Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_867

Experiment: Thallium(I) acetate 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_865 and Pf1N1B4_866 are separated by 50 nucleotidesPf1N1B4_866 and Pf1N1B4_867 are separated by 295 nucleotidesPf1N1B4_867 and Pf1N1B4_868 are separated by 2 nucleotidesPf1N1B4_868 and Pf1N1B4_869 are separated by 146 nucleotides Pf1N1B4_865 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 992,119 to 993,333 _865 Pf1N1B4_866 - Iron-sulfur cluster regulator IscR, at 993,384 to 993,845 _866 Pf1N1B4_867 - Serine acetyltransferase (EC 2.3.1.30), at 994,141 to 994,917 _867 Pf1N1B4_868 - tRNA:Cm32/Um32 methyltransferase, at 994,920 to 995,690 _868 Pf1N1B4_869 - Inositol-1-monophosphatase (EC 3.1.3.25), at 995,837 to 996,655 _869 Position (kb) 994 995Strain fitness (log2 ratio) -2 -1 0 1 2 3at 993.346 kb on - strandat 993.346 kb on - strandat 993.346 kb on - strandat 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.884 kb on - strandat 993.884 kb on - strandat 993.884 kb on - strandat 993.884 kb on - strandat 993.990 kb on - strandat 994.007 kb on + strandat 994.007 kb on + strandat 994.007 kb on + strandat 994.142 kb on + strandat 994.171 kb on + strandat 994.172 kb on - strandat 994.220 kb on + strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.273 kb on + strand, within Pf1N1B4_867at 994.936 kb on - strandat 994.936 kb on - strandat 994.986 kb on + strandat 994.986 kb on + strandat 994.986 kb on + strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.987 kb on - strandat 994.988 kb on + strandat 994.988 kb on + strandat 994.989 kb on - strandat 995.024 kb on - strand, within Pf1N1B4_868at 995.110 kb on + strand, within Pf1N1B4_868at 995.111 kb on - strandat 995.111 kb on - strand, within Pf1N1B4_868at 995.216 kb on + strand, within Pf1N1B4_868at 995.216 kb on + strand, within Pf1N1B4_868at 995.216 kb on + strand, within Pf1N1B4_868at 995.216 kb on + strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.217 kb on - strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.227 kb on + strand, within Pf1N1B4_868at 995.228 kb on - strand, within Pf1N1B4_868at 995.228 kb on - strand, within Pf1N1B4_868at 995.228 kb on - strand, within Pf1N1B4_868at 995.228 kb on - strand, within Pf1N1B4_868at 995.228 kb on - strand, within Pf1N1B4_868at 995.299 kb on + strand, within Pf1N1B4_868at 995.300 kb on - strand, within Pf1N1B4_868at 995.388 kb on + strand, within Pf1N1B4_868at 995.389 kb on - strand, within Pf1N1B4_868at 995.730 kb on + strandat 995.742 kb on + strandat 995.742 kb on + strandat 995.753 kb on + strandat 995.753 kb on + strandat 995.753 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml
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993,346 - +2.1
993,346 - -0.3
993,346 - +1.7
993,487 - Pf1N1B4_866 0.22 +2.7
993,487 - Pf1N1B4_866 0.22 +2.9
993,487 - Pf1N1B4_866 0.22 +1.1
993,487 - Pf1N1B4_866 0.22 +1.8
993,884 - +1.6
993,884 - +2.1
993,884 - -0.7
993,884 - +0.4
993,990 - -0.3
994,007 + -1.3
994,007 + -0.9
994,007 + +0.8
994,142 + +0.1
994,171 + +0.1
994,172 - -0.9
994,220 + Pf1N1B4_867 0.10 +1.5
994,221 - Pf1N1B4_867 0.10 -1.1
994,221 - Pf1N1B4_867 0.10 -0.5
994,221 - Pf1N1B4_867 0.10 -1.5
994,221 - Pf1N1B4_867 0.10 -0.8
994,273 + Pf1N1B4_867 0.17 +1.7
994,936 - +1.1
994,936 - +0.1
994,986 + +0.1
994,986 + +2.7
994,986 + +1.1
994,987 - -1.3
994,987 - +2.1
994,987 - -0.5
994,987 - -1.7
994,987 - -1.5
994,987 - +0.7
994,987 - -1.3
994,987 - +0.5
994,987 - +0.7
994,987 - -1.5
994,987 - +0.5
994,987 - +0.1
994,987 - -1.6
994,988 + +1.4
994,988 + -0.9
994,989 - +0.4
995,024 - Pf1N1B4_868 0.13 -1.5
995,110 + Pf1N1B4_868 0.25 +0.8
995,111 - +0.1
995,111 - Pf1N1B4_868 0.25 -0.9
995,216 + Pf1N1B4_868 0.38 +0.1
995,216 + Pf1N1B4_868 0.38 +1.8
995,216 + Pf1N1B4_868 0.38 -0.9
995,216 + Pf1N1B4_868 0.38 +0.3
995,217 - Pf1N1B4_868 0.39 -0.9
995,217 - Pf1N1B4_868 0.39 +1.1
995,217 - Pf1N1B4_868 0.39 -0.9
995,217 - Pf1N1B4_868 0.39 -0.5
995,217 - Pf1N1B4_868 0.39 -1.5
995,217 - Pf1N1B4_868 0.39 -0.4
995,217 - Pf1N1B4_868 0.39 -0.9
995,227 + Pf1N1B4_868 0.40 +0.4
995,227 + Pf1N1B4_868 0.40 -0.7
995,227 + Pf1N1B4_868 0.40 -1.3
995,227 + Pf1N1B4_868 0.40 +1.4
995,227 + Pf1N1B4_868 0.40 -2.5
995,227 + Pf1N1B4_868 0.40 -0.9
995,228 - Pf1N1B4_868 0.40 -0.7
995,228 - Pf1N1B4_868 0.40 -1.7
995,228 - Pf1N1B4_868 0.40 -1.9
995,228 - Pf1N1B4_868 0.40 +0.1
995,228 - Pf1N1B4_868 0.40 -0.9
995,299 + Pf1N1B4_868 0.49 +0.3
995,300 - Pf1N1B4_868 0.49 +0.8
995,388 + Pf1N1B4_868 0.61 +0.6
995,389 - Pf1N1B4_868 0.61 -0.7
995,730 + +0.1
995,742 + +0.5
995,742 + -0.2
995,753 + +0.4
995,753 + -0.3
995,753 + -0.7

Or see this region's nucleotide sequence