Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3176 and Rru_A3177 are separated by 8 nucleotides Rru_A3177 and Rru_A3178 are separated by 168 nucleotides Rru_A3178 and Rru_A3179 overlap by 4 nucleotides
Rru_A3176: Rru_A3176 - Glutamyl-tRNA(Gln) amidotransferase A subunit (NCBI), at 3,668,276 to 3,669,778
_A3176
Rru_A3177: Rru_A3177 - Glutamyl-tRNA(Gln) amidotransferase C subunit (NCBI), at 3,669,787 to 3,670,074
_A3177
Rru_A3178: Rru_A3178 - Holliday junction resolvase YqgF (NCBI), at 3,670,243 to 3,670,728
_A3178
Rru_A3179: Rru_A3179 - GCN5-related N-acetyltransferase (NCBI), at 3,670,725 to 3,671,351
_A3179
Position (kb)
3669
3670
3671 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3670.110 kb on - strand at 3670.727 kb on - strand at 3670.847 kb on - strand, within Rru_A3179 at 3670.847 kb on - strand, within Rru_A3179 at 3670.847 kb on - strand, within Rru_A3179 at 3670.886 kb on + strand, within Rru_A3179 at 3670.886 kb on + strand, within Rru_A3179 at 3670.887 kb on - strand, within Rru_A3179 at 3671.068 kb on + strand, within Rru_A3179 at 3671.069 kb on - strand, within Rru_A3179 at 3671.069 kb on - strand, within Rru_A3179 at 3671.069 kb on - strand, within Rru_A3179
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,670,110 - -2.4 3,670,727 - -1.9 3,670,847 - Rru_A3179 0.19 -1.7 3,670,847 - Rru_A3179 0.19 -3.3 3,670,847 - Rru_A3179 0.19 +0.8 3,670,886 + Rru_A3179 0.26 +0.0 3,670,886 + Rru_A3179 0.26 -0.2 3,670,887 - Rru_A3179 0.26 +0.2 3,671,068 + Rru_A3179 0.55 -2.4 3,671,069 - Rru_A3179 0.55 -2.5 3,671,069 - Rru_A3179 0.55 -0.6 3,671,069 - Rru_A3179 0.55 -0.4
Or see this region's nucleotide sequence