Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3248 and Rru_A3249 are separated by 11 nucleotides Rru_A3249 and Rru_A3250 are separated by 57 nucleotides Rru_A3250 and Rru_A3251 are separated by 101 nucleotides
Rru_A3248: Rru_A3248 - Chromosome segregation protein SMC (NCBI), at 3,741,669 to 3,745,172
_A3248
Rru_A3249: Rru_A3249 - DSBA oxidoreductase (NCBI), at 3,745,184 to 3,745,837
_A3249
Rru_A3250: Rru_A3250 - Protein of unknown function DUF1159 (NCBI), at 3,745,895 to 3,746,467
_A3250
Rru_A3251: Rru_A3251 - A/G-specific adenine glycosylase MutY (NCBI), at 3,746,569 to 3,747,648
_A3251
Position (kb)
3745
3746
3747 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3745.838 kb on + strand at 3745.839 kb on - strand at 3745.839 kb on - strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.841 kb on - strand at 3745.841 kb on - strand at 3745.841 kb on - strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.843 kb on - strand at 3745.843 kb on - strand at 3745.843 kb on - strand at 3746.524 kb on + strand at 3746.524 kb on + strand at 3746.525 kb on - strand at 3746.525 kb on - strand at 3746.525 kb on - strand at 3746.635 kb on + strand at 3746.755 kb on + strand, within Rru_A3251 at 3746.755 kb on + strand, within Rru_A3251 at 3746.755 kb on + strand, within Rru_A3251 at 3746.756 kb on - strand, within Rru_A3251 at 3746.852 kb on - strand, within Rru_A3251 at 3746.962 kb on + strand, within Rru_A3251 at 3746.962 kb on + strand, within Rru_A3251 at 3746.962 kb on + strand, within Rru_A3251 at 3746.963 kb on - strand, within Rru_A3251 at 3746.963 kb on - strand, within Rru_A3251 at 3746.963 kb on - strand, within Rru_A3251 at 3746.963 kb on - strand, within Rru_A3251 at 3746.963 kb on - strand, within Rru_A3251 at 3746.964 kb on + strand, within Rru_A3251 at 3747.024 kb on + strand, within Rru_A3251
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,745,838 + -0.7 3,745,839 - -1.2 3,745,839 - +1.4 3,745,840 + +1.9 3,745,840 + +0.8 3,745,840 + +0.1 3,745,840 + -0.7 3,745,840 + +0.4 3,745,841 - +0.6 3,745,841 - -1.0 3,745,841 - +1.4 3,745,842 + -0.8 3,745,842 + +1.1 3,745,842 + -3.0 3,745,842 + +0.6 3,745,842 + -1.0 3,745,843 - -2.1 3,745,843 - -4.1 3,745,843 - -1.6 3,746,524 + +0.8 3,746,524 + +0.4 3,746,525 - -1.0 3,746,525 - -1.8 3,746,525 - -1.6 3,746,635 + -0.9 3,746,755 + Rru_A3251 0.17 -0.1 3,746,755 + Rru_A3251 0.17 +0.5 3,746,755 + Rru_A3251 0.17 -3.1 3,746,756 - Rru_A3251 0.17 +2.0 3,746,852 - Rru_A3251 0.26 -0.6 3,746,962 + Rru_A3251 0.36 -0.8 3,746,962 + Rru_A3251 0.36 -2.1 3,746,962 + Rru_A3251 0.36 -1.6 3,746,963 - Rru_A3251 0.36 +2.7 3,746,963 - Rru_A3251 0.36 -1.9 3,746,963 - Rru_A3251 0.36 -1.0 3,746,963 - Rru_A3251 0.36 +1.0 3,746,963 - Rru_A3251 0.36 -1.9 3,746,964 + Rru_A3251 0.37 -2.8 3,747,024 + Rru_A3251 0.42 -3.0
Or see this region's nucleotide sequence