Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3278 and Rru_A3279 are separated by 264 nucleotides Rru_A3279 and Rru_A3280 are separated by 258 nucleotides Rru_A3280 and Rru_A3281 are separated by 1 nucleotides
Rru_A3278: Rru_A3278 - ADP-L-glycero-D-manno-heptose-6-epimerase (NCBI), at 3,776,040 to 3,777,023
_A3278
Rru_A3279: Rru_A3279 - Protein of unknown function DUF526 (NCBI), at 3,777,288 to 3,777,656
_A3279
Rru_A3280: Rru_A3280 - hypothetical protein (NCBI), at 3,777,915 to 3,778,415
_A3280
Rru_A3281: Rru_A3281 - Delta 1-pyrroline-5-carboxylate reductase (NCBI), at 3,778,417 to 3,779,241
_A3281
Position (kb)
3777
3778 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3777.082 kb on + strand at 3777.083 kb on - strand at 3777.083 kb on - strand at 3777.083 kb on - strand at 3777.083 kb on - strand at 3777.083 kb on - strand at 3777.084 kb on + strand at 3777.084 kb on + strand at 3777.084 kb on + strand at 3777.085 kb on - strand at 3777.085 kb on - strand at 3777.085 kb on - strand at 3777.085 kb on - strand at 3777.085 kb on - strand at 3777.202 kb on + strand at 3777.202 kb on + strand at 3777.262 kb on + strand at 3777.263 kb on - strand at 3777.654 kb on + strand at 3777.654 kb on + strand at 3778.059 kb on + strand, within Rru_A3280 at 3778.326 kb on + strand, within Rru_A3280 at 3778.326 kb on + strand, within Rru_A3280
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,777,082 + +1.3 3,777,083 - +0.1 3,777,083 - -2.9 3,777,083 - +1.3 3,777,083 - +1.3 3,777,083 - -1.1 3,777,084 + +0.2 3,777,084 + -0.2 3,777,084 + +1.7 3,777,085 - -1.0 3,777,085 - +0.4 3,777,085 - +0.0 3,777,085 - -0.4 3,777,085 - +0.0 3,777,202 + +0.9 3,777,202 + +1.2 3,777,262 + -1.7 3,777,263 - -0.3 3,777,654 + -1.7 3,777,654 + +0.0 3,778,059 + Rru_A3280 0.29 +0.4 3,778,326 + Rru_A3280 0.82 -0.3 3,778,326 + Rru_A3280 0.82 -1.1
Or see this region's nucleotide sequence