,103 - frdB Shewana3_0404 0.19 -1.3 464,375 - frdB Shewana3_0404 0.56 -1.3 464,452 - frdB Shewana3_0404 0.67 -1.1 464,479 - frdB Shewana3_0404 0.70 -0.9 464,539 + frdB Shewana3_0404 0.79 -0.3 464,573 + frdB Shewana3_0404 0.83 +0.3 464,578 + frdB Shewana3_0404 0.84 +2.9 464,773 - +0.1 464,792 + Shewana3_0405 0.12 -0.9 464,800 - Shewana3_0405 0.14 +0.6 464,810 - Shewana3_0405 0.18 -0.4 464,905 + Shewana3_0405 0.51 -3.4 464,913 + Shewana3_0405 0.54 -0.5 464,937 - Shewana3_0405 0.62 -0.3 464,958 + Shewana3_0405 0.70 -1.3 464,966 - Shewana3_0405 0.73 -0.1 464,971 + Shewana3_0405 0.74 -1.0 464,979 - Shewana3_0405 0.77 -0.7 464,979 - Shewana3_0405 0.77 -0.1 464,991 + Shewana3_0405 0.81 -0.1 465,046 + -0.2 465,131 + -0.3 465,131 + -0.3 465,141 - +0.2 465,289 - Shewana3_0406 0.24 +0.3 465,344 + Shewana3_0406 0.30 -2.0 465,395 - Shewana3_0406 0.35 -0.7 465,397 + Shewana3_0406 0.35 +0.7 465,573 + Shewana3_0406 0.52 -0.5 465,692 + Shewana3_0406 0.64 -2.5 465,708 + Shewana3_0406 0.65 -1.4 465,853 - Shewana3_0406 0.79 -1.1 465,905 - Shewana3_0406 0.85 -0.3 465,955 - Shewana3_0406 0.89 -0.0 465,974 - -0.3 465,974 - +0.9 466,251 + -2.5 466,374 - Shewana3_0407 0.21 -1.1 466,606 + Shewana3_0407 0.46 -1.1 466,716 + Shewana3_0407 0.57 -0.4 466,716 + Shewana3_0407 0.57 -0.4 466,716 - Shewana3_0407 0.57 -0.2 466,885 - Shewana3_0407 0.75 -1.0 466,936 + Shewana3_0407 0.81 -1.5 466,937 + Shewana3_0407 0.81 -0.7
Or see this region's nucleotide sequence