Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0140 and Shew_R0001 are separated by 631 nucleotides Shew_R0001 and Shew_R0002 are separated by 57 nucleotides Shew_R0002 and Shew_R0003 are separated by 52 nucleotides Shew_R0003 and Shew_R0004 are separated by 245 nucleotides
Shew_0140: Shew_0140 - RNP-1-like RNA-binding protein (RefSeq), at 149,997 to 150,455
_0140
Shew_R0001: Shew_R0001 - 16S ribosomal RNA (RefSeq), at 151,087 to 152,641
_R0001
Shew_R0002: Shew_R0002 - tRNA-Ile (RefSeq), at 152,699 to 152,775
_R0002
Shew_R0003: Shew_R0003 - tRNA-Ala (RefSeq), at 152,828 to 152,903
_R0003
Shew_R0004: Shew_R0004 - 23S ribosomal RNA (RefSeq), at 153,149 to 156,041
_R0004
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 150.135 kb on + strand, within Shew_0140 at 150.201 kb on - strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.246 kb on - strand, within Shew_0140 at 150.278 kb on + strand, within Shew_0140 at 150.280 kb on + strand, within Shew_0140 at 150.288 kb on - strand, within Shew_0140 at 150.318 kb on - strand, within Shew_0140 at 150.385 kb on + strand, within Shew_0140 at 150.393 kb on - strand, within Shew_0140 at 150.393 kb on - strand, within Shew_0140
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 150,135 + Shew_0140 0.30 -0.2 150,201 - Shew_0140 0.44 -1.0 150,238 + Shew_0140 0.53 +1.3 150,238 + Shew_0140 0.53 -0.3 150,238 + Shew_0140 0.53 +0.0 150,238 + Shew_0140 0.53 -0.2 150,238 + Shew_0140 0.53 +1.1 150,238 + Shew_0140 0.53 -0.7 150,246 - Shew_0140 0.54 -0.3 150,278 + Shew_0140 0.61 -0.2 150,280 + Shew_0140 0.62 -0.3 150,288 - Shew_0140 0.63 -0.2 150,318 - Shew_0140 0.70 +0.1 150,385 + Shew_0140 0.85 -2.7 150,393 - Shew_0140 0.86 -0.6 150,393 - Shew_0140 0.86 -0.2
Or see this region's nucleotide sequence