Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1235

Experiment: Aluminum chloride 4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1233 and Pf6N2E2_1234 are separated by 139 nucleotidesPf6N2E2_1234 and Pf6N2E2_1235 are separated by 140 nucleotidesPf6N2E2_1235 and Pf6N2E2_1236 are separated by 341 nucleotides Pf6N2E2_1233 - ABC-type protease exporter, ATP-binding component PrtD/AprD, at 1,444,773 to 1,446,572 _1233 Pf6N2E2_1234 - hypothetical protein, at 1,446,712 to 1,447,065 _1234 Pf6N2E2_1235 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 1,447,206 to 1,448,663 _1235 Pf6N2E2_1236 - Transcriptional regulator, LysR family, at 1,449,005 to 1,449,901 _1236 Position (kb) 1447 1448 1449Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1446.445 kb on + strandat 1446.703 kb on - strandat 1446.703 kb on - strandat 1446.703 kb on - strandat 1446.779 kb on + strand, within Pf6N2E2_1234at 1446.782 kb on + strand, within Pf6N2E2_1234at 1446.782 kb on + strand, within Pf6N2E2_1234at 1447.383 kb on - strand, within Pf6N2E2_1235at 1447.825 kb on + strand, within Pf6N2E2_1235at 1447.825 kb on + strand, within Pf6N2E2_1235at 1447.826 kb on - strand, within Pf6N2E2_1235at 1447.865 kb on - strand, within Pf6N2E2_1235at 1447.865 kb on - strand, within Pf6N2E2_1235at 1447.912 kb on + strand, within Pf6N2E2_1235at 1447.950 kb on - strand, within Pf6N2E2_1235at 1447.966 kb on + strand, within Pf6N2E2_1235at 1447.966 kb on + strand, within Pf6N2E2_1235at 1447.966 kb on + strand, within Pf6N2E2_1235at 1447.967 kb on - strand, within Pf6N2E2_1235at 1447.981 kb on + strand, within Pf6N2E2_1235at 1448.002 kb on + strand, within Pf6N2E2_1235at 1448.002 kb on + strand, within Pf6N2E2_1235at 1448.003 kb on - strand, within Pf6N2E2_1235at 1448.003 kb on - strand, within Pf6N2E2_1235at 1448.017 kb on + strand, within Pf6N2E2_1235at 1448.017 kb on + strand, within Pf6N2E2_1235at 1448.017 kb on + strand, within Pf6N2E2_1235at 1448.017 kb on + strand, within Pf6N2E2_1235at 1448.044 kb on + strand, within Pf6N2E2_1235at 1448.107 kb on + strand, within Pf6N2E2_1235at 1448.107 kb on + strand, within Pf6N2E2_1235at 1448.107 kb on + strand, within Pf6N2E2_1235at 1448.108 kb on - strand, within Pf6N2E2_1235at 1448.140 kb on + strand, within Pf6N2E2_1235at 1448.140 kb on + strand, within Pf6N2E2_1235at 1448.140 kb on + strand, within Pf6N2E2_1235at 1448.140 kb on + strand, within Pf6N2E2_1235at 1448.158 kb on + strandat 1448.200 kb on + strand, within Pf6N2E2_1235at 1448.236 kb on + strand, within Pf6N2E2_1235at 1448.236 kb on + strand, within Pf6N2E2_1235at 1448.236 kb on + strand, within Pf6N2E2_1235at 1448.236 kb on + strand, within Pf6N2E2_1235at 1448.237 kb on - strand, within Pf6N2E2_1235at 1448.237 kb on - strand, within Pf6N2E2_1235at 1448.237 kb on - strand, within Pf6N2E2_1235at 1448.237 kb on - strand, within Pf6N2E2_1235at 1448.419 kb on + strand, within Pf6N2E2_1235at 1448.419 kb on + strand, within Pf6N2E2_1235at 1448.458 kb on + strand, within Pf6N2E2_1235at 1448.507 kb on - strand, within Pf6N2E2_1235at 1448.565 kb on - strandat 1448.565 kb on - strandat 1448.664 kb on - strandat 1448.732 kb on - strandat 1448.840 kb on - strandat 1448.856 kb on - strandat 1448.856 kb on - strandat 1448.856 kb on - strandat 1448.868 kb on - strandat 1448.868 kb on - strandat 1448.925 kb on - strandat 1448.928 kb on + strandat 1448.929 kb on - strandat 1448.995 kb on + strandat 1449.163 kb on - strand, within Pf6N2E2_1236at 1449.425 kb on + strand, within Pf6N2E2_1236at 1449.425 kb on + strand, within Pf6N2E2_1236at 1449.426 kb on - strand, within Pf6N2E2_1236

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 4 mM
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1,446,445 + -0.9
1,446,703 - +2.5
1,446,703 - -0.6
1,446,703 - +0.3
1,446,779 + Pf6N2E2_1234 0.19 +1.4
1,446,782 + Pf6N2E2_1234 0.20 +2.8
1,446,782 + Pf6N2E2_1234 0.20 -0.2
1,447,383 - Pf6N2E2_1235 0.12 +0.8
1,447,825 + Pf6N2E2_1235 0.42 +2.1
1,447,825 + Pf6N2E2_1235 0.42 +1.0
1,447,826 - Pf6N2E2_1235 0.43 -0.7
1,447,865 - Pf6N2E2_1235 0.45 +0.1
1,447,865 - Pf6N2E2_1235 0.45 +2.1
1,447,912 + Pf6N2E2_1235 0.48 -0.5
1,447,950 - Pf6N2E2_1235 0.51 -0.7
1,447,966 + Pf6N2E2_1235 0.52 +1.8
1,447,966 + Pf6N2E2_1235 0.52 +0.9
1,447,966 + Pf6N2E2_1235 0.52 -0.1
1,447,967 - Pf6N2E2_1235 0.52 +1.0
1,447,981 + Pf6N2E2_1235 0.53 +0.6
1,448,002 + Pf6N2E2_1235 0.55 -0.3
1,448,002 + Pf6N2E2_1235 0.55 -2.1
1,448,003 - Pf6N2E2_1235 0.55 +0.6
1,448,003 - Pf6N2E2_1235 0.55 -4.2
1,448,017 + Pf6N2E2_1235 0.56 +1.6
1,448,017 + Pf6N2E2_1235 0.56 +1.3
1,448,017 + Pf6N2E2_1235 0.56 -0.2
1,448,017 + Pf6N2E2_1235 0.56 +1.2
1,448,044 + Pf6N2E2_1235 0.57 +2.1
1,448,107 + Pf6N2E2_1235 0.62 +1.0
1,448,107 + Pf6N2E2_1235 0.62 +0.2
1,448,107 + Pf6N2E2_1235 0.62 +0.9
1,448,108 - Pf6N2E2_1235 0.62 +0.8
1,448,140 + Pf6N2E2_1235 0.64 +1.7
1,448,140 + Pf6N2E2_1235 0.64 -0.9
1,448,140 + Pf6N2E2_1235 0.64 -2.2
1,448,140 + Pf6N2E2_1235 0.64 -1.0
1,448,158 + -0.5
1,448,200 + Pf6N2E2_1235 0.68 -3.2
1,448,236 + Pf6N2E2_1235 0.71 +0.3
1,448,236 + Pf6N2E2_1235 0.71 -1.6
1,448,236 + Pf6N2E2_1235 0.71 +1.3
1,448,236 + Pf6N2E2_1235 0.71 +0.4
1,448,237 - Pf6N2E2_1235 0.71 -0.2
1,448,237 - Pf6N2E2_1235 0.71 -0.2
1,448,237 - Pf6N2E2_1235 0.71 -0.5
1,448,237 - Pf6N2E2_1235 0.71 -1.8
1,448,419 + Pf6N2E2_1235 0.83 +1.9
1,448,419 + Pf6N2E2_1235 0.83 +4.3
1,448,458 + Pf6N2E2_1235 0.86 -0.6
1,448,507 - Pf6N2E2_1235 0.89 -0.3
1,448,565 - +0.5
1,448,565 - +1.6
1,448,664 - -3.2
1,448,732 - -0.9
1,448,840 - +0.7
1,448,856 - -0.6
1,448,856 - -1.9
1,448,856 - +0.1
1,448,868 - -0.5
1,448,868 - +0.9
1,448,925 - +1.0
1,448,928 + +0.5
1,448,929 - +0.3
1,448,995 + -0.5
1,449,163 - Pf6N2E2_1236 0.18 +0.4
1,449,425 + Pf6N2E2_1236 0.47 -0.8
1,449,425 + Pf6N2E2_1236 0.47 +1.2
1,449,426 - Pf6N2E2_1236 0.47 +0.9

Or see this region's nucleotide sequence