Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3121 and Rru_A3122 overlap by 4 nucleotides Rru_A3122 and Rru_A3123 are separated by 2 nucleotides Rru_A3123 and Rru_A3124 overlap by 4 nucleotides
Rru_A3121: Rru_A3121 - Asparagine synthase, glutamine-hydrolyzing (NCBI), at 3,600,181 to 3,602,106
_A3121
Rru_A3122: Rru_A3122 - hypothetical protein (NCBI), at 3,602,103 to 3,602,387
_A3122
Rru_A3123: Rru_A3123 - esterase/lipase/thioesterase family active site (NCBI), at 3,602,390 to 3,603,160
_A3123
Rru_A3124: Rru_A3124 - Alpha/beta hydrolase fold (NCBI), at 3,603,157 to 3,603,999
_A3124
Position (kb)
3602
3603 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3601.114 kb on + strand, within Rru_A3121 at 3601.114 kb on + strand, within Rru_A3121 at 3601.138 kb on - strand, within Rru_A3121 at 3601.324 kb on - strand, within Rru_A3121 at 3601.481 kb on - strand, within Rru_A3121 at 3601.481 kb on - strand, within Rru_A3121 at 3601.528 kb on + strand, within Rru_A3121 at 3601.528 kb on + strand, within Rru_A3121 at 3601.529 kb on - strand, within Rru_A3121 at 3601.529 kb on - strand, within Rru_A3121 at 3601.529 kb on - strand, within Rru_A3121 at 3601.590 kb on + strand, within Rru_A3121 at 3601.590 kb on + strand, within Rru_A3121 at 3601.591 kb on - strand, within Rru_A3121 at 3601.591 kb on - strand, within Rru_A3121 at 3601.591 kb on - strand, within Rru_A3121 at 3601.633 kb on + strand, within Rru_A3121 at 3601.633 kb on + strand, within Rru_A3121 at 3601.633 kb on + strand, within Rru_A3121 at 3601.634 kb on - strand, within Rru_A3121 at 3601.634 kb on - strand, within Rru_A3121 at 3601.634 kb on - strand, within Rru_A3121 at 3601.798 kb on + strand, within Rru_A3121 at 3601.798 kb on + strand, within Rru_A3121 at 3601.799 kb on - strand, within Rru_A3121 at 3601.799 kb on - strand, within Rru_A3121 at 3601.799 kb on - strand, within Rru_A3121 at 3601.799 kb on - strand, within Rru_A3121 at 3601.799 kb on - strand, within Rru_A3121 at 3602.029 kb on + strand at 3602.029 kb on + strand at 3602.480 kb on + strand, within Rru_A3123 at 3602.480 kb on + strand, within Rru_A3123 at 3602.481 kb on - strand, within Rru_A3123 at 3602.533 kb on - strand, within Rru_A3123 at 3602.828 kb on - strand, within Rru_A3123 at 3602.828 kb on - strand, within Rru_A3123 at 3602.828 kb on - strand, within Rru_A3123 at 3603.153 kb on + strand at 3603.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,601,114 + Rru_A3121 0.48 +1.0 3,601,114 + Rru_A3121 0.48 -0.0 3,601,138 - Rru_A3121 0.50 -0.2 3,601,324 - Rru_A3121 0.59 +0.9 3,601,481 - Rru_A3121 0.67 +1.1 3,601,481 - Rru_A3121 0.67 -0.0 3,601,528 + Rru_A3121 0.70 -1.6 3,601,528 + Rru_A3121 0.70 +0.6 3,601,529 - Rru_A3121 0.70 +1.6 3,601,529 - Rru_A3121 0.70 -1.7 3,601,529 - Rru_A3121 0.70 -1.0 3,601,590 + Rru_A3121 0.73 -1.0 3,601,590 + Rru_A3121 0.73 -2.4 3,601,591 - Rru_A3121 0.73 +1.6 3,601,591 - Rru_A3121 0.73 +2.0 3,601,591 - Rru_A3121 0.73 -0.5 3,601,633 + Rru_A3121 0.75 -0.0 3,601,633 + Rru_A3121 0.75 -1.0 3,601,633 + Rru_A3121 0.75 -3.9 3,601,634 - Rru_A3121 0.75 +1.6 3,601,634 - Rru_A3121 0.75 +1.5 3,601,634 - Rru_A3121 0.75 -0.8 3,601,798 + Rru_A3121 0.84 +0.9 3,601,798 + Rru_A3121 0.84 -0.1 3,601,799 - Rru_A3121 0.84 +0.5 3,601,799 - Rru_A3121 0.84 -1.0 3,601,799 - Rru_A3121 0.84 -1.3 3,601,799 - Rru_A3121 0.84 +3.6 3,601,799 - Rru_A3121 0.84 -1.0 3,602,029 + -3.6 3,602,029 + -0.6 3,602,480 + Rru_A3123 0.12 -2.3 3,602,480 + Rru_A3123 0.12 -1.9 3,602,481 - Rru_A3123 0.12 +2.1 3,602,533 - Rru_A3123 0.19 -1.8 3,602,828 - Rru_A3123 0.57 -0.2 3,602,828 - Rru_A3123 0.57 -0.3 3,602,828 - Rru_A3123 0.57 -0.3 3,603,153 + -0.1 3,603,153 + +0.3
Or see this region's nucleotide sequence