Experiment: Aluminum chloride 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5742 and Pf6N2E2_5743 are separated by 35 nucleotides Pf6N2E2_5743 and Pf6N2E2_5744 overlap by 4 nucleotides
Pf6N2E2_5742 - Cobyric acid synthase (EC 6.3.5.10), at 6,558,721 to 6,560,172
_5742
Pf6N2E2_5743 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 6,560,208 to 6,560,729
_5743
Pf6N2E2_5744 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 6,560,726 to 6,561,781
_5744
Position (kb)
6560
6561 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6559.230 kb on - strand, within Pf6N2E2_5742 at 6559.393 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742 at 6559.633 kb on + strand, within Pf6N2E2_5742 at 6559.933 kb on + strand, within Pf6N2E2_5742 at 6559.934 kb on - strand, within Pf6N2E2_5742 at 6559.993 kb on + strand, within Pf6N2E2_5742 at 6559.993 kb on + strand, within Pf6N2E2_5742 at 6559.993 kb on + strand, within Pf6N2E2_5742 at 6559.993 kb on + strand, within Pf6N2E2_5742 at 6559.993 kb on + strand, within Pf6N2E2_5742 at 6559.994 kb on - strand, within Pf6N2E2_5742 at 6559.994 kb on - strand, within Pf6N2E2_5742 at 6559.994 kb on - strand, within Pf6N2E2_5742 at 6559.994 kb on - strand, within Pf6N2E2_5742 at 6559.994 kb on - strand, within Pf6N2E2_5742 at 6560.077 kb on + strand at 6560.078 kb on - strand at 6560.295 kb on + strand, within Pf6N2E2_5743 at 6560.295 kb on + strand, within Pf6N2E2_5743 at 6560.295 kb on + strand, within Pf6N2E2_5743 at 6560.295 kb on + strand, within Pf6N2E2_5743 at 6560.295 kb on + strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.296 kb on - strand, within Pf6N2E2_5743 at 6560.622 kb on + strand, within Pf6N2E2_5743 at 6560.717 kb on + strand at 6560.725 kb on + strand at 6561.474 kb on - strand, within Pf6N2E2_5744
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 4 mM remove 6,559,230 - Pf6N2E2_5742 0.35 +2.0 6,559,393 + Pf6N2E2_5742 0.46 +1.5 6,559,633 + Pf6N2E2_5742 0.63 -0.9 6,559,633 + Pf6N2E2_5742 0.63 -0.1 6,559,633 + Pf6N2E2_5742 0.63 +1.6 6,559,933 + Pf6N2E2_5742 0.83 -0.3 6,559,934 - Pf6N2E2_5742 0.84 +1.2 6,559,993 + Pf6N2E2_5742 0.88 +0.6 6,559,993 + Pf6N2E2_5742 0.88 +2.4 6,559,993 + Pf6N2E2_5742 0.88 +2.0 6,559,993 + Pf6N2E2_5742 0.88 +0.5 6,559,993 + Pf6N2E2_5742 0.88 +1.1 6,559,994 - Pf6N2E2_5742 0.88 +2.0 6,559,994 - Pf6N2E2_5742 0.88 -0.2 6,559,994 - Pf6N2E2_5742 0.88 +1.1 6,559,994 - Pf6N2E2_5742 0.88 +2.3 6,559,994 - Pf6N2E2_5742 0.88 +2.2 6,560,077 + +2.0 6,560,078 - +1.0 6,560,295 + Pf6N2E2_5743 0.17 +1.2 6,560,295 + Pf6N2E2_5743 0.17 +0.2 6,560,295 + Pf6N2E2_5743 0.17 +0.5 6,560,295 + Pf6N2E2_5743 0.17 +1.7 6,560,295 + Pf6N2E2_5743 0.17 +1.9 6,560,296 - Pf6N2E2_5743 0.17 +1.4 6,560,296 - Pf6N2E2_5743 0.17 +0.9 6,560,296 - Pf6N2E2_5743 0.17 +2.0 6,560,296 - Pf6N2E2_5743 0.17 +1.1 6,560,296 - Pf6N2E2_5743 0.17 +1.4 6,560,296 - Pf6N2E2_5743 0.17 +1.6 6,560,296 - Pf6N2E2_5743 0.17 +1.3 6,560,296 - Pf6N2E2_5743 0.17 -0.1 6,560,296 - Pf6N2E2_5743 0.17 -1.4 6,560,622 + Pf6N2E2_5743 0.79 -0.1 6,560,717 + +1.3 6,560,725 + +0.6 6,561,474 - Pf6N2E2_5744 0.71 +2.9
Or see this region's nucleotide sequence