Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2287 and Rru_A2288 are separated by 32 nucleotides Rru_A2288 and Rru_A2289 are separated by 308 nucleotides Rru_A2289 and Rru_A2290 overlap by 1 nucleotides
Rru_A2287: Rru_A2287 - Protein of unknown function DUF86 (NCBI), at 2,646,281 to 2,646,616
_A2287
Rru_A2288: Rru_A2288 - DNA polymerase, beta-like region (NCBI), at 2,646,649 to 2,646,939
_A2288
Rru_A2289: Rru_A2289 - Sulfate adenylyltransferase, small subunit (NCBI), at 2,647,248 to 2,648,138
_A2289
Rru_A2290: Rru_A2290 - Sulfate adenylyltransferase, large subunit (NCBI), at 2,648,138 to 2,650,063
_A2290
Position (kb)
2647
2648
2649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2646.254 kb on + strand at 2646.254 kb on + strand at 2646.255 kb on - strand at 2646.255 kb on - strand at 2646.255 kb on - strand at 2646.547 kb on + strand, within Rru_A2287 at 2646.573 kb on + strand, within Rru_A2287 at 2646.573 kb on + strand, within Rru_A2287 at 2646.573 kb on + strand, within Rru_A2287 at 2646.574 kb on - strand, within Rru_A2287 at 2646.574 kb on - strand, within Rru_A2287 at 2646.912 kb on + strand at 2646.970 kb on + strand at 2647.018 kb on + strand at 2647.019 kb on - strand at 2647.019 kb on - strand at 2647.019 kb on - strand at 2647.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,646,254 + +3.2 2,646,254 + +0.3 2,646,255 - +1.2 2,646,255 - +1.2 2,646,255 - +0.8 2,646,547 + Rru_A2287 0.79 +2.1 2,646,573 + Rru_A2287 0.87 +4.5 2,646,573 + Rru_A2287 0.87 +1.0 2,646,573 + Rru_A2287 0.87 +1.5 2,646,574 - Rru_A2287 0.87 -3.0 2,646,574 - Rru_A2287 0.87 -2.0 2,646,912 + +1.8 2,646,970 + +2.3 2,647,018 + -1.2 2,647,019 - -0.8 2,647,019 - +1.0 2,647,019 - +0.5 2,647,019 - -1.4
Or see this region's nucleotide sequence