Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotides Pf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotides Pf1N1B4_5242 and Pf1N1B4_5243 are separated by 71 nucleotides Pf1N1B4_5243 and Pf1N1B4_5244 are separated by 85 nucleotides Pf1N1B4_5244 and Pf1N1B4_5245 are separated by 42 nucleotides
Pf1N1B4_5240 - hypothetical protein, at 753 to 872
_5240
Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271
_5241
Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524
_5242
Pf1N1B4_5243 - hypothetical protein, at 1,596 to 1,763
_5243
Pf1N1B4_5244 - hypothetical protein, at 1,849 to 2,097
_5244
Pf1N1B4_5245 - hypothetical protein, at 2,140 to 2,784
_5245
Position (kb)
1
2 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 0.597 kb on - strand at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.943 kb on + strand at 0.943 kb on + strand at 0.992 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.075 kb on - strand at 1.075 kb on - strand at 1.092 kb on - strand, within Pf1N1B4_5241 at 1.265 kb on + strand at 1.265 kb on + strand at 1.337 kb on - strand, within Pf1N1B4_5242 at 1.369 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.501 kb on + strand at 1.501 kb on + strand at 1.664 kb on + strand, within Pf1N1B4_5243 at 1.664 kb on + strand, within Pf1N1B4_5243 at 1.665 kb on - strand, within Pf1N1B4_5243 at 1.689 kb on + strand at 1.786 kb on + strand at 1.787 kb on - strand at 1.953 kb on + strand, within Pf1N1B4_5244 at 1.954 kb on - strand, within Pf1N1B4_5244 at 2.026 kb on + strand, within Pf1N1B4_5244 at 2.042 kb on + strand, within Pf1N1B4_5244 at 2.096 kb on - strand at 2.096 kb on - strand at 2.239 kb on - strand, within Pf1N1B4_5245 at 2.240 kb on + strand at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.265 kb on - strand, within Pf1N1B4_5245 at 2.265 kb on - strand, within Pf1N1B4_5245 at 2.324 kb on - strand, within Pf1N1B4_5245 at 2.346 kb on - strand, within Pf1N1B4_5245 at 2.370 kb on + strand, within Pf1N1B4_5245 at 2.588 kb on + strand, within Pf1N1B4_5245 at 2.588 kb on + strand at 2.589 kb on - strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.649 kb on - strand, within Pf1N1B4_5245 at 2.649 kb on - strand, within Pf1N1B4_5245 at 2.678 kb on + strand, within Pf1N1B4_5245 at 2.679 kb on - strand, within Pf1N1B4_5245 at 2.679 kb on - strand, within Pf1N1B4_5245
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 597 - -0.8 773 - Pf1N1B4_5240 0.17 +0.7 773 - Pf1N1B4_5240 0.17 +1.3 773 - Pf1N1B4_5240 0.17 +0.4 943 + -0.9 943 + +1.5 992 + +0.8 1,074 + +1.1 1,074 + -2.5 1,074 + +0.5 1,074 + -1.5 1,074 + -1.5 1,075 - -1.2 1,075 - +1.9 1,092 - Pf1N1B4_5241 0.10 +0.1 1,265 + -0.9 1,265 + +0.8 1,337 - Pf1N1B4_5242 0.28 +0.1 1,369 + Pf1N1B4_5242 0.40 -0.9 1,407 + Pf1N1B4_5242 0.55 +1.1 1,407 + Pf1N1B4_5242 0.55 +0.7 1,501 + -1.6 1,501 + +1.1 1,664 + Pf1N1B4_5243 0.40 -1.5 1,664 + Pf1N1B4_5243 0.40 -0.5 1,665 - Pf1N1B4_5243 0.41 -0.8 1,689 + +1.7 1,786 + -0.3 1,787 - -0.5 1,953 + Pf1N1B4_5244 0.42 -0.9 1,954 - Pf1N1B4_5244 0.42 +0.5 2,026 + Pf1N1B4_5244 0.71 +0.6 2,042 + Pf1N1B4_5244 0.78 -0.3 2,096 - +0.4 2,096 - +0.7 2,239 - Pf1N1B4_5245 0.15 +0.1 2,240 + +1.7 2,241 - Pf1N1B4_5245 0.16 +0.1 2,241 - Pf1N1B4_5245 0.16 +0.8 2,241 - Pf1N1B4_5245 0.16 +1.4 2,241 - Pf1N1B4_5245 0.16 -0.1 2,241 - Pf1N1B4_5245 0.16 +0.3 2,241 - Pf1N1B4_5245 0.16 +0.5 2,241 - Pf1N1B4_5245 0.16 +0.1 2,265 - Pf1N1B4_5245 0.19 +1.7 2,265 - Pf1N1B4_5245 0.19 +0.4 2,324 - Pf1N1B4_5245 0.29 -0.4 2,346 - Pf1N1B4_5245 0.32 -3.1 2,370 + Pf1N1B4_5245 0.36 -1.9 2,588 + Pf1N1B4_5245 0.69 +0.9 2,588 + +0.1 2,589 - Pf1N1B4_5245 0.70 +0.1 2,648 + Pf1N1B4_5245 0.79 -0.9 2,648 + Pf1N1B4_5245 0.79 -0.3 2,648 + +1.3 2,648 + Pf1N1B4_5245 0.79 +1.1 2,648 + Pf1N1B4_5245 0.79 +1.1 2,648 + Pf1N1B4_5245 0.79 -0.3 2,648 + Pf1N1B4_5245 0.79 +0.2 2,649 - Pf1N1B4_5245 0.79 -0.3 2,649 - Pf1N1B4_5245 0.79 +0.5 2,678 + Pf1N1B4_5245 0.83 +1.5 2,679 - Pf1N1B4_5245 0.84 +3.4 2,679 - Pf1N1B4_5245 0.84 +1.1
Or see this region's nucleotide sequence