Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0047 and Shewana3_0048 overlap by 26 nucleotides Shewana3_0048 and Shewana3_0049 are separated by 12 nucleotides Shewana3_0049 and Shewana3_0050 are separated by 48 nucleotides
Shewana3_0047: Shewana3_0047 - BadM/Rrf2 family transcriptional regulator (RefSeq), at 56,630 to 57,085
_0047
Shewana3_0048: Shewana3_0048 - protein of unknown function DUF610, YibQ (RefSeq), at 57,060 to 57,815
_0048
Shewana3_0049: Shewana3_0049 - C-terminal processing peptidase-3 (RefSeq), at 57,828 to 59,033
_0049
Shewana3_0050: Shewana3_0050 - peptidase M23B (RefSeq), at 59,082 to 60,215
_0050
Position (kb) 57
58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.215 kb on + strand, within Shewana3_0048 at 57.223 kb on - strand, within Shewana3_0048 at 57.283 kb on - strand, within Shewana3_0048 at 57.401 kb on + strand, within Shewana3_0048 at 57.403 kb on - strand, within Shewana3_0048 at 57.431 kb on - strand, within Shewana3_0048 at 57.569 kb on - strand, within Shewana3_0048 at 58.016 kb on - strand, within Shewana3_0049 at 58.089 kb on + strand, within Shewana3_0049 at 58.255 kb on + strand, within Shewana3_0049 at 58.376 kb on + strand, within Shewana3_0049 at 58.376 kb on + strand, within Shewana3_0049 at 58.384 kb on - strand, within Shewana3_0049 at 58.384 kb on - strand, within Shewana3_0049 at 58.384 kb on - strand, within Shewana3_0049 at 58.384 kb on - strand, within Shewana3_0049 at 58.540 kb on - strand, within Shewana3_0049 at 58.544 kb on + strand, within Shewana3_0049 at 58.704 kb on + strand, within Shewana3_0049 at 58.979 kb on + strand at 58.979 kb on + strand at 59.136 kb on + strand at 59.136 kb on + strand at 59.301 kb on - strand, within Shewana3_0050 at 59.302 kb on - strand, within Shewana3_0050
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 57,215 + Shewana3_0048 0.21 -2.1 57,223 - Shewana3_0048 0.22 -0.6 57,283 - Shewana3_0048 0.29 +2.4 57,401 + Shewana3_0048 0.45 -0.1 57,403 - Shewana3_0048 0.45 -1.0 57,431 - Shewana3_0048 0.49 -1.9 57,569 - Shewana3_0048 0.67 -0.5 58,016 - Shewana3_0049 0.16 -0.7 58,089 + Shewana3_0049 0.22 -0.9 58,255 + Shewana3_0049 0.35 -1.5 58,376 + Shewana3_0049 0.45 -0.8 58,376 + Shewana3_0049 0.45 -1.0 58,384 - Shewana3_0049 0.46 -0.5 58,384 - Shewana3_0049 0.46 -1.6 58,384 - Shewana3_0049 0.46 -0.2 58,384 - Shewana3_0049 0.46 -2.2 58,540 - Shewana3_0049 0.59 -0.4 58,544 + Shewana3_0049 0.59 -0.8 58,704 + Shewana3_0049 0.73 -0.0 58,979 + -0.8 58,979 + -1.7 59,136 + -1.6 59,136 + +0.4 59,301 - Shewana3_0050 0.19 -1.0 59,302 - Shewana3_0050 0.19 +0.7
Or see this region's nucleotide sequence