Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4192 and Pf1N1B4_4193 are separated by 47 nucleotides Pf1N1B4_4193 and Pf1N1B4_4194 are separated by 52 nucleotides Pf1N1B4_4194 and Pf1N1B4_4195 are separated by 146 nucleotides Pf1N1B4_4195 and Pf1N1B4_4196 overlap by 37 nucleotides Pf1N1B4_4196 and Pf1N1B4_4197 overlap by 2 nucleotides
Pf1N1B4_4192 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,644,910 to 4,645,776
_4192
Pf1N1B4_4193 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,645,824 to 4,646,612
_4193
Pf1N1B4_4194 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,646,665 to 4,647,786
_4194
Pf1N1B4_4195 - Transcriptional regulator, TetR family, at 4,647,933 to 4,648,505
_4195
Pf1N1B4_4196 - hypothetical protein, at 4,648,469 to 4,648,630
_4196
Pf1N1B4_4197 - Glucose ABC transport system, periplasmic sugar-binding protein, at 4,648,629 to 4,648,754
_4197
Position (kb) 4646
4647
4648 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4645.762 kb on - strand at 4645.841 kb on - strand at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.166 kb on - strand, within Pf1N1B4_4193 at 4646.598 kb on - strand at 4646.732 kb on + strand at 4646.733 kb on - strand at 4646.733 kb on - strand at 4646.753 kb on + strand at 4646.869 kb on - strand at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand, within Pf1N1B4_4194 at 4646.915 kb on + strand at 4646.916 kb on - strand, within Pf1N1B4_4194 at 4646.916 kb on - strand at 4646.916 kb on - strand at 4646.916 kb on - strand, within Pf1N1B4_4194 at 4647.246 kb on - strand, within Pf1N1B4_4194 at 4647.563 kb on + strand at 4647.657 kb on - strand, within Pf1N1B4_4194 at 4647.766 kb on - strand at 4647.821 kb on - strand at 4647.886 kb on + strand at 4647.887 kb on - strand at 4647.890 kb on + strand at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.329 kb on + strand, within Pf1N1B4_4195
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 4,645,762 - -1.5 4,645,841 - -0.4 4,646,083 + Pf1N1B4_4193 0.33 -0.6 4,646,083 + Pf1N1B4_4193 0.33 +0.0 4,646,083 + -2.0 4,646,084 - Pf1N1B4_4193 0.33 -0.5 4,646,084 - Pf1N1B4_4193 0.33 -1.1 4,646,084 - Pf1N1B4_4193 0.33 -2.0 4,646,084 - Pf1N1B4_4193 0.33 -0.8 4,646,166 - Pf1N1B4_4193 0.43 -1.4 4,646,598 - -0.7 4,646,732 + -1.4 4,646,733 - -0.0 4,646,733 - +0.0 4,646,753 + +1.0 4,646,869 - +0.2 4,646,915 + Pf1N1B4_4194 0.22 -1.2 4,646,915 + Pf1N1B4_4194 0.22 -2.7 4,646,915 + Pf1N1B4_4194 0.22 -0.8 4,646,915 + Pf1N1B4_4194 0.22 -2.2 4,646,915 + +1.2 4,646,916 - Pf1N1B4_4194 0.22 -0.4 4,646,916 - -1.0 4,646,916 - +1.2 4,646,916 - Pf1N1B4_4194 0.22 -0.0 4,647,246 - Pf1N1B4_4194 0.52 +2.4 4,647,563 + -0.4 4,647,657 - Pf1N1B4_4194 0.88 -0.5 4,647,766 - +0.9 4,647,821 - +1.1 4,647,886 + +1.8 4,647,887 - -2.7 4,647,890 + -0.4 4,648,185 + -0.8 4,648,185 + Pf1N1B4_4195 0.44 -0.1 4,648,185 + Pf1N1B4_4195 0.44 -1.4 4,648,185 + Pf1N1B4_4195 0.44 +1.6 4,648,185 + Pf1N1B4_4195 0.44 -2.2 4,648,185 + Pf1N1B4_4195 0.44 +0.4 4,648,185 + Pf1N1B4_4195 0.44 -1.4 4,648,185 + -0.4 4,648,185 + Pf1N1B4_4195 0.44 -2.4 4,648,185 + +0.0 4,648,186 - Pf1N1B4_4195 0.44 +1.5 4,648,186 - Pf1N1B4_4195 0.44 +1.0 4,648,186 - Pf1N1B4_4195 0.44 -1.4 4,648,186 - -2.4 4,648,186 - Pf1N1B4_4195 0.44 +1.8 4,648,186 - Pf1N1B4_4195 0.44 -0.4 4,648,329 + Pf1N1B4_4195 0.69 -2.0
Or see this region's nucleotide sequence