Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1864 and Pf1N1B4_1865 are separated by 60 nucleotides Pf1N1B4_1865 and Pf1N1B4_1866 overlap by 1 nucleotides
Pf1N1B4_1864 - Methionine ABC transporter substrate-binding protein, at 2,133,652 to 2,134,425
_1864
Pf1N1B4_1865 - Methionine ABC transporter permease protein, at 2,134,486 to 2,135,160
_1865
Pf1N1B4_1866 - Methionine ABC transporter ATP-binding protein, at 2,135,160 to 2,136,167
_1866
Position (kb)
2134
2135
2136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2133.504 kb on + strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.539 kb on + strand at 2133.540 kb on - strand at 2133.540 kb on - strand at 2133.540 kb on - strand at 2133.544 kb on + strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.555 kb on + strand at 2133.556 kb on - strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.681 kb on - strand at 2133.681 kb on - strand at 2133.788 kb on + strand, within Pf1N1B4_1864 at 2133.788 kb on + strand, within Pf1N1B4_1864 at 2133.789 kb on - strand, within Pf1N1B4_1864 at 2133.789 kb on - strand, within Pf1N1B4_1864 at 2133.854 kb on + strand, within Pf1N1B4_1864 at 2133.854 kb on + strand, within Pf1N1B4_1864 at 2133.855 kb on - strand, within Pf1N1B4_1864 at 2133.855 kb on - strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.112 kb on + strand, within Pf1N1B4_1864 at 2134.112 kb on + strand, within Pf1N1B4_1864 at 2134.113 kb on - strand, within Pf1N1B4_1864 at 2134.191 kb on - strand, within Pf1N1B4_1864 at 2134.256 kb on + strand, within Pf1N1B4_1864 at 2134.256 kb on + strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.414 kb on + strand at 2134.432 kb on - strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.661 kb on + strand, within Pf1N1B4_1865 at 2134.662 kb on - strand, within Pf1N1B4_1865 at 2134.662 kb on - strand, within Pf1N1B4_1865 at 2134.674 kb on + strand, within Pf1N1B4_1865 at 2134.674 kb on + strand, within Pf1N1B4_1865 at 2134.675 kb on - strand, within Pf1N1B4_1865 at 2134.675 kb on - strand, within Pf1N1B4_1865 at 2134.675 kb on - strand at 2134.689 kb on + strand, within Pf1N1B4_1865 at 2134.689 kb on + strand, within Pf1N1B4_1865 at 2134.690 kb on - strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.982 kb on + strand, within Pf1N1B4_1865 at 2134.983 kb on - strand, within Pf1N1B4_1865 at 2135.065 kb on + strand, within Pf1N1B4_1865 at 2135.065 kb on + strand, within Pf1N1B4_1865 at 2135.066 kb on - strand, within Pf1N1B4_1865 at 2135.095 kb on - strand at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.300 kb on - strand, within Pf1N1B4_1866 at 2135.344 kb on + strand, within Pf1N1B4_1866 at 2136.032 kb on + strand, within Pf1N1B4_1866 at 2136.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 2,133,504 + +1.2 2,133,505 - +2.1 2,133,505 - +0.5 2,133,505 - +0.5 2,133,505 - -2.1 2,133,505 - +0.5 2,133,539 + -0.2 2,133,540 - -0.3 2,133,540 - -0.5 2,133,540 - -1.8 2,133,544 + -0.2 2,133,545 - +1.3 2,133,545 - +0.2 2,133,545 - -1.5 2,133,545 - +0.5 2,133,545 - -1.5 2,133,555 + -0.9 2,133,556 - +0.7 2,133,680 + -0.5 2,133,680 + +0.2 2,133,680 + -0.3 2,133,680 + -1.1 2,133,680 + +0.7 2,133,680 + +0.5 2,133,680 + +0.5 2,133,680 + -2.1 2,133,681 - +0.5 2,133,681 - -1.8 2,133,788 + Pf1N1B4_1864 0.18 +0.1 2,133,788 + Pf1N1B4_1864 0.18 +1.5 2,133,789 - Pf1N1B4_1864 0.18 -1.8 2,133,789 - Pf1N1B4_1864 0.18 +0.1 2,133,854 + Pf1N1B4_1864 0.26 -0.3 2,133,854 + Pf1N1B4_1864 0.26 -0.5 2,133,855 - Pf1N1B4_1864 0.26 -0.5 2,133,855 - Pf1N1B4_1864 0.26 +0.5 2,134,100 + Pf1N1B4_1864 0.58 -0.0 2,134,100 + Pf1N1B4_1864 0.58 +1.2 2,134,100 + Pf1N1B4_1864 0.58 -2.5 2,134,100 + Pf1N1B4_1864 0.58 +0.1 2,134,100 + Pf1N1B4_1864 0.58 +0.9 2,134,100 + Pf1N1B4_1864 0.58 +0.8 2,134,101 - Pf1N1B4_1864 0.58 -1.1 2,134,101 - Pf1N1B4_1864 0.58 +0.5 2,134,101 - Pf1N1B4_1864 0.58 +0.7 2,134,101 - Pf1N1B4_1864 0.58 +0.3 2,134,101 - Pf1N1B4_1864 0.58 -0.8 2,134,101 - Pf1N1B4_1864 0.58 -1.1 2,134,101 - Pf1N1B4_1864 0.58 +0.3 2,134,101 - Pf1N1B4_1864 0.58 +0.7 2,134,101 - Pf1N1B4_1864 0.58 -0.8 2,134,112 + Pf1N1B4_1864 0.59 +1.1 2,134,112 + Pf1N1B4_1864 0.59 -0.7 2,134,113 - Pf1N1B4_1864 0.60 +0.7 2,134,191 - Pf1N1B4_1864 0.70 -0.2 2,134,256 + Pf1N1B4_1864 0.78 +1.5 2,134,256 + Pf1N1B4_1864 0.78 -1.3 2,134,257 - Pf1N1B4_1864 0.78 +0.5 2,134,257 - Pf1N1B4_1864 0.78 -0.3 2,134,257 - Pf1N1B4_1864 0.78 -0.2 2,134,257 - Pf1N1B4_1864 0.78 +0.5 2,134,257 - Pf1N1B4_1864 0.78 -1.4 2,134,257 - Pf1N1B4_1864 0.78 -0.1 2,134,414 + +0.8 2,134,432 - +0.6 2,134,487 + -0.2 2,134,487 + -0.8 2,134,487 + +0.1 2,134,487 + -0.3 2,134,487 + +1.5 2,134,487 + +1.1 2,134,487 + +0.3 2,134,487 + -0.5 2,134,488 - +0.3 2,134,488 - -1.8 2,134,488 - +0.5 2,134,488 - +2.3 2,134,488 - -0.5 2,134,488 - -1.5 2,134,488 - +0.6 2,134,488 - +0.5 2,134,488 - +0.5 2,134,491 + +0.9 2,134,491 + -0.1 2,134,491 + +0.6 2,134,491 + +0.5 2,134,491 + -1.1 2,134,491 + +0.5 2,134,491 + +0.4 2,134,491 + -1.1 2,134,491 + -1.4 2,134,492 - -0.1 2,134,492 - +1.1 2,134,492 - +0.5 2,134,492 - -0.2 2,134,492 - +1.5 2,134,492 - -0.8 2,134,492 - -1.1 2,134,492 - -0.3 2,134,492 - -1.1 2,134,661 + Pf1N1B4_1865 0.26 -2.5 2,134,662 - Pf1N1B4_1865 0.26 -0.8 2,134,662 - Pf1N1B4_1865 0.26 +0.1 2,134,674 + Pf1N1B4_1865 0.28 -0.5 2,134,674 + Pf1N1B4_1865 0.28 -0.4 2,134,675 - Pf1N1B4_1865 0.28 +0.5 2,134,675 - Pf1N1B4_1865 0.28 +0.2 2,134,675 - +0.5 2,134,689 + Pf1N1B4_1865 0.30 -1.5 2,134,689 + Pf1N1B4_1865 0.30 -1.7 2,134,690 - Pf1N1B4_1865 0.30 -1.3 2,134,932 + Pf1N1B4_1865 0.66 -1.5 2,134,932 + Pf1N1B4_1865 0.66 -2.8 2,134,932 + Pf1N1B4_1865 0.66 -1.5 2,134,932 + Pf1N1B4_1865 0.66 -0.9 2,134,933 - Pf1N1B4_1865 0.66 -0.3 2,134,933 - Pf1N1B4_1865 0.66 -0.6 2,134,933 - Pf1N1B4_1865 0.66 -1.5 2,134,982 + Pf1N1B4_1865 0.73 +0.5 2,134,983 - Pf1N1B4_1865 0.74 +0.5 2,135,065 + Pf1N1B4_1865 0.86 -2.8 2,135,065 + Pf1N1B4_1865 0.86 -1.1 2,135,066 - Pf1N1B4_1865 0.86 -0.3 2,135,095 - -0.1 2,135,299 + Pf1N1B4_1866 0.14 -0.9 2,135,299 + Pf1N1B4_1866 0.14 -0.5 2,135,299 + Pf1N1B4_1866 0.14 +1.0 2,135,299 + Pf1N1B4_1866 0.14 -1.5 2,135,299 + Pf1N1B4_1866 0.14 -0.3 2,135,300 - Pf1N1B4_1866 0.14 -1.3 2,135,344 + Pf1N1B4_1866 0.18 -0.7 2,136,032 + Pf1N1B4_1866 0.87 -2.7 2,136,117 - +0.5
Or see this region's nucleotide sequence