Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3610 and Rru_A3611 are separated by 154 nucleotides Rru_A3611 and Rru_A3612 overlap by 8 nucleotides Rru_A3612 and Rru_A3613 are separated by 17 nucleotides Rru_A3613 and Rru_A3614 overlap by 4 nucleotides
Rru_A3610: Rru_A3610 - protein export chaperone SecB (NCBI), at 4,149,880 to 4,150,362
_A3610
Rru_A3611: Rru_A3611 - DNA polymerase III, epsilon subunit (NCBI), at 4,150,517 to 4,151,209
_A3611
Rru_A3612: Rru_A3612 - Dephospho-CoA kinase (NCBI), at 4,151,202 to 4,151,891
_A3612
Rru_A3613: Rru_A3613 - Quinate/Shikimate 5-dehydrogenase (NCBI), at 4,151,909 to 4,152,760
_A3613
Rru_A3614: Rru_A3614 - Maf-like protein (NCBI), at 4,152,757 to 4,153,365
_A3614
Position (kb)
4151
4152 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4150.300 kb on + strand, within Rru_A3610 at 4150.300 kb on + strand, within Rru_A3610 at 4150.301 kb on - strand, within Rru_A3610 at 4150.301 kb on - strand, within Rru_A3610 at 4150.518 kb on + strand at 4150.518 kb on + strand at 4150.519 kb on - strand at 4150.519 kb on - strand at 4151.219 kb on - strand at 4151.219 kb on - strand at 4151.910 kb on + strand at 4151.910 kb on + strand at 4152.811 kb on + strand at 4152.881 kb on + strand, within Rru_A3614 at 4152.881 kb on + strand, within Rru_A3614 at 4152.882 kb on - strand, within Rru_A3614 at 4152.882 kb on - strand, within Rru_A3614
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 4,150,300 + Rru_A3610 0.87 -2.4 4,150,300 + Rru_A3610 0.87 +1.8 4,150,301 - Rru_A3610 0.87 +0.8 4,150,301 - Rru_A3610 0.87 +0.4 4,150,518 + -1.1 4,150,518 + +1.1 4,150,519 - -1.8 4,150,519 - -3.8 4,151,219 - -3.8 4,151,219 - +0.6 4,151,910 + +0.0 4,151,910 + +2.1 4,152,811 + -0.8 4,152,881 + Rru_A3614 0.20 -1.8 4,152,881 + Rru_A3614 0.20 -2.5 4,152,882 - Rru_A3614 0.21 -2.9 4,152,882 - Rru_A3614 0.21 +1.1
Or see this region's nucleotide sequence