Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0350 and Shewana3_0351 are separated by 151 nucleotides Shewana3_0351 and Shewana3_0352 overlap by 38 nucleotides Shewana3_0352 and Shewana3_0353 are separated by 172 nucleotides
Shewana3_0350: Shewana3_0350 - diguanylate cyclase (RefSeq), at 384,315 to 386,132
_0350
Shewana3_0351: Shewana3_0351 - hypothetical protein (RefSeq), at 386,284 to 386,697
_0351
Shewana3_0352: Shewana3_0352 - amino acid ABC transporter periplasmic protein (RefSeq), at 386,660 to 387,499
_0352
Shewana3_0353: Shewana3_0353 - hypothetical protein (RefSeq), at 387,672 to 388,988
_0353
Position (kb) 386
387 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 385.334 kb on - strand, within Shewana3_0350 at 385.401 kb on + strand, within Shewana3_0350 at 385.546 kb on + strand, within Shewana3_0350 at 385.554 kb on - strand, within Shewana3_0350 at 385.647 kb on - strand, within Shewana3_0350 at 385.775 kb on + strand, within Shewana3_0350 at 385.783 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.841 kb on + strand, within Shewana3_0350 at 385.895 kb on + strand, within Shewana3_0350 at 385.903 kb on - strand, within Shewana3_0350 at 385.924 kb on - strand, within Shewana3_0350 at 386.041 kb on + strand at 386.050 kb on + strand at 386.050 kb on + strand at 386.059 kb on - strand at 386.113 kb on - strand at 386.118 kb on - strand at 386.276 kb on + strand at 386.284 kb on - strand at 386.368 kb on + strand, within Shewana3_0351 at 386.373 kb on + strand, within Shewana3_0351 at 386.436 kb on - strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.759 kb on - strand, within Shewana3_0352 at 386.830 kb on + strand, within Shewana3_0352 at 386.887 kb on + strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 387.045 kb on + strand, within Shewana3_0352 at 387.169 kb on - strand, within Shewana3_0352 at 387.242 kb on + strand, within Shewana3_0352 at 387.260 kb on - strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.408 kb on - strand, within Shewana3_0352 at 387.427 kb on - strand at 387.467 kb on - strand at 387.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 385,334 - Shewana3_0350 0.56 +0.1 385,401 + Shewana3_0350 0.60 -0.7 385,546 + Shewana3_0350 0.68 -0.6 385,554 - Shewana3_0350 0.68 -0.5 385,647 - Shewana3_0350 0.73 -0.5 385,775 + Shewana3_0350 0.80 -0.8 385,783 - Shewana3_0350 0.81 +0.2 385,831 - Shewana3_0350 0.83 -0.0 385,831 - Shewana3_0350 0.83 -0.3 385,841 + Shewana3_0350 0.84 -0.3 385,895 + Shewana3_0350 0.87 -1.0 385,903 - Shewana3_0350 0.87 -0.2 385,924 - Shewana3_0350 0.89 +0.3 386,041 + +0.9 386,050 + +0.7 386,050 + -0.4 386,059 - -0.6 386,113 - +0.8 386,118 - -0.3 386,276 + -0.1 386,284 - -0.0 386,368 + Shewana3_0351 0.20 +0.3 386,373 + Shewana3_0351 0.21 -0.8 386,436 - Shewana3_0351 0.37 -0.3 386,624 + Shewana3_0351 0.82 -1.0 386,624 + Shewana3_0351 0.82 +4.5 386,759 - Shewana3_0352 0.12 -0.4 386,830 + Shewana3_0352 0.20 -0.7 386,887 + Shewana3_0352 0.27 +0.0 386,895 - Shewana3_0352 0.28 -1.1 386,895 - Shewana3_0352 0.28 -0.3 386,895 - Shewana3_0352 0.28 -1.4 387,045 + Shewana3_0352 0.46 -1.5 387,169 - Shewana3_0352 0.61 -1.1 387,242 + Shewana3_0352 0.69 -1.3 387,260 - Shewana3_0352 0.71 +4.5 387,286 + Shewana3_0352 0.75 -0.3 387,286 + Shewana3_0352 0.75 -0.4 387,286 + Shewana3_0352 0.75 -0.7 387,286 + Shewana3_0352 0.75 -1.2 387,294 - Shewana3_0352 0.75 -0.6 387,294 - Shewana3_0352 0.75 -0.2 387,408 - Shewana3_0352 0.89 -1.5 387,427 - -2.4 387,467 - -1.2 387,472 - -1.4
Or see this region's nucleotide sequence