Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3753 and Shew_3754 are separated by 93 nucleotides Shew_3754 and Shew_3755 are separated by 65 nucleotides Shew_3755 and Shew_3756 are separated by 150 nucleotides
Shew_3753: Shew_3753 - hypothetical protein (RefSeq), at 4,469,926 to 4,470,450
_3753
Shew_3754: Shew_3754 - hypothetical protein (RefSeq), at 4,470,544 to 4,471,221
_3754
Shew_3755: Shew_3755 - thioesterase superfamily protein (RefSeq), at 4,471,287 to 4,471,766
_3755
Shew_3756: Shew_3756 - histidine ammonia-lyase (RefSeq), at 4,471,917 to 4,473,449
_3756
Position (kb)
4471
4472 Strain fitness (log2 ratio)
-2
-1
0
1 at 4470.714 kb on + strand, within Shew_3754 at 4470.736 kb on - strand, within Shew_3754 at 4470.818 kb on - strand, within Shew_3754 at 4470.874 kb on + strand, within Shew_3754 at 4470.987 kb on - strand, within Shew_3754 at 4471.050 kb on + strand, within Shew_3754 at 4471.058 kb on - strand, within Shew_3754 at 4471.159 kb on + strand at 4471.167 kb on - strand at 4471.378 kb on - strand, within Shew_3755 at 4471.473 kb on - strand, within Shew_3755 at 4471.830 kb on + strand at 4471.921 kb on + strand at 4471.921 kb on + strand at 4472.050 kb on + strand at 4472.058 kb on - strand at 4472.058 kb on - strand at 4472.058 kb on - strand at 4472.087 kb on - strand, within Shew_3756 at 4472.241 kb on + strand, within Shew_3756 at 4472.284 kb on - strand, within Shew_3756 at 4472.400 kb on + strand, within Shew_3756 at 4472.647 kb on - strand, within Shew_3756 at 4472.744 kb on + strand, within Shew_3756 at 4472.744 kb on + strand, within Shew_3756
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 4,470,714 + Shew_3754 0.25 -0.5 4,470,736 - Shew_3754 0.28 +0.5 4,470,818 - Shew_3754 0.40 +0.2 4,470,874 + Shew_3754 0.49 -0.3 4,470,987 - Shew_3754 0.65 +0.4 4,471,050 + Shew_3754 0.75 +0.3 4,471,058 - Shew_3754 0.76 +0.3 4,471,159 + +0.2 4,471,167 - -0.4 4,471,378 - Shew_3755 0.19 -2.0 4,471,473 - Shew_3755 0.39 -0.1 4,471,830 + +0.6 4,471,921 + -0.1 4,471,921 + +0.8 4,472,050 + -0.4 4,472,058 - -0.1 4,472,058 - -0.1 4,472,058 - +0.5 4,472,087 - Shew_3756 0.11 -0.1 4,472,241 + Shew_3756 0.21 -1.4 4,472,284 - Shew_3756 0.24 +0.8 4,472,400 + Shew_3756 0.32 +0.4 4,472,647 - Shew_3756 0.48 +0.1 4,472,744 + Shew_3756 0.54 -0.9 4,472,744 + Shew_3756 0.54 +0.9
Or see this region's nucleotide sequence