Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1144 and Pf1N1B4_1145 are separated by 11 nucleotides Pf1N1B4_1145 and Pf1N1B4_1146 overlap by 1 nucleotides Pf1N1B4_1146 and Pf1N1B4_1147 are separated by 301 nucleotides
Pf1N1B4_1144 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 1,286,781 to 1,288,520
_1144
Pf1N1B4_1145 - 1-phosphofructokinase (EC 2.7.1.56), at 1,288,532 to 1,289,473
_1145
Pf1N1B4_1146 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) , at 1,289,473 to 1,292,334
_1146
Pf1N1B4_1147 - Fructose repressor FruR, LacI family, at 1,292,636 to 1,293,631
_1147
Position (kb)
1289
1290
1291
1292
1293 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1288.513 kb on - strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1289.197 kb on + strand, within Pf1N1B4_1145 at 1289.489 kb on - strand at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.350 kb on - strand, within Pf1N1B4_1146 at 1290.350 kb on - strand at 1290.350 kb on - strand, within Pf1N1B4_1146 at 1290.568 kb on - strand, within Pf1N1B4_1146 at 1290.695 kb on + strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1292.054 kb on + strand at 1292.055 kb on - strand at 1292.163 kb on + strand at 1292.163 kb on + strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.219 kb on + strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.263 kb on + strand at 1292.263 kb on + strand at 1292.263 kb on + strand at 1292.264 kb on - strand at 1292.264 kb on - strand at 1292.264 kb on - strand at 1292.317 kb on + strand at 1292.317 kb on + strand at 1292.318 kb on - strand at 1292.318 kb on - strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.369 kb on - strand at 1292.391 kb on - strand at 1292.391 kb on - strand at 1292.391 kb on - strand at 1292.473 kb on + strand at 1292.617 kb on + strand at 1292.618 kb on - strand at 1292.630 kb on - strand at 1292.651 kb on - strand at 1292.651 kb on - strand at 1292.651 kb on - strand at 1292.690 kb on + strand at 1292.690 kb on + strand at 1292.690 kb on + strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.722 kb on + strand at 1292.722 kb on + strand at 1292.722 kb on + strand at 1292.723 kb on - strand at 1292.723 kb on - strand at 1292.723 kb on - strand at 1292.861 kb on + strand, within Pf1N1B4_1147 at 1292.862 kb on - strand, within Pf1N1B4_1147 at 1292.924 kb on - strand, within Pf1N1B4_1147 at 1292.961 kb on + strand at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1293.035 kb on + strand at 1293.035 kb on + strand, within Pf1N1B4_1147 at 1293.146 kb on - strand, within Pf1N1B4_1147 at 1293.211 kb on + strand, within Pf1N1B4_1147 at 1293.211 kb on + strand, within Pf1N1B4_1147 at 1293.212 kb on - strand, within Pf1N1B4_1147
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 1,288,513 - -0.8 1,288,533 + +0.4 1,288,533 + +2.0 1,288,533 + +0.6 1,288,533 + +1.3 1,288,534 - +0.0 1,288,534 - +0.2 1,288,534 - -0.1 1,288,534 - +1.2 1,288,534 - +0.2 1,288,534 - +1.5 1,289,197 + Pf1N1B4_1145 0.71 -1.4 1,289,489 - -0.0 1,289,804 + Pf1N1B4_1146 0.12 +0.2 1,289,804 + Pf1N1B4_1146 0.12 +1.6 1,289,804 + Pf1N1B4_1146 0.12 +0.4 1,289,804 + Pf1N1B4_1146 0.12 +0.6 1,289,805 - Pf1N1B4_1146 0.12 +1.2 1,289,805 - Pf1N1B4_1146 0.12 -0.4 1,289,805 - Pf1N1B4_1146 0.12 -0.1 1,289,805 - Pf1N1B4_1146 0.12 -1.2 1,289,805 - Pf1N1B4_1146 0.12 +1.6 1,289,805 - Pf1N1B4_1146 0.12 +3.0 1,289,805 - Pf1N1B4_1146 0.12 +0.2 1,289,805 - +0.6 1,289,805 - Pf1N1B4_1146 0.12 -0.1 1,289,805 - Pf1N1B4_1146 0.12 +1.0 1,289,805 - Pf1N1B4_1146 0.12 +0.2 1,289,805 - Pf1N1B4_1146 0.12 +0.9 1,289,805 - Pf1N1B4_1146 0.12 +0.2 1,289,805 - Pf1N1B4_1146 0.12 +0.9 1,290,349 + Pf1N1B4_1146 0.31 -0.8 1,290,349 + Pf1N1B4_1146 0.31 +0.2 1,290,350 - Pf1N1B4_1146 0.31 +0.5 1,290,350 - +0.2 1,290,350 - Pf1N1B4_1146 0.31 +0.8 1,290,568 - Pf1N1B4_1146 0.38 +0.8 1,290,695 + Pf1N1B4_1146 0.43 +0.8 1,290,696 - Pf1N1B4_1146 0.43 +1.6 1,290,696 - Pf1N1B4_1146 0.43 +1.3 1,290,696 - Pf1N1B4_1146 0.43 +1.8 1,290,696 - Pf1N1B4_1146 0.43 +0.5 1,290,696 - Pf1N1B4_1146 0.43 +0.4 1,290,696 - Pf1N1B4_1146 0.43 -0.4 1,290,696 - Pf1N1B4_1146 0.43 +0.7 1,290,696 - Pf1N1B4_1146 0.43 -0.6 1,290,696 - Pf1N1B4_1146 0.43 +1.0 1,290,696 - Pf1N1B4_1146 0.43 +2.2 1,290,696 - Pf1N1B4_1146 0.43 -0.4 1,290,696 - Pf1N1B4_1146 0.43 -2.8 1,290,696 - Pf1N1B4_1146 0.43 -0.8 1,290,696 - Pf1N1B4_1146 0.43 -0.6 1,290,696 - Pf1N1B4_1146 0.43 +0.8 1,291,621 + Pf1N1B4_1146 0.75 +0.5 1,291,621 + Pf1N1B4_1146 0.75 +0.9 1,291,621 + Pf1N1B4_1146 0.75 -0.6 1,291,621 + Pf1N1B4_1146 0.75 -1.2 1,291,622 - Pf1N1B4_1146 0.75 +1.1 1,291,622 - Pf1N1B4_1146 0.75 +1.5 1,291,622 - Pf1N1B4_1146 0.75 -0.6 1,291,622 - Pf1N1B4_1146 0.75 +0.8 1,292,054 + +0.8 1,292,055 - +0.4 1,292,163 + +0.2 1,292,163 + -2.0 1,292,164 - +1.2 1,292,164 - -0.8 1,292,164 - +0.2 1,292,164 - +3.6 1,292,164 - -1.4 1,292,219 + +1.2 1,292,220 - +0.5 1,292,220 - +3.2 1,292,220 - -0.2 1,292,220 - +1.0 1,292,220 - +0.9 1,292,220 - +2.2 1,292,220 - -0.6 1,292,263 + +0.6 1,292,263 + +1.2 1,292,263 + +1.2 1,292,264 - +0.6 1,292,264 - -1.2 1,292,264 - -1.3 1,292,317 + +0.2 1,292,317 + -0.3 1,292,318 - +0.3 1,292,318 - -0.6 1,292,368 + +0.7 1,292,368 + -0.8 1,292,368 + -0.2 1,292,368 + -1.6 1,292,369 - +0.3 1,292,391 - +0.2 1,292,391 - +0.2 1,292,391 - +0.0 1,292,473 + +0.2 1,292,617 + +0.9 1,292,618 - +0.5 1,292,630 - -0.2 1,292,651 - +0.2 1,292,651 - -0.4 1,292,651 - +0.6 1,292,690 + -0.2 1,292,690 + -0.8 1,292,690 + -0.6 1,292,691 - +0.5 1,292,691 - +1.2 1,292,691 - -2.2 1,292,691 - -0.8 1,292,691 - -0.1 1,292,691 - +0.2 1,292,691 - +2.5 1,292,691 - +0.6 1,292,691 - +1.3 1,292,691 - +0.0 1,292,691 - -0.4 1,292,691 - +1.8 1,292,691 - -0.0 1,292,722 + -0.2 1,292,722 + -3.3 1,292,722 + +1.8 1,292,723 - +0.7 1,292,723 - +0.0 1,292,723 - +0.2 1,292,861 + Pf1N1B4_1147 0.23 -0.7 1,292,862 - Pf1N1B4_1147 0.23 +1.2 1,292,924 - Pf1N1B4_1147 0.29 -0.2 1,292,961 + -0.6 1,292,961 + Pf1N1B4_1147 0.33 -1.1 1,292,961 + Pf1N1B4_1147 0.33 +0.2 1,292,961 + Pf1N1B4_1147 0.33 -1.2 1,292,962 - Pf1N1B4_1147 0.33 +0.3 1,292,962 - Pf1N1B4_1147 0.33 +0.9 1,292,962 - Pf1N1B4_1147 0.33 -3.4 1,292,962 - Pf1N1B4_1147 0.33 -0.8 1,292,962 - Pf1N1B4_1147 0.33 +0.2 1,292,962 - Pf1N1B4_1147 0.33 -1.4 1,293,035 + -1.4 1,293,035 + Pf1N1B4_1147 0.40 -0.2 1,293,146 - Pf1N1B4_1147 0.51 +0.7 1,293,211 + Pf1N1B4_1147 0.58 +1.5 1,293,211 + Pf1N1B4_1147 0.58 -0.4 1,293,212 - Pf1N1B4_1147 0.58 -1.4
Or see this region's nucleotide sequence