Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0671 and Shew_0672 are separated by 232 nucleotides Shew_0672 and Shew_0673 are separated by 8 nucleotides Shew_0673 and Shew_0674 are separated by 98 nucleotides
Shew_0671: Shew_0671 - phospholipase A(1) (RefSeq), at 764,130 to 765,056
_0671
Shew_0672: Shew_0672 - uroporphyrin-III C-methyltransferase (RefSeq), at 765,289 to 766,110
_0672
Shew_0673: Shew_0673 - hypothetical protein (RefSeq), at 766,119 to 766,955
_0673
Shew_0674: Shew_0674 - major facilitator transporter (RefSeq), at 767,054 to 768,220
_0674
Position (kb) 765
766
767 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 764.470 kb on - strand, within Shew_0671 at 764.536 kb on + strand, within Shew_0671 at 764.667 kb on + strand, within Shew_0671 at 764.758 kb on - strand, within Shew_0671 at 764.908 kb on - strand, within Shew_0671 at 764.952 kb on - strand, within Shew_0671 at 765.464 kb on + strand, within Shew_0672 at 765.504 kb on - strand, within Shew_0672 at 765.581 kb on - strand, within Shew_0672 at 765.960 kb on + strand, within Shew_0672 at 766.160 kb on - strand at 766.292 kb on + strand, within Shew_0673 at 766.365 kb on + strand, within Shew_0673 at 766.526 kb on - strand, within Shew_0673 at 766.618 kb on + strand, within Shew_0673 at 766.724 kb on - strand, within Shew_0673 at 766.935 kb on - strand at 767.040 kb on - strand at 767.046 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 764,470 - Shew_0671 0.37 -0.6 764,536 + Shew_0671 0.44 +0.6 764,667 + Shew_0671 0.58 -0.3 764,758 - Shew_0671 0.68 +0.7 764,908 - Shew_0671 0.84 +0.0 764,952 - Shew_0671 0.89 +0.0 765,464 + Shew_0672 0.21 -0.1 765,504 - Shew_0672 0.26 -2.8 765,581 - Shew_0672 0.36 -1.3 765,960 + Shew_0672 0.82 -0.5 766,160 - -0.6 766,292 + Shew_0673 0.21 -0.2 766,365 + Shew_0673 0.29 -0.6 766,526 - Shew_0673 0.49 +0.5 766,618 + Shew_0673 0.60 +0.3 766,724 - Shew_0673 0.72 -0.4 766,935 - +0.3 767,040 - -0.1 767,046 + +0.5
Or see this region's nucleotide sequence