Experiment: Aluminum chloride 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_422 and Pf6N2E2_423 are separated by 20 nucleotides Pf6N2E2_423 and Pf6N2E2_424 are separated by 129 nucleotides
Pf6N2E2_422 - Na+ driven multidrug efflux pump, at 490,565 to 491,863
_422
Pf6N2E2_423 - Putative phosphatase YfbT, at 491,884 to 492,588
_423
Pf6N2E2_424 - Decarboxylase family protein, at 492,718 to 494,091
_424
Position (kb) 491
492
493 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 490.904 kb on + strand, within Pf6N2E2_422 at 490.904 kb on + strand, within Pf6N2E2_422 at 490.905 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 491.241 kb on + strand, within Pf6N2E2_422 at 491.241 kb on + strand, within Pf6N2E2_422 at 491.242 kb on - strand, within Pf6N2E2_422 at 491.312 kb on - strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand at 491.495 kb on - strand, within Pf6N2E2_422 at 491.519 kb on + strand, within Pf6N2E2_422 at 491.519 kb on + strand, within Pf6N2E2_422 at 491.686 kb on + strand, within Pf6N2E2_422 at 491.687 kb on - strand, within Pf6N2E2_422 at 491.729 kb on + strand, within Pf6N2E2_422 at 491.730 kb on - strand, within Pf6N2E2_422 at 491.786 kb on + strand at 491.787 kb on - strand at 491.817 kb on - strand at 491.819 kb on + strand at 491.820 kb on - strand at 491.861 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 492.279 kb on + strand, within Pf6N2E2_423 at 492.486 kb on + strand, within Pf6N2E2_423 at 492.526 kb on + strand at 492.526 kb on + strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.584 kb on + strand at 492.584 kb on + strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.660 kb on + strand at 492.714 kb on + strand at 492.715 kb on - strand at 492.746 kb on + strand at 492.746 kb on + strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand at 493.143 kb on - strand, within Pf6N2E2_424 at 493.143 kb on - strand, within Pf6N2E2_424 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.349 kb on - strand, within Pf6N2E2_424 at 493.470 kb on + strand, within Pf6N2E2_424 at 493.471 kb on - strand, within Pf6N2E2_424 at 493.545 kb on + strand, within Pf6N2E2_424 at 493.546 kb on - strand, within Pf6N2E2_424
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 4 mM remove 490,904 + Pf6N2E2_422 0.26 -1.5 490,904 + Pf6N2E2_422 0.26 +2.1 490,905 - Pf6N2E2_422 0.26 +1.2 490,945 - Pf6N2E2_422 0.29 +0.4 490,945 - Pf6N2E2_422 0.29 +0.5 490,945 - Pf6N2E2_422 0.29 +1.7 491,241 + Pf6N2E2_422 0.52 -0.2 491,241 + Pf6N2E2_422 0.52 -3.3 491,242 - Pf6N2E2_422 0.52 -0.1 491,312 - Pf6N2E2_422 0.58 -1.7 491,366 + Pf6N2E2_422 0.62 +0.9 491,366 + Pf6N2E2_422 0.62 -0.5 491,366 + Pf6N2E2_422 0.62 +0.1 491,366 + Pf6N2E2_422 0.62 -0.0 491,366 + Pf6N2E2_422 0.62 +0.5 491,366 + Pf6N2E2_422 0.62 -0.2 491,366 + Pf6N2E2_422 0.62 -0.5 491,366 + Pf6N2E2_422 0.62 -0.4 491,366 + Pf6N2E2_422 0.62 +1.0 491,366 + Pf6N2E2_422 0.62 -0.8 491,367 - Pf6N2E2_422 0.62 +1.6 491,367 - Pf6N2E2_422 0.62 -0.1 491,367 - Pf6N2E2_422 0.62 +1.4 491,367 - +0.7 491,495 - Pf6N2E2_422 0.72 +1.4 491,519 + Pf6N2E2_422 0.73 +0.6 491,519 + Pf6N2E2_422 0.73 -0.1 491,686 + Pf6N2E2_422 0.86 -2.8 491,687 - Pf6N2E2_422 0.86 -0.1 491,729 + Pf6N2E2_422 0.90 -0.8 491,730 - Pf6N2E2_422 0.90 -1.0 491,786 + +0.2 491,787 - +1.7 491,817 - +2.0 491,819 + +0.2 491,820 - +1.4 491,861 + +0.8 491,877 + +0.8 491,877 + +1.0 491,877 + +0.0 491,877 + +0.5 491,878 - -2.3 491,878 - -0.9 491,878 - -1.3 491,878 - +1.7 492,279 + Pf6N2E2_423 0.56 -1.3 492,486 + Pf6N2E2_423 0.85 -0.3 492,526 + +0.1 492,526 + +0.9 492,527 - +0.2 492,527 - -3.4 492,527 - +1.4 492,584 + -3.1 492,584 + +1.2 492,585 - +0.7 492,585 - +1.0 492,585 - +1.6 492,585 - -1.1 492,660 + +0.0 492,714 + +2.1 492,715 - +1.1 492,746 + +1.0 492,746 + -0.8 492,747 - -0.5 492,747 - +0.5 492,747 - +0.7 492,747 - -0.6 493,143 - Pf6N2E2_424 0.31 +1.2 493,143 - Pf6N2E2_424 0.31 -1.3 493,348 + Pf6N2E2_424 0.46 -4.1 493,348 + Pf6N2E2_424 0.46 +0.3 493,349 - Pf6N2E2_424 0.46 -0.3 493,470 + Pf6N2E2_424 0.55 -0.6 493,471 - Pf6N2E2_424 0.55 +0.4 493,545 + Pf6N2E2_424 0.60 -2.1 493,546 - Pf6N2E2_424 0.60 +0.3
Or see this region's nucleotide sequence