Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2485 and Rru_A2486 overlap by 8 nucleotides Rru_A2486 and Rru_A2487 overlap by 4 nucleotides Rru_A2487 and Rru_A2488 are separated by 2 nucleotides
Rru_A2485: Rru_A2485 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) (NCBI), at 2,888,640 to 2,890,022
_A2485
Rru_A2486: Rru_A2486 - Glucokinase (NCBI), at 2,890,015 to 2,890,989
_A2486
Rru_A2487: Rru_A2487 - BsuBIPstI restriction endonuclease-like (NCBI), at 2,890,986 to 2,892,236
_A2487
Rru_A2488: Rru_A2488 - Putative RNA methylase (NCBI), at 2,892,239 to 2,894,053
_A2488
Position (kb)
2890
2891
2892
2893 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2890.162 kb on + strand, within Rru_A2486 at 2890.162 kb on + strand, within Rru_A2486 at 2890.162 kb on + strand, within Rru_A2486 at 2890.753 kb on + strand, within Rru_A2486 at 2890.831 kb on - strand, within Rru_A2486 at 2890.909 kb on + strand at 2890.910 kb on - strand at 2890.910 kb on - strand at 2890.910 kb on - strand at 2890.987 kb on + strand at 2890.987 kb on + strand at 2890.987 kb on + strand at 2891.132 kb on - strand, within Rru_A2487 at 2891.304 kb on - strand, within Rru_A2487 at 2891.663 kb on - strand, within Rru_A2487 at 2891.828 kb on - strand, within Rru_A2487 at 2891.931 kb on - strand, within Rru_A2487 at 2891.932 kb on + strand, within Rru_A2487 at 2891.932 kb on + strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.977 kb on + strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2892.188 kb on + strand at 2892.188 kb on + strand at 2892.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,890,162 + Rru_A2486 0.15 +0.7 2,890,162 + Rru_A2486 0.15 +0.7 2,890,162 + Rru_A2486 0.15 +1.1 2,890,753 + Rru_A2486 0.76 +1.2 2,890,831 - Rru_A2486 0.84 -0.1 2,890,909 + +0.4 2,890,910 - +0.3 2,890,910 - +0.7 2,890,910 - +0.1 2,890,987 + +0.9 2,890,987 + -1.8 2,890,987 + +1.0 2,891,132 - Rru_A2487 0.12 +0.4 2,891,304 - Rru_A2487 0.25 -0.2 2,891,663 - Rru_A2487 0.54 -0.6 2,891,828 - Rru_A2487 0.67 +1.9 2,891,931 - Rru_A2487 0.76 -2.5 2,891,932 + Rru_A2487 0.76 +0.7 2,891,932 + Rru_A2487 0.76 -2.6 2,891,933 - Rru_A2487 0.76 -0.7 2,891,933 - Rru_A2487 0.76 +0.9 2,891,933 - Rru_A2487 0.76 +1.5 2,891,977 + Rru_A2487 0.79 -1.4 2,891,978 - Rru_A2487 0.79 -0.7 2,891,978 - Rru_A2487 0.79 -4.0 2,891,978 - Rru_A2487 0.79 -0.4 2,892,188 + -0.2 2,892,188 + -2.6 2,892,189 - -0.8
Or see this region's nucleotide sequence