Experiment: nitrate 2000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0645 and Shew_0646 overlap by 4 nucleotides Shew_0646 and Shew_0647 are separated by 14 nucleotides Shew_0647 and Shew_0648 are separated by 7 nucleotides Shew_0648 and Shew_0649 are separated by 4 nucleotides
Shew_0645: Shew_0645 - type II and III secretion system protein (RefSeq), at 733,888 to 735,198
_0645
Shew_0646: Shew_0646 - SAF domain-containing protein (RefSeq), at 735,195 to 736,091
_0646
Shew_0647: Shew_0647 - hypothetical protein (RefSeq), at 736,106 to 736,297
_0647
Shew_0648: Shew_0648 - hypothetical protein (RefSeq), at 736,305 to 736,976
_0648
Shew_0649: Shew_0649 - hypothetical protein (RefSeq), at 736,981 to 737,841
_0649
Position (kb) 735
736
737 Strain fitness (log2 ratio) -1
0
1
2 at 734.690 kb on + strand, within Shew_0645 at 734.931 kb on + strand, within Shew_0645 at 735.233 kb on - strand at 735.270 kb on + strand at 735.539 kb on + strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.952 kb on + strand, within Shew_0646 at 736.189 kb on - strand, within Shew_0647 at 736.217 kb on - strand, within Shew_0647 at 736.292 kb on + strand at 736.439 kb on + strand, within Shew_0648 at 736.586 kb on + strand, within Shew_0648 at 736.836 kb on - strand, within Shew_0648 at 736.936 kb on - strand at 736.991 kb on - strand at 737.069 kb on + strand, within Shew_0649
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 2000 mM remove 734,690 + Shew_0645 0.61 -0.2 734,931 + Shew_0645 0.80 -0.1 735,233 - +0.7 735,270 + +0.1 735,539 + Shew_0646 0.38 +0.4 735,875 - Shew_0646 0.76 +0.2 735,875 - Shew_0646 0.76 +1.2 735,952 + Shew_0646 0.84 +0.9 736,189 - Shew_0647 0.43 +0.7 736,217 - Shew_0647 0.58 +0.4 736,292 + +0.2 736,439 + Shew_0648 0.20 +0.7 736,586 + Shew_0648 0.42 +0.3 736,836 - Shew_0648 0.79 +0.3 736,936 - +0.0 736,991 - +2.1 737,069 + Shew_0649 0.10 +0.1
Or see this region's nucleotide sequence