Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1518 and Shew_1519 are separated by 151 nucleotides Shew_1519 and Shew_1520 are separated by 99 nucleotides Shew_1520 and Shew_1521 are separated by 108 nucleotides
Shew_1518: Shew_1518 - hypothetical protein (RefSeq), at 1,740,943 to 1,741,689
_1518
Shew_1519: Shew_1519 - hypothetical protein (RefSeq), at 1,741,841 to 1,742,857
_1519
Shew_1520: Shew_1520 - ribonuclease T (RefSeq), at 1,742,957 to 1,743,730
_1520
Shew_1521: Shew_1521 - OmpA/MotB domain-containing protein (RefSeq), at 1,743,839 to 1,744,699
_1521
Position (kb)
1741
1742
1743 Strain fitness (log2 ratio)
-1
0
1 at 1741.011 kb on + strand at 1741.595 kb on - strand, within Shew_1518 at 1741.613 kb on + strand, within Shew_1518 at 1742.196 kb on - strand, within Shew_1519 at 1742.511 kb on - strand, within Shew_1519 at 1742.543 kb on - strand, within Shew_1519 at 1742.757 kb on - strand at 1742.780 kb on + strand at 1742.841 kb on + strand at 1742.844 kb on + strand at 1742.844 kb on + strand at 1742.844 kb on + strand at 1742.971 kb on - strand at 1743.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 1,741,011 + +0.7 1,741,595 - Shew_1518 0.87 +0.6 1,741,613 + Shew_1518 0.90 +1.0 1,742,196 - Shew_1519 0.35 +1.0 1,742,511 - Shew_1519 0.66 +0.4 1,742,543 - Shew_1519 0.69 +0.3 1,742,757 - -0.6 1,742,780 + +0.3 1,742,841 + -1.6 1,742,844 + -0.0 1,742,844 + +0.1 1,742,844 + +0.8 1,742,971 - +0.5 1,743,692 - +0.8
Or see this region's nucleotide sequence