Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3246 and Rru_A3247 overlap by 8 nucleotides Rru_A3247 and Rru_A3248 are separated by 198 nucleotides Rru_A3248 and Rru_A3249 are separated by 11 nucleotides Rru_A3249 and Rru_A3250 are separated by 57 nucleotides
Rru_A3246: Rru_A3246 - H+-transporting two-sector ATPase, A subunit (NCBI), at 3,740,381 to 3,741,106
_A3246
Rru_A3247: Rru_A3247 - hypothetical protein (NCBI), at 3,741,099 to 3,741,470
_A3247
Rru_A3248: Rru_A3248 - Chromosome segregation protein SMC (NCBI), at 3,741,669 to 3,745,172
_A3248
Rru_A3249: Rru_A3249 - DSBA oxidoreductase (NCBI), at 3,745,184 to 3,745,837
_A3249
Rru_A3250: Rru_A3250 - Protein of unknown function DUF1159 (NCBI), at 3,745,895 to 3,746,467
_A3250
Position (kb)
3741
3742
3743
3744
3745
3746 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3741.191 kb on - strand, within Rru_A3247 at 3741.671 kb on - strand at 3745.838 kb on + strand at 3745.839 kb on - strand at 3745.839 kb on - strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.840 kb on + strand at 3745.841 kb on - strand at 3745.841 kb on - strand at 3745.841 kb on - strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.842 kb on + strand at 3745.843 kb on - strand at 3745.843 kb on - strand at 3745.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,741,191 - Rru_A3247 0.25 -1.9 3,741,671 - -2.2 3,745,838 + -0.7 3,745,839 - -1.2 3,745,839 - +1.4 3,745,840 + +1.9 3,745,840 + +0.8 3,745,840 + +0.1 3,745,840 + -0.7 3,745,840 + +0.4 3,745,841 - +0.6 3,745,841 - -1.0 3,745,841 - +1.4 3,745,842 + -0.8 3,745,842 + +1.1 3,745,842 + -3.0 3,745,842 + +0.6 3,745,842 + -1.0 3,745,843 - -2.1 3,745,843 - -4.1 3,745,843 - -1.6
Or see this region's nucleotide sequence