Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2574 and Rru_A2575 are separated by 377 nucleotides Rru_A2575 and Rru_A2576 are separated by 129 nucleotides Rru_A2576 and Rru_A2577 are separated by 7 nucleotides
Rru_A2574: Rru_A2574 - Transcriptional Regulator, BadM/Rrf2 family (NCBI), at 2,993,724 to 2,994,188
_A2574
Rru_A2575: Rru_A2575 - hypothetical protein (NCBI), at 2,994,566 to 2,994,973
_A2575
Rru_A2576: Rru_A2576 - 1,4-alpha-glucan branching enzyme (NCBI), at 2,995,103 to 2,997,325
_A2576
Rru_A2577: Rru_A2577 - Glycogen debranching enzyme GlgX (NCBI), at 2,997,333 to 2,999,354
_A2577
Position (kb)
2995
2996
2997
2998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2994.227 kb on - strand at 2994.238 kb on - strand at 2994.268 kb on - strand at 2994.268 kb on - strand at 2994.534 kb on + strand at 2994.535 kb on - strand at 2994.535 kb on - strand at 2994.535 kb on - strand at 2994.537 kb on - strand at 2994.537 kb on - strand at 2994.537 kb on - strand at 2994.537 kb on - strand at 2994.537 kb on - strand at 2994.542 kb on + strand at 2994.542 kb on + strand at 2994.542 kb on + strand at 2994.543 kb on - strand at 2994.543 kb on - strand at 2994.543 kb on - strand at 2994.543 kb on - strand at 2994.543 kb on - strand at 2994.543 kb on - strand at 2994.612 kb on + strand, within Rru_A2575 at 2994.612 kb on + strand, within Rru_A2575 at 2994.612 kb on + strand, within Rru_A2575 at 2994.612 kb on + strand, within Rru_A2575 at 2994.613 kb on - strand, within Rru_A2575 at 2994.613 kb on - strand, within Rru_A2575 at 2994.653 kb on + strand, within Rru_A2575 at 2994.653 kb on + strand, within Rru_A2575 at 2994.654 kb on - strand, within Rru_A2575 at 2994.654 kb on - strand, within Rru_A2575 at 2994.971 kb on + strand at 2994.971 kb on + strand at 2994.971 kb on + strand at 2997.570 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.813 kb on + strand, within Rru_A2577 at 2997.814 kb on - strand, within Rru_A2577 at 2997.814 kb on - strand, within Rru_A2577 at 2997.814 kb on - strand, within Rru_A2577 at 2998.005 kb on + strand, within Rru_A2577 at 2998.005 kb on + strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.006 kb on - strand, within Rru_A2577 at 2998.015 kb on - strand, within Rru_A2577 at 2998.017 kb on - strand, within Rru_A2577 at 2998.050 kb on + strand, within Rru_A2577 at 2998.050 kb on + strand, within Rru_A2577 at 2998.198 kb on - strand, within Rru_A2577 at 2998.198 kb on - strand, within Rru_A2577 at 2998.198 kb on - strand, within Rru_A2577 at 2998.230 kb on + strand, within Rru_A2577
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,994,227 - -2.4 2,994,238 - -1.6 2,994,268 - -0.6 2,994,268 - -1.0 2,994,534 + -2.6 2,994,535 - -1.9 2,994,535 - -1.0 2,994,535 - -2.7 2,994,537 - +0.3 2,994,537 - -1.1 2,994,537 - -3.1 2,994,537 - -2.8 2,994,537 - -0.6 2,994,542 + +0.9 2,994,542 + +1.7 2,994,542 + -3.4 2,994,543 - -2.6 2,994,543 - -0.9 2,994,543 - -2.3 2,994,543 - -1.5 2,994,543 - -0.7 2,994,543 - -1.2 2,994,612 + Rru_A2575 0.11 +0.9 2,994,612 + Rru_A2575 0.11 +0.4 2,994,612 + Rru_A2575 0.11 +1.6 2,994,612 + Rru_A2575 0.11 +0.3 2,994,613 - Rru_A2575 0.12 -1.0 2,994,613 - Rru_A2575 0.12 +1.9 2,994,653 + Rru_A2575 0.21 -0.1 2,994,653 + Rru_A2575 0.21 +3.8 2,994,654 - Rru_A2575 0.22 +0.3 2,994,654 - Rru_A2575 0.22 -0.1 2,994,971 + +0.4 2,994,971 + -0.8 2,994,971 + -0.9 2,997,570 + Rru_A2577 0.12 +0.1 2,997,813 + Rru_A2577 0.24 -3.7 2,997,813 + Rru_A2577 0.24 -2.0 2,997,813 + Rru_A2577 0.24 -0.2 2,997,813 + Rru_A2577 0.24 -1.1 2,997,813 + Rru_A2577 0.24 +0.9 2,997,813 + Rru_A2577 0.24 +1.9 2,997,814 - Rru_A2577 0.24 -0.4 2,997,814 - Rru_A2577 0.24 -2.2 2,997,814 - Rru_A2577 0.24 -2.1 2,998,005 + Rru_A2577 0.33 +0.9 2,998,005 + Rru_A2577 0.33 +1.1 2,998,006 - Rru_A2577 0.33 -0.9 2,998,006 - Rru_A2577 0.33 -2.3 2,998,006 - Rru_A2577 0.33 +0.0 2,998,006 - Rru_A2577 0.33 -0.0 2,998,006 - Rru_A2577 0.33 +0.0 2,998,006 - Rru_A2577 0.33 -0.3 2,998,015 - Rru_A2577 0.34 -0.6 2,998,017 - Rru_A2577 0.34 -0.4 2,998,050 + Rru_A2577 0.35 -0.9 2,998,050 + Rru_A2577 0.35 -2.2 2,998,198 - Rru_A2577 0.43 +1.9 2,998,198 - Rru_A2577 0.43 -0.4 2,998,198 - Rru_A2577 0.43 +1.7 2,998,230 + Rru_A2577 0.44 -0.4
Or see this region's nucleotide sequence