Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3520 and Rru_A3521 are separated by 73 nucleotides Rru_A3521 and Rru_A3522 are separated by 54 nucleotides Rru_A3522 and Rru_A3523 are separated by 164 nucleotides
Rru_A3520: Rru_A3520 - Protein of unknown function UPF0118 (NCBI), at 4,052,063 to 4,053,367
_A3520
Rru_A3521: Rru_A3521 - Ribosomal protein L35 (NCBI), at 4,053,441 to 4,053,638
_A3521
Rru_A3522: Rru_A3522 - Ribosomal protein L20 (NCBI), at 4,053,693 to 4,054,070
_A3522
Rru_A3523: Rru_A3523 - Phenylalanyl-tRNA synthetase, alpha subunit (NCBI), at 4,054,235 to 4,055,311
_A3523
Position (kb)
4053
4054
4055 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4052.707 kb on + strand, within Rru_A3520 at 4052.758 kb on + strand, within Rru_A3520 at 4052.776 kb on + strand, within Rru_A3520 at 4052.776 kb on + strand, within Rru_A3520 at 4052.777 kb on - strand, within Rru_A3520 at 4052.777 kb on - strand, within Rru_A3520 at 4052.777 kb on - strand, within Rru_A3520 at 4053.075 kb on + strand, within Rru_A3520 at 4053.075 kb on + strand, within Rru_A3520 at 4053.305 kb on + strand at 4053.305 kb on + strand at 4053.305 kb on + strand at 4053.305 kb on + strand at 4053.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 4,052,707 + Rru_A3520 0.49 -0.0 4,052,758 + Rru_A3520 0.53 -2.3 4,052,776 + Rru_A3520 0.55 -0.3 4,052,776 + Rru_A3520 0.55 +2.3 4,052,777 - Rru_A3520 0.55 -2.7 4,052,777 - Rru_A3520 0.55 -2.6 4,052,777 - Rru_A3520 0.55 +1.9 4,053,075 + Rru_A3520 0.78 +0.9 4,053,075 + Rru_A3520 0.78 -0.7 4,053,305 + +0.5 4,053,305 + -3.4 4,053,305 + +0.4 4,053,305 + +1.1 4,053,305 + -1.8
Or see this region's nucleotide sequence