Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0397 and fis are separated by 254 nucleotides fis and Shewana3_0399 are separated by 16 nucleotides Shewana3_0399 and prmA are separated by 174 nucleotides
Shewana3_0397: Shewana3_0397 - hemerythrin HHE cation binding domain-containing protein (RefSeq), at 456,503 to 457,249
_0397
Shewana3_0398: fis - DNA-binding protein Fis (RefSeq), at 457,504 to 457,809
fis
Shewana3_0399: Shewana3_0399 - nifR3 family TIM-barrel protein (RefSeq), at 457,826 to 458,794
_0399
Shewana3_0400: prmA - ribosomal protein L11 methyltransferase (RefSeq), at 458,969 to 459,850
prmA
Position (kb) 457
458
459 Strain fitness (log2 ratio) -1
0
1 at 457.058 kb on - strand, within Shewana3_0397 at 457.111 kb on - strand, within Shewana3_0397 at 457.177 kb on + strand at 457.193 kb on + strand at 457.235 kb on - strand at 457.395 kb on - strand at 457.547 kb on - strand, within fis at 458.191 kb on - strand, within Shewana3_0399 at 458.368 kb on - strand, within Shewana3_0399 at 458.463 kb on - strand, within Shewana3_0399 at 458.543 kb on - strand, within Shewana3_0399 at 458.692 kb on - strand, within Shewana3_0399 at 458.789 kb on - strand at 458.819 kb on + strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.968 kb on - strand at 459.077 kb on - strand, within prmA at 459.143 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.576 kb on + strand, within prmA at 459.666 kb on + strand, within prmA at 459.751 kb on + strand, within prmA at 459.759 kb on - strand, within prmA at 459.759 kb on - strand, within prmA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 457,058 - Shewana3_0397 0.74 +0.1 457,111 - Shewana3_0397 0.81 -0.3 457,177 + -0.7 457,193 + -0.5 457,235 - +0.0 457,395 - -1.1 457,547 - fis Shewana3_0398 0.14 -0.8 458,191 - Shewana3_0399 0.38 +0.9 458,368 - Shewana3_0399 0.56 +0.9 458,463 - Shewana3_0399 0.66 +0.0 458,543 - Shewana3_0399 0.74 +1.0 458,692 - Shewana3_0399 0.89 +0.6 458,789 - -0.2 458,819 + -0.5 458,822 - +0.4 458,822 - -0.2 458,822 - +0.3 458,968 - +0.4 459,077 - prmA Shewana3_0400 0.12 +0.4 459,143 - prmA Shewana3_0400 0.20 -0.2 459,390 - prmA Shewana3_0400 0.48 -0.2 459,390 - prmA Shewana3_0400 0.48 +0.0 459,390 - prmA Shewana3_0400 0.48 -0.0 459,576 + prmA Shewana3_0400 0.69 -1.3 459,666 + prmA Shewana3_0400 0.79 +0.1 459,751 + prmA Shewana3_0400 0.89 +0.1 459,759 - prmA Shewana3_0400 0.90 -0.2 459,759 - prmA Shewana3_0400 0.90 -0.2
Or see this region's nucleotide sequence