Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3316 and Rru_AR0065 are separated by 76 nucleotides Rru_AR0065 and Rru_A3317 are separated by 387 nucleotides Rru_A3317 and Rru_A3318 are separated by 93 nucleotides
Rru_A3316: Rru_A3316 - Phage integrase (NCBI), at 3,824,154 to 3,825,854
_A3316
Rru_AR0065: Rru_AR0065 - tRNA-Arg (NCBI), at 3,825,931 to 3,826,007
_AR0065
Rru_A3317: Rru_A3317 - hypothetical protein (NCBI), at 3,826,395 to 3,827,531
_A3317
Rru_A3318: Rru_A3318 - Gamma-glutamyltransferase (NCBI), at 3,827,625 to 3,829,229
_A3318
Position (kb)
3826
3827
3828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3825.493 kb on - strand, within Rru_A3316 at 3825.573 kb on - strand, within Rru_A3316 at 3825.677 kb on + strand, within Rru_A3316 at 3825.677 kb on + strand, within Rru_A3316 at 3825.677 kb on + strand, within Rru_A3316 at 3825.688 kb on - strand at 3825.724 kb on + strand at 3825.725 kb on - strand at 3825.748 kb on + strand at 3825.748 kb on + strand at 3825.749 kb on - strand at 3825.749 kb on - strand at 3825.791 kb on - strand at 3825.848 kb on - strand at 3825.861 kb on - strand at 3826.214 kb on + strand at 3826.216 kb on + strand at 3826.297 kb on + strand at 3826.346 kb on + strand at 3827.857 kb on - strand, within Rru_A3318 at 3828.200 kb on + strand, within Rru_A3318 at 3828.289 kb on + strand, within Rru_A3318 at 3828.289 kb on + strand, within Rru_A3318 at 3828.289 kb on + strand, within Rru_A3318 at 3828.290 kb on - strand, within Rru_A3318 at 3828.290 kb on - strand, within Rru_A3318 at 3828.290 kb on - strand, within Rru_A3318 at 3828.290 kb on - strand, within Rru_A3318 at 3828.290 kb on - strand, within Rru_A3318 at 3828.477 kb on - strand, within Rru_A3318
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,825,493 - Rru_A3316 0.79 +2.0 3,825,573 - Rru_A3316 0.83 +0.9 3,825,677 + Rru_A3316 0.90 -1.3 3,825,677 + Rru_A3316 0.90 +1.2 3,825,677 + Rru_A3316 0.90 -0.8 3,825,688 - +2.4 3,825,724 + +1.8 3,825,725 - +0.4 3,825,748 + +2.8 3,825,748 + +0.9 3,825,749 - +2.2 3,825,749 - -0.2 3,825,791 - -1.1 3,825,848 - -3.6 3,825,861 - -2.6 3,826,214 + +0.9 3,826,216 + +0.3 3,826,297 + +2.4 3,826,346 + -1.4 3,827,857 - Rru_A3318 0.14 -1.8 3,828,200 + Rru_A3318 0.36 +0.0 3,828,289 + Rru_A3318 0.41 -1.6 3,828,289 + Rru_A3318 0.41 +0.4 3,828,289 + Rru_A3318 0.41 -0.8 3,828,290 - Rru_A3318 0.41 -2.6 3,828,290 - Rru_A3318 0.41 -3.1 3,828,290 - Rru_A3318 0.41 +2.0 3,828,290 - Rru_A3318 0.41 -0.6 3,828,290 - Rru_A3318 0.41 -1.9 3,828,477 - Rru_A3318 0.53 -1.7
Or see this region's nucleotide sequence