Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_65

Experiment: Thallium(I) acetate 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotidesPf1N1B4_64 and Pf1N1B4_65 are separated by 194 nucleotidesPf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotidesPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357 _63 Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730 _64 Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755 _65 Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Position (kb) 67 68 69Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 66.991 kb on + strandat 66.992 kb on - strandat 66.992 kb on - strand, within Pf1N1B4_63at 67.322 kb on - strandat 67.390 kb on - strandat 67.394 kb on + strandat 67.394 kb on + strandat 67.395 kb on - strandat 67.395 kb on - strandat 67.408 kb on - strandat 67.408 kb on - strandat 67.433 kb on + strandat 67.434 kb on - strandat 67.451 kb on + strandat 67.741 kb on + strandat 67.741 kb on + strandat 67.838 kb on + strandat 67.838 kb on + strandat 67.839 kb on - strandat 67.839 kb on - strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.927 kb on - strandat 67.927 kb on - strandat 68.011 kb on - strand, within Pf1N1B4_65at 68.011 kb on - strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.232 kb on - strand, within Pf1N1B4_65at 68.245 kb on + strand, within Pf1N1B4_65at 68.246 kb on - strandat 68.292 kb on + strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.484 kb on + strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strandat 68.620 kb on - strand, within Pf1N1B4_65at 68.620 kb on - strand, within Pf1N1B4_65at 68.698 kb on - strandat 68.698 kb on - strandat 68.709 kb on + strandat 68.710 kb on - strandat 68.871 kb on - strand, within Pf1N1B4_66at 69.038 kb on + strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strandat 69.045 kb on - strand, within Pf1N1B4_66at 69.129 kb on + strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strandat 69.150 kb on + strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67

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Per-strain Table

Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml
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66,991 + -1.3
66,992 - +0.2
66,992 - Pf1N1B4_63 0.41 -0.6
67,322 - +0.7
67,390 - -0.3
67,394 + +0.7
67,394 + -0.8
67,395 - -2.3
67,395 - -0.3
67,408 - -0.7
67,408 - +0.1
67,433 + +2.3
67,434 - -0.3
67,451 + -1.3
67,741 + +0.1
67,741 + +1.1
67,838 + +1.6
67,838 + -0.5
67,839 - -0.3
67,839 - +0.6
67,923 + +0.3
67,923 + -0.3
67,923 + +1.2
67,924 - +1.1
67,924 - +0.8
67,924 - +0.4
67,924 - -1.4
67,924 - +0.3
67,924 - -1.6
67,924 - -0.8
67,924 - -0.3
67,927 - +2.3
67,927 - +2.0
68,011 - Pf1N1B4_65 0.10 -1.3
68,011 - Pf1N1B4_65 0.10 -1.1
68,019 + Pf1N1B4_65 0.11 -0.8
68,019 + Pf1N1B4_65 0.11 -1.3
68,019 + Pf1N1B4_65 0.11 -2.1
68,020 - Pf1N1B4_65 0.11 -0.8
68,020 - Pf1N1B4_65 0.11 -1.3
68,020 - Pf1N1B4_65 0.11 -2.8
68,020 - Pf1N1B4_65 0.11 -1.3
68,122 - Pf1N1B4_65 0.24 -1.3
68,122 - Pf1N1B4_65 0.24 -0.5
68,232 - Pf1N1B4_65 0.37 +1.0
68,245 + Pf1N1B4_65 0.39 -1.8
68,246 - -0.8
68,292 + Pf1N1B4_65 0.44 -0.3
68,293 - Pf1N1B4_65 0.44 -1.3
68,293 - Pf1N1B4_65 0.44 -0.3
68,293 - Pf1N1B4_65 0.44 -0.8
68,293 - Pf1N1B4_65 0.44 -1.4
68,293 - Pf1N1B4_65 0.44 +1.3
68,293 - Pf1N1B4_65 0.44 +1.1
68,484 + Pf1N1B4_65 0.67 -2.8
68,485 - Pf1N1B4_65 0.67 -0.0
68,485 - Pf1N1B4_65 0.67 +1.6
68,485 - Pf1N1B4_65 0.67 -0.7
68,619 + Pf1N1B4_65 0.84 -0.6
68,619 + Pf1N1B4_65 0.84 +0.0
68,619 + -0.8
68,620 - Pf1N1B4_65 0.84 -1.3
68,620 - Pf1N1B4_65 0.84 -0.3
68,698 - +1.6
68,698 - -2.8
68,709 + -1.5
68,710 - -0.0
68,871 - Pf1N1B4_66 0.10 -0.3
69,038 + Pf1N1B4_66 0.41 -0.3
69,045 - Pf1N1B4_66 0.42 -0.4
69,045 - Pf1N1B4_66 0.42 +0.6
69,045 - Pf1N1B4_66 0.42 -0.5
69,045 - Pf1N1B4_66 0.42 -0.5
69,045 - -0.3
69,045 - Pf1N1B4_66 0.42 +0.2
69,129 + Pf1N1B4_66 0.58 -0.7
69,130 - Pf1N1B4_66 0.58 -0.6
69,130 - Pf1N1B4_66 0.58 -0.3
69,141 - Pf1N1B4_66 0.60 +0.9
69,141 - Pf1N1B4_66 0.60 +1.3
69,141 - Pf1N1B4_66 0.60 +0.5
69,141 - Pf1N1B4_66 0.60 +1.1
69,141 - -0.8
69,150 + Pf1N1B4_66 0.62 -0.3
69,207 - Pf1N1B4_66 0.73 -1.3
69,207 - Pf1N1B4_66 0.73 +0.3
69,271 + Pf1N1B4_66 0.85 -1.3
69,330 + -1.3
69,330 + +1.3
69,395 + -1.8
69,432 + +0.6
69,432 + +3.1
69,433 - -0.5
69,433 - +0.3
69,433 - -0.3
69,433 - -1.1
69,473 + +0.3
69,474 - +0.5
69,474 - +1.6
69,476 - +1.2
69,476 - -0.8
69,639 - Pf1N1B4_67 0.20 -0.3
69,729 + Pf1N1B4_67 0.33 -0.6

Or see this region's nucleotide sequence