Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2816 and Rru_A2817 are separated by 151 nucleotides Rru_A2817 and Rru_A2818 are separated by 246 nucleotides Rru_A2818 and Rru_A2819 are separated by 259 nucleotides
Rru_A2816: Rru_A2816 - Transcriptional Regulator, TetR family (NCBI), at 3,247,917 to 3,248,495
_A2816
Rru_A2817: Rru_A2817 - Phasin (NCBI), at 3,248,647 to 3,248,997
_A2817
Rru_A2818: Rru_A2818 - Alanine--tRNA ligase (NCBI), at 3,249,244 to 3,251,895
_A2818
Rru_A2819: Rru_A2819 - RecA DNA recombination protein (NCBI), at 3,252,155 to 3,253,255
_A2819
Position (kb)
3249
3250
3251
3252 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3248.409 kb on - strand, within Rru_A2816 at 3248.493 kb on + strand at 3248.518 kb on + strand at 3248.668 kb on - strand at 3248.888 kb on + strand, within Rru_A2817 at 3248.888 kb on + strand, within Rru_A2817 at 3248.961 kb on + strand, within Rru_A2817 at 3248.962 kb on - strand, within Rru_A2817 at 3249.030 kb on - strand at 3249.030 kb on - strand at 3249.095 kb on + strand at 3249.095 kb on + strand at 3249.096 kb on - strand at 3249.096 kb on - strand at 3249.100 kb on + strand at 3249.100 kb on + strand at 3249.100 kb on + strand at 3249.101 kb on - strand at 3249.101 kb on - strand at 3251.921 kb on - strand at 3251.921 kb on - strand at 3252.043 kb on - strand at 3252.432 kb on + strand, within Rru_A2819
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 3,248,409 - Rru_A2816 0.85 -0.1 3,248,493 + -2.1 3,248,518 + -0.4 3,248,668 - +1.2 3,248,888 + Rru_A2817 0.69 -3.6 3,248,888 + Rru_A2817 0.69 +0.9 3,248,961 + Rru_A2817 0.89 +0.6 3,248,962 - Rru_A2817 0.90 +1.0 3,249,030 - -4.0 3,249,030 - -2.3 3,249,095 + -0.4 3,249,095 + -2.2 3,249,096 - -2.8 3,249,096 - -1.3 3,249,100 + -0.8 3,249,100 + -0.1 3,249,100 + -4.1 3,249,101 - -2.8 3,249,101 - -3.1 3,251,921 - -0.7 3,251,921 - -0.8 3,252,043 - -2.7 3,252,432 + Rru_A2819 0.25 -1.8
Or see this region's nucleotide sequence