Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0401 and Shewana3_0402 are separated by 206 nucleotides Shewana3_0402 and Shewana3_0403 are separated by 61 nucleotides Shewana3_0403 and frdB overlap by 1 nucleotides frdB and Shewana3_0405 are separated by 63 nucleotides
Shewana3_0401: Shewana3_0401 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 460,293 to 461,018
_0401
Shewana3_0402: Shewana3_0402 - fumarate reductase respiratory complex, transmembrane subunit (RefSeq), at 461,225 to 461,896
_0402
Shewana3_0403: Shewana3_0403 - fumarate reductase flavoprotein subunit (RefSeq), at 461,958 to 463,964
_0403
Shewana3_0404: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 463,964 to 464,695
frdB
Shewana3_0405: Shewana3_0405 - antibiotic biosynthesis monooxygenase (RefSeq), at 464,759 to 465,043
_0405
Position (kb) 461
462
463
464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 460.960 kb on + strand at 460.983 kb on - strand at 460.995 kb on + strand at 461.037 kb on + strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.365 kb on - strand, within Shewana3_0402 at 461.370 kb on - strand, within Shewana3_0402 at 461.387 kb on - strand, within Shewana3_0402 at 461.418 kb on + strand, within Shewana3_0402 at 461.470 kb on - strand, within Shewana3_0402 at 461.535 kb on + strand, within Shewana3_0402 at 461.566 kb on + strand, within Shewana3_0402 at 461.571 kb on + strand, within Shewana3_0402 at 461.670 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.942 kb on + strand at 462.015 kb on - strand at 462.025 kb on + strand at 462.103 kb on + strand at 462.237 kb on + strand, within Shewana3_0403 at 462.505 kb on + strand, within Shewana3_0403 at 462.550 kb on + strand, within Shewana3_0403 at 462.558 kb on - strand, within Shewana3_0403 at 462.630 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.663 kb on + strand, within Shewana3_0403 at 462.664 kb on - strand, within Shewana3_0403 at 462.699 kb on - strand, within Shewana3_0403 at 462.707 kb on + strand, within Shewana3_0403 at 462.797 kb on + strand, within Shewana3_0403 at 462.953 kb on + strand, within Shewana3_0403 at 463.026 kb on - strand, within Shewana3_0403 at 463.112 kb on + strand, within Shewana3_0403 at 463.127 kb on - strand, within Shewana3_0403 at 463.147 kb on + strand, within Shewana3_0403 at 463.348 kb on + strand, within Shewana3_0403 at 463.411 kb on + strand, within Shewana3_0403 at 463.435 kb on - strand, within Shewana3_0403 at 463.518 kb on + strand, within Shewana3_0403 at 463.538 kb on + strand, within Shewana3_0403 at 463.596 kb on + strand, within Shewana3_0403 at 463.775 kb on + strand at 464.103 kb on - strand, within frdB at 464.375 kb on - strand, within frdB at 464.452 kb on - strand, within frdB at 464.479 kb on - strand, within frdB at 464.539 kb on + strand, within frdB at 464.573 kb on + strand, within frdB at 464.578 kb on + strand, within frdB at 464.773 kb on - strand at 464.792 kb on + strand, within Shewana3_0405 at 464.800 kb on - strand, within Shewana3_0405 at 464.810 kb on - strand, within Shewana3_0405 at 464.905 kb on + strand, within Shewana3_0405 at 464.913 kb on + strand, within Shewana3_0405 at 464.937 kb on - strand, within Shewana3_0405 at 464.958 kb on + strand, within Shewana3_0405
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 460,960 + +0.1 460,983 - -1.6 460,995 + -0.3 461,037 + -0.3 461,200 - +1.1 461,200 - -1.3 461,200 - +0.2 461,365 - Shewana3_0402 0.21 -1.4 461,370 - Shewana3_0402 0.22 -1.0 461,387 - Shewana3_0402 0.24 -1.0 461,418 + Shewana3_0402 0.29 +0.2 461,470 - Shewana3_0402 0.36 -0.4 461,535 + Shewana3_0402 0.46 -0.6 461,566 + Shewana3_0402 0.51 +0.8 461,571 + Shewana3_0402 0.51 +0.4 461,670 + Shewana3_0402 0.66 +0.0 461,765 + Shewana3_0402 0.80 -0.2 461,765 + Shewana3_0402 0.80 +0.2 461,942 + -0.8 462,015 - -1.1 462,025 + +0.7 462,103 + -1.0 462,237 + Shewana3_0403 0.14 -0.0 462,505 + Shewana3_0403 0.27 +0.7 462,550 + Shewana3_0403 0.29 -0.1 462,558 - Shewana3_0403 0.30 -0.1 462,630 + Shewana3_0403 0.33 -0.5 462,657 + Shewana3_0403 0.35 -0.6 462,657 + Shewana3_0403 0.35 -2.7 462,663 + Shewana3_0403 0.35 -0.2 462,664 - Shewana3_0403 0.35 -0.7 462,699 - Shewana3_0403 0.37 -0.2 462,707 + Shewana3_0403 0.37 -0.2 462,797 + Shewana3_0403 0.42 -0.3 462,953 + Shewana3_0403 0.50 +0.1 463,026 - Shewana3_0403 0.53 +1.4 463,112 + Shewana3_0403 0.57 -1.0 463,127 - Shewana3_0403 0.58 -0.6 463,147 + Shewana3_0403 0.59 -0.3 463,348 + Shewana3_0403 0.69 -0.1 463,411 + Shewana3_0403 0.72 -0.7 463,435 - Shewana3_0403 0.74 +0.3 463,518 + Shewana3_0403 0.78 -0.7 463,538 + Shewana3_0403 0.79 +0.6 463,596 + Shewana3_0403 0.82 -0.3 463,775 + +0.3 464,103 - frdB Shewana3_0404 0.19 -1.3 464,375 - frdB Shewana3_0404 0.56 -1.3 464,452 - frdB Shewana3_0404 0.67 -1.1 464,479 - frdB Shewana3_0404 0.70 -0.9 464,539 + frdB Shewana3_0404 0.79 -0.3 464,573 + frdB Shewana3_0404 0.83 +0.3 464,578 + frdB Shewana3_0404 0.84 +2.9 464,773 - +0.1 464,792 + Shewana3_0405 0.12 -0.9 464,800 - Shewana3_0405 0.14 +0.6 464,810 - Shewana3_0405 0.18 -0.4 464,905 + Shewana3_0405 0.51 -3.4 464,913 + Shewana3_0405 0.54 -0.5 464,937 - Shewana3_0405 0.62 -0.3 464,958 + Shewana3_0405 0.70 -1.3
Or see this region's nucleotide sequence