Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2963 and Rru_A2964 are separated by 362 nucleotides Rru_A2964 and Rru_A2965 are separated by 133 nucleotides Rru_A2965 and Rru_A2966 are separated by 165 nucleotides
Rru_A2963: Rru_A2963 - hypothetical protein (NCBI), at 3,413,261 to 3,413,746
_A2963
Rru_A2964: Rru_A2964 - MaoC-like dehydratase (NCBI), at 3,414,109 to 3,414,543
_A2964
Rru_A2965: Rru_A2965 - Riboflavin kinase / FAD synthetase (NCBI), at 3,414,677 to 3,415,630
_A2965
Rru_A2966: Rru_A2966 - Isoleucyl-tRNA synthetase, class Ia (NCBI), at 3,415,796 to 3,418,618
_A2966
Position (kb)
3414
3415
3416 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3413.781 kb on - strand at 3413.879 kb on + strand at 3413.880 kb on - strand at 3413.880 kb on - strand at 3413.880 kb on - strand at 3413.905 kb on + strand at 3413.906 kb on - strand at 3413.906 kb on - strand at 3413.906 kb on - strand at 3413.906 kb on - strand at 3413.906 kb on - strand at 3414.067 kb on + strand at 3414.067 kb on + strand at 3414.067 kb on + strand at 3414.068 kb on - strand at 3414.136 kb on + strand at 3414.202 kb on + strand, within Rru_A2964 at 3414.202 kb on + strand, within Rru_A2964 at 3414.202 kb on + strand, within Rru_A2964 at 3414.202 kb on + strand, within Rru_A2964 at 3414.203 kb on - strand, within Rru_A2964 at 3414.203 kb on - strand, within Rru_A2964 at 3414.203 kb on - strand, within Rru_A2964 at 3414.203 kb on - strand, within Rru_A2964 at 3414.256 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.361 kb on + strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.362 kb on - strand, within Rru_A2964 at 3414.637 kb on + strand at 3415.628 kb on + strand at 3415.629 kb on - strand at 3415.633 kb on - strand at 3415.633 kb on - strand at 3415.644 kb on + strand at 3415.644 kb on + strand at 3415.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,413,781 - -2.1 3,413,879 + -1.4 3,413,880 - -0.8 3,413,880 - -0.8 3,413,880 - +2.1 3,413,905 + +1.0 3,413,906 - +0.7 3,413,906 - -1.6 3,413,906 - -0.7 3,413,906 - -3.2 3,413,906 - +1.0 3,414,067 + -1.6 3,414,067 + +1.0 3,414,067 + -0.8 3,414,068 - +0.0 3,414,136 + -0.8 3,414,202 + Rru_A2964 0.21 -0.8 3,414,202 + Rru_A2964 0.21 +1.4 3,414,202 + Rru_A2964 0.21 -0.8 3,414,202 + Rru_A2964 0.21 -0.4 3,414,203 - Rru_A2964 0.22 +2.0 3,414,203 - Rru_A2964 0.22 -0.6 3,414,203 - Rru_A2964 0.22 -0.9 3,414,203 - Rru_A2964 0.22 -0.3 3,414,256 + Rru_A2964 0.34 -1.2 3,414,361 + Rru_A2964 0.58 -0.8 3,414,361 + Rru_A2964 0.58 -0.6 3,414,361 + Rru_A2964 0.58 -0.3 3,414,361 + Rru_A2964 0.58 +1.9 3,414,361 + Rru_A2964 0.58 -0.6 3,414,361 + Rru_A2964 0.58 -3.8 3,414,361 + Rru_A2964 0.58 -0.3 3,414,361 + Rru_A2964 0.58 -2.4 3,414,361 + Rru_A2964 0.58 -2.8 3,414,361 + Rru_A2964 0.58 +0.6 3,414,361 + Rru_A2964 0.58 +0.4 3,414,362 - Rru_A2964 0.58 -0.6 3,414,362 - Rru_A2964 0.58 +1.0 3,414,362 - Rru_A2964 0.58 +0.5 3,414,362 - Rru_A2964 0.58 +0.8 3,414,362 - Rru_A2964 0.58 -0.3 3,414,362 - Rru_A2964 0.58 -2.5 3,414,362 - Rru_A2964 0.58 +0.2 3,414,362 - Rru_A2964 0.58 -0.5 3,414,637 + +0.8 3,415,628 + +1.5 3,415,629 - -0.3 3,415,633 - +1.0 3,415,633 - -1.3 3,415,644 + +1.0 3,415,644 + -0.4 3,415,644 + -2.4
Or see this region's nucleotide sequence