Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0231 and Shewana3_0232 are separated by 325 nucleotides Shewana3_0232 and Shewana3_0233 are separated by 83 nucleotides Shewana3_0233 and Shewana3_0234 are separated by 399 nucleotides
Shewana3_0231: Shewana3_0231 - cytochrome c biogenesis protein CcmA (RefSeq), at 260,229 to 260,879
_0231
Shewana3_0232: Shewana3_0232 - cytochrome c, class I (RefSeq), at 261,205 to 261,504
_0232
Shewana3_0233: Shewana3_0233 - TPR repeat-containing protein (RefSeq), at 261,588 to 262,835
_0233
Shewana3_0234: Shewana3_0234 - cytochrome c-type biogenesis protein CcmF (RefSeq), at 263,235 to 265,214
_0234
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 260.593 kb on + strand, within Shewana3_0231 at 260.696 kb on + strand, within Shewana3_0231 at 260.697 kb on - strand, within Shewana3_0231 at 260.697 kb on - strand, within Shewana3_0231 at 260.700 kb on + strand, within Shewana3_0231 at 260.704 kb on - strand, within Shewana3_0231 at 260.708 kb on - strand, within Shewana3_0231 at 260.708 kb on - strand, within Shewana3_0231 at 260.835 kb on - strand at 260.846 kb on + strand at 260.955 kb on - strand at 260.955 kb on - strand at 260.993 kb on + strand at 261.129 kb on + strand at 261.130 kb on + strand at 261.147 kb on - strand at 261.246 kb on - strand, within Shewana3_0232 at 261.302 kb on + strand, within Shewana3_0232 at 261.302 kb on - strand, within Shewana3_0232 at 261.302 kb on - strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.391 kb on + strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.399 kb on - strand, within Shewana3_0232 at 261.401 kb on + strand, within Shewana3_0232 at 261.401 kb on + strand, within Shewana3_0232 at 261.548 kb on - strand at 261.565 kb on + strand at 261.573 kb on - strand at 261.596 kb on - strand at 261.624 kb on - strand at 261.752 kb on + strand, within Shewana3_0233 at 261.755 kb on - strand, within Shewana3_0233 at 261.824 kb on + strand, within Shewana3_0233 at 261.854 kb on - strand, within Shewana3_0233 at 261.916 kb on - strand, within Shewana3_0233 at 262.012 kb on + strand, within Shewana3_0233 at 262.192 kb on + strand, within Shewana3_0233 at 262.223 kb on - strand, within Shewana3_0233 at 262.223 kb on - strand, within Shewana3_0233 at 262.223 kb on - strand, within Shewana3_0233 at 262.223 kb on - strand, within Shewana3_0233 at 262.223 kb on - strand, within Shewana3_0233 at 262.247 kb on - strand, within Shewana3_0233 at 262.317 kb on + strand, within Shewana3_0233 at 262.402 kb on + strand, within Shewana3_0233 at 262.591 kb on - strand, within Shewana3_0233 at 262.655 kb on + strand, within Shewana3_0233 at 262.702 kb on + strand, within Shewana3_0233 at 262.741 kb on - strand at 262.743 kb on - strand at 262.769 kb on + strand at 262.792 kb on - strand at 262.792 kb on - strand at 262.797 kb on - strand at 262.936 kb on + strand at 262.944 kb on - strand at 262.944 kb on - strand at 263.264 kb on + strand at 263.264 kb on + strand at 263.272 kb on - strand at 263.414 kb on - strand at 263.421 kb on + strand at 263.443 kb on + strand, within Shewana3_0234 at 263.495 kb on + strand, within Shewana3_0234 at 263.514 kb on - strand, within Shewana3_0234 at 263.528 kb on - strand, within Shewana3_0234 at 263.533 kb on - strand, within Shewana3_0234 at 263.724 kb on - strand, within Shewana3_0234 at 263.757 kb on + strand, within Shewana3_0234 at 263.816 kb on + strand, within Shewana3_0234 at 263.828 kb on + strand, within Shewana3_0234
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 260,593 + Shewana3_0231 0.56 +1.2 260,696 + Shewana3_0231 0.72 +0.6 260,697 - Shewana3_0231 0.72 +0.9 260,697 - Shewana3_0231 0.72 +0.8 260,700 + Shewana3_0231 0.72 +0.7 260,704 - Shewana3_0231 0.73 -0.1 260,708 - Shewana3_0231 0.74 -0.2 260,708 - Shewana3_0231 0.74 +0.6 260,835 - +1.5 260,846 + +0.3 260,955 - -1.7 260,955 - -0.4 260,993 + +0.5 261,129 + +3.9 261,130 + +0.3 261,147 - -1.2 261,246 - Shewana3_0232 0.14 +0.0 261,302 + Shewana3_0232 0.32 +0.8 261,302 - Shewana3_0232 0.32 -0.5 261,302 - Shewana3_0232 0.32 +0.1 261,391 + Shewana3_0232 0.62 -0.0 261,391 + Shewana3_0232 0.62 +1.2 261,391 + Shewana3_0232 0.62 +0.7 261,391 + Shewana3_0232 0.62 +1.0 261,391 + Shewana3_0232 0.62 +2.1 261,391 + Shewana3_0232 0.62 +0.9 261,391 + Shewana3_0232 0.62 +0.9 261,391 + Shewana3_0232 0.62 +0.7 261,391 + Shewana3_0232 0.62 +3.4 261,391 + Shewana3_0232 0.62 +0.6 261,399 - Shewana3_0232 0.65 +1.5 261,399 - Shewana3_0232 0.65 -1.1 261,399 - Shewana3_0232 0.65 +0.4 261,399 - Shewana3_0232 0.65 +0.5 261,399 - Shewana3_0232 0.65 -0.7 261,399 - Shewana3_0232 0.65 +0.9 261,399 - Shewana3_0232 0.65 -0.1 261,401 + Shewana3_0232 0.65 +0.4 261,401 + Shewana3_0232 0.65 +0.8 261,548 - -0.6 261,565 + -1.5 261,573 - +0.6 261,596 - -1.0 261,624 - +0.5 261,752 + Shewana3_0233 0.13 +0.5 261,755 - Shewana3_0233 0.13 +0.1 261,824 + Shewana3_0233 0.19 -2.9 261,854 - Shewana3_0233 0.21 +0.2 261,916 - Shewana3_0233 0.26 +0.1 262,012 + Shewana3_0233 0.34 -1.1 262,192 + Shewana3_0233 0.48 -1.6 262,223 - Shewana3_0233 0.51 +0.1 262,223 - Shewana3_0233 0.51 +0.4 262,223 - Shewana3_0233 0.51 +0.5 262,223 - Shewana3_0233 0.51 +4.1 262,223 - Shewana3_0233 0.51 -0.3 262,247 - Shewana3_0233 0.53 +0.1 262,317 + Shewana3_0233 0.58 +0.2 262,402 + Shewana3_0233 0.65 -1.9 262,591 - Shewana3_0233 0.80 -1.0 262,655 + Shewana3_0233 0.85 +0.8 262,702 + Shewana3_0233 0.89 +1.2 262,741 - +0.9 262,743 - +1.1 262,769 + +0.2 262,792 - +0.7 262,792 - +1.2 262,797 - +2.2 262,936 + -0.5 262,944 - -1.8 262,944 - -0.5 263,264 + +1.3 263,264 + +1.0 263,272 - +1.9 263,414 - +0.9 263,421 + +1.0 263,443 + Shewana3_0234 0.11 +0.9 263,495 + Shewana3_0234 0.13 +0.9 263,514 - Shewana3_0234 0.14 -0.3 263,528 - Shewana3_0234 0.15 +1.2 263,533 - Shewana3_0234 0.15 -0.3 263,724 - Shewana3_0234 0.25 -1.8 263,757 + Shewana3_0234 0.26 -0.3 263,816 + Shewana3_0234 0.29 +0.5 263,828 + Shewana3_0234 0.30 +1.5
Or see this region's nucleotide sequence