Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0017 and Shewana3_R0018 are separated by 174 nucleotides Shewana3_R0018 and Shewana3_R0019 are separated by 42 nucleotides Shewana3_R0019 and Shewana3_0239 are separated by 205 nucleotides Shewana3_0239 and Shewana3_0240 are separated by 38 nucleotides
Shewana3_R0017: Shewana3_R0017 - 23S ribosomal RNA (RefSeq), at 269,160 to 272,054
_R0017
Shewana3_R0018: Shewana3_R0018 - 5S ribosomal RNA (RefSeq), at 272,229 to 272,348
_R0018
Shewana3_R0019: Shewana3_R0019 - tRNA-Asp (RefSeq), at 272,391 to 272,467
_R0019
Shewana3_0239: Shewana3_0239 - hypothetical protein (RefSeq), at 272,673 to 273,185
_0239
Shewana3_0240: Shewana3_0240 - ATPase domain-containing protein (RefSeq), at 273,224 to 274,540
_0240
Position (kb)
272
273
274 Strain fitness (log2 ratio)
-2
-1
0
1 at 273.051 kb on + strand, within Shewana3_0239 at 273.215 kb on + strand at 273.262 kb on - strand at 273.331 kb on + strand at 273.423 kb on + strand, within Shewana3_0240 at 273.431 kb on - strand, within Shewana3_0240 at 273.479 kb on - strand, within Shewana3_0240 at 273.611 kb on - strand, within Shewana3_0240 at 273.720 kb on - strand, within Shewana3_0240 at 273.825 kb on + strand, within Shewana3_0240 at 273.838 kb on - strand, within Shewana3_0240 at 273.991 kb on + strand, within Shewana3_0240 at 274.079 kb on - strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 273,051 + Shewana3_0239 0.74 -0.4 273,215 + +1.7 273,262 - +0.9 273,331 + -1.1 273,423 + Shewana3_0240 0.15 -0.6 273,431 - Shewana3_0240 0.16 -1.4 273,479 - Shewana3_0240 0.19 -1.8 273,611 - Shewana3_0240 0.29 -0.3 273,720 - Shewana3_0240 0.38 -0.5 273,825 + Shewana3_0240 0.46 -1.5 273,838 - Shewana3_0240 0.47 -0.7 273,991 + Shewana3_0240 0.58 -0.2 274,079 - Shewana3_0240 0.65 -1.1 274,126 + Shewana3_0240 0.68 -0.6 274,126 + Shewana3_0240 0.68 +0.5
Or see this region's nucleotide sequence