Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0233 and Shewana3_0234 are separated by 399 nucleotides Shewana3_0234 and Shewana3_0235 are separated by 11 nucleotides Shewana3_0235 and Shewana3_0236 overlap by 4 nucleotides
Shewana3_0233: Shewana3_0233 - TPR repeat-containing protein (RefSeq), at 261,588 to 262,835
_0233
Shewana3_0234: Shewana3_0234 - cytochrome c-type biogenesis protein CcmF (RefSeq), at 263,235 to 265,214
_0234
Shewana3_0235: Shewana3_0235 - periplasmic protein thiol--disulphide oxidoreductase DsbE (RefSeq), at 265,226 to 265,780
_0235
Shewana3_0236: Shewana3_0236 - cytochrome C biogenesis protein (RefSeq), at 265,777 to 266,256
_0236
Position (kb) 263
264
265
266 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 262.247 kb on - strand, within Shewana3_0233 at 262.317 kb on + strand, within Shewana3_0233 at 262.402 kb on + strand, within Shewana3_0233 at 262.591 kb on - strand, within Shewana3_0233 at 262.655 kb on + strand, within Shewana3_0233 at 262.702 kb on + strand, within Shewana3_0233 at 262.741 kb on - strand at 262.743 kb on - strand at 262.769 kb on + strand at 262.792 kb on - strand at 262.792 kb on - strand at 262.797 kb on - strand at 262.936 kb on + strand at 262.944 kb on - strand at 262.944 kb on - strand at 263.264 kb on + strand at 263.264 kb on + strand at 263.272 kb on - strand at 263.414 kb on - strand at 263.421 kb on + strand at 263.443 kb on + strand, within Shewana3_0234 at 263.495 kb on + strand, within Shewana3_0234 at 263.514 kb on - strand, within Shewana3_0234 at 263.528 kb on - strand, within Shewana3_0234 at 263.533 kb on - strand, within Shewana3_0234 at 263.724 kb on - strand, within Shewana3_0234 at 263.757 kb on + strand, within Shewana3_0234 at 263.816 kb on + strand, within Shewana3_0234 at 263.828 kb on + strand, within Shewana3_0234 at 263.851 kb on + strand, within Shewana3_0234 at 264.003 kb on + strand, within Shewana3_0234 at 264.130 kb on + strand, within Shewana3_0234 at 264.247 kb on + strand, within Shewana3_0234 at 264.774 kb on + strand, within Shewana3_0234 at 264.829 kb on + strand, within Shewana3_0234 at 264.829 kb on + strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 265.112 kb on + strand at 265.395 kb on - strand, within Shewana3_0235 at 265.532 kb on - strand, within Shewana3_0235 at 265.562 kb on + strand, within Shewana3_0235 at 265.613 kb on + strand, within Shewana3_0235 at 265.765 kb on + strand at 265.842 kb on + strand, within Shewana3_0236 at 265.851 kb on + strand, within Shewana3_0236 at 265.915 kb on - strand, within Shewana3_0236 at 265.952 kb on + strand, within Shewana3_0236 at 266.018 kb on - strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 262,247 - Shewana3_0233 0.53 +0.1 262,317 + Shewana3_0233 0.58 +0.2 262,402 + Shewana3_0233 0.65 -1.9 262,591 - Shewana3_0233 0.80 -1.0 262,655 + Shewana3_0233 0.85 +0.8 262,702 + Shewana3_0233 0.89 +1.2 262,741 - +0.9 262,743 - +1.1 262,769 + +0.2 262,792 - +0.7 262,792 - +1.2 262,797 - +2.2 262,936 + -0.5 262,944 - -1.8 262,944 - -0.5 263,264 + +1.3 263,264 + +1.0 263,272 - +1.9 263,414 - +0.9 263,421 + +1.0 263,443 + Shewana3_0234 0.11 +0.9 263,495 + Shewana3_0234 0.13 +0.9 263,514 - Shewana3_0234 0.14 -0.3 263,528 - Shewana3_0234 0.15 +1.2 263,533 - Shewana3_0234 0.15 -0.3 263,724 - Shewana3_0234 0.25 -1.8 263,757 + Shewana3_0234 0.26 -0.3 263,816 + Shewana3_0234 0.29 +0.5 263,828 + Shewana3_0234 0.30 +1.5 263,851 + Shewana3_0234 0.31 +0.6 264,003 + Shewana3_0234 0.39 +0.9 264,130 + Shewana3_0234 0.45 +0.4 264,247 + Shewana3_0234 0.51 +1.6 264,774 + Shewana3_0234 0.78 +2.6 264,829 + Shewana3_0234 0.81 +0.2 264,829 + Shewana3_0234 0.81 +0.7 264,874 - Shewana3_0234 0.83 +0.9 264,874 - Shewana3_0234 0.83 +1.2 265,112 + +0.5 265,395 - Shewana3_0235 0.30 +1.5 265,532 - Shewana3_0235 0.55 +0.2 265,562 + Shewana3_0235 0.61 +0.9 265,613 + Shewana3_0235 0.70 +1.4 265,765 + +0.2 265,842 + Shewana3_0236 0.14 +1.5 265,851 + Shewana3_0236 0.15 +0.2 265,915 - Shewana3_0236 0.29 -0.1 265,952 + Shewana3_0236 0.36 +0.5 266,018 - Shewana3_0236 0.50 +3.0 266,113 + Shewana3_0236 0.70 -0.3 266,113 + Shewana3_0236 0.70 +1.2
Or see this region's nucleotide sequence