Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3234 and Pf1N1B4_3235 are separated by 51 nucleotides Pf1N1B4_3235 and Pf1N1B4_3236 are separated by 1 nucleotides Pf1N1B4_3236 and Pf1N1B4_3237 are separated by 157 nucleotides
Pf1N1B4_3234 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,620,885 to 3,622,087
_3234
Pf1N1B4_3235 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,622,139 to 3,622,843
_3235
Pf1N1B4_3236 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,622,845 to 3,623,411
_3236
Pf1N1B4_3237 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,623,569 to 3,624,933
_3237
Position (kb)
3622
3623 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3621.726 kb on + strand at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.727 kb on - strand, within Pf1N1B4_3234 at 3621.982 kb on - strand at 3622.104 kb on + strand at 3622.105 kb on - strand at 3622.116 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.120 kb on - strand at 3622.138 kb on - strand at 3622.138 kb on - strand at 3622.190 kb on + strand at 3622.190 kb on + strand at 3622.191 kb on - strand at 3622.206 kb on - strand at 3622.365 kb on + strand at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.703 kb on + strand, within Pf1N1B4_3235 at 3622.778 kb on + strand at 3622.779 kb on - strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.884 kb on + strand at 3622.885 kb on - strand at 3622.885 kb on - strand at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.139 kb on - strand, within Pf1N1B4_3236 at 3623.250 kb on + strand, within Pf1N1B4_3236 at 3623.250 kb on + strand, within Pf1N1B4_3236 at 3623.250 kb on + strand, within Pf1N1B4_3236 at 3623.352 kb on + strand, within Pf1N1B4_3236 at 3623.353 kb on - strand, within Pf1N1B4_3236 at 3623.353 kb on - strand, within Pf1N1B4_3236 at 3623.568 kb on + strand at 3623.568 kb on + strand at 3623.568 kb on + strand at 3623.605 kb on + strand at 3623.605 kb on + strand at 3623.605 kb on + strand at 3623.606 kb on - strand at 3623.606 kb on - strand at 3623.606 kb on - strand at 3623.764 kb on + strand, within Pf1N1B4_3237
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 3,621,726 + +0.7 3,621,726 + Pf1N1B4_3234 0.70 +0.3 3,621,726 + Pf1N1B4_3234 0.70 -0.1 3,621,726 + Pf1N1B4_3234 0.70 -1.9 3,621,727 - Pf1N1B4_3234 0.70 -0.1 3,621,982 - -1.6 3,622,104 + -3.1 3,622,105 - +0.3 3,622,116 + -0.1 3,622,119 + -1.6 3,622,119 + +1.9 3,622,119 + -0.5 3,622,120 - -1.6 3,622,138 - -0.1 3,622,138 - -1.6 3,622,190 + +0.5 3,622,190 + -0.5 3,622,191 - +0.9 3,622,206 - +0.3 3,622,365 + -0.1 3,622,365 + Pf1N1B4_3235 0.32 +0.4 3,622,365 + Pf1N1B4_3235 0.32 -0.6 3,622,366 - Pf1N1B4_3235 0.32 +1.7 3,622,366 - Pf1N1B4_3235 0.32 -1.1 3,622,366 - Pf1N1B4_3235 0.32 +0.4 3,622,664 + Pf1N1B4_3235 0.74 +0.9 3,622,664 + Pf1N1B4_3235 0.74 +0.9 3,622,664 + Pf1N1B4_3235 0.74 -1.1 3,622,664 + Pf1N1B4_3235 0.74 +1.5 3,622,665 - Pf1N1B4_3235 0.75 -1.1 3,622,665 - Pf1N1B4_3235 0.75 +0.7 3,622,665 - Pf1N1B4_3235 0.75 +0.7 3,622,665 - Pf1N1B4_3235 0.75 +0.9 3,622,665 - Pf1N1B4_3235 0.75 -0.1 3,622,665 - Pf1N1B4_3235 0.75 +0.5 3,622,692 - Pf1N1B4_3235 0.78 -1.6 3,622,692 - Pf1N1B4_3235 0.78 +2.4 3,622,703 + Pf1N1B4_3235 0.80 +2.8 3,622,778 + -0.6 3,622,779 - +1.1 3,622,841 + -0.3 3,622,841 + +0.8 3,622,841 + -1.1 3,622,841 + -0.6 3,622,841 + +2.5 3,622,841 + -1.3 3,622,842 - -1.1 3,622,842 - -0.1 3,622,842 - -1.6 3,622,884 + +0.5 3,622,885 - +2.3 3,622,885 - -0.1 3,623,043 + Pf1N1B4_3236 0.35 +0.4 3,623,043 + Pf1N1B4_3236 0.35 +0.5 3,623,043 + Pf1N1B4_3236 0.35 -0.5 3,623,043 + Pf1N1B4_3236 0.35 -0.4 3,623,044 - Pf1N1B4_3236 0.35 -0.5 3,623,044 - Pf1N1B4_3236 0.35 -0.1 3,623,044 - Pf1N1B4_3236 0.35 +0.9 3,623,139 - Pf1N1B4_3236 0.52 -1.9 3,623,250 + Pf1N1B4_3236 0.71 +0.4 3,623,250 + Pf1N1B4_3236 0.71 +1.3 3,623,250 + Pf1N1B4_3236 0.71 +0.9 3,623,352 + Pf1N1B4_3236 0.89 +0.2 3,623,353 - Pf1N1B4_3236 0.90 -0.1 3,623,353 - Pf1N1B4_3236 0.90 -0.1 3,623,568 + +0.9 3,623,568 + +0.9 3,623,568 + -0.3 3,623,605 + -0.6 3,623,605 + -1.1 3,623,605 + -2.6 3,623,606 - -0.1 3,623,606 - -0.1 3,623,606 - -0.2 3,623,764 + Pf1N1B4_3237 0.14 -0.7
Or see this region's nucleotide sequence