Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1858 and Shew_1859 are separated by 254 nucleotides Shew_1859 and Shew_1860 are separated by 139 nucleotides Shew_1860 and Shew_1861 are separated by 6 nucleotides
Shew_1858: Shew_1858 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,137,104 to 2,138,291
_1858
Shew_1859: Shew_1859 - carbonate dehydratase (RefSeq), at 2,138,546 to 2,139,157
_1859
Shew_1860: Shew_1860 - alpha/beta hydrolase fold (RefSeq), at 2,139,297 to 2,140,130
_1860
Shew_1861: Shew_1861 - peptidase M15B and M15C, D,D-carboxypeptidase VanY/endolysin (RefSeq), at 2,140,137 to 2,140,889
_1861
Position (kb)
2138
2139
2140 Strain fitness (log2 ratio)
-1
0
1 at 2138.930 kb on + strand, within Shew_1859 at 2139.227 kb on + strand at 2139.448 kb on + strand, within Shew_1860 at 2139.450 kb on - strand, within Shew_1860 at 2139.712 kb on + strand, within Shew_1860 at 2139.886 kb on - strand, within Shew_1860 at 2139.902 kb on - strand, within Shew_1860 at 2140.028 kb on - strand, within Shew_1860
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 2,138,930 + Shew_1859 0.63 +0.8 2,139,227 + -1.4 2,139,448 + Shew_1860 0.18 +0.1 2,139,450 - Shew_1860 0.18 -0.2 2,139,712 + Shew_1860 0.50 -0.1 2,139,886 - Shew_1860 0.71 +0.3 2,139,902 - Shew_1860 0.73 +0.9 2,140,028 - Shew_1860 0.88 +0.0
Or see this region's nucleotide sequence