Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2021 and Rru_A2022 are separated by 205 nucleotides Rru_A2022 and Rru_A2023 are separated by 451 nucleotides
Rru_A2021: Rru_A2021 - Endonuclease/exonuclease/phosphatase (NCBI), at 2,333,738 to 2,334,478
_A2021
Rru_A2022: Rru_A2022 - hypothetical protein (NCBI), at 2,334,684 to 2,334,896
_A2022
Rru_A2023: Rru_A2023 - chemotaxis sensory transducer (NCBI), at 2,335,348 to 2,337,819
_A2023
Position (kb)
2334
2335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2333.759 kb on - strand at 2333.759 kb on - strand at 2333.814 kb on + strand, within Rru_A2021 at 2333.814 kb on + strand, within Rru_A2021 at 2333.814 kb on + strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2334.097 kb on - strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.219 kb on + strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.257 kb on - strand, within Rru_A2021 at 2334.262 kb on + strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.279 kb on + strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021 at 2334.352 kb on + strand, within Rru_A2021 at 2334.352 kb on + strand, within Rru_A2021 at 2334.352 kb on + strand, within Rru_A2021 at 2334.353 kb on - strand, within Rru_A2021 at 2334.353 kb on - strand, within Rru_A2021 at 2334.424 kb on + strand at 2334.425 kb on - strand at 2334.425 kb on - strand at 2334.537 kb on + strand at 2334.537 kb on + strand at 2334.537 kb on + strand at 2334.538 kb on - strand at 2334.538 kb on - strand at 2334.538 kb on - strand at 2334.545 kb on - strand at 2334.545 kb on - strand at 2334.775 kb on - strand, within Rru_A2022 at 2334.921 kb on + strand at 2334.922 kb on - strand at 2334.922 kb on - strand at 2334.922 kb on - strand at 2334.922 kb on - strand at 2334.922 kb on - strand at 2334.922 kb on - strand at 2334.947 kb on + strand at 2334.947 kb on + strand at 2334.947 kb on + strand at 2334.948 kb on - strand at 2334.948 kb on - strand at 2335.013 kb on + strand at 2335.013 kb on + strand at 2335.074 kb on - strand at 2335.074 kb on - strand at 2335.231 kb on + strand at 2335.231 kb on + strand at 2335.231 kb on + strand at 2335.232 kb on - strand at 2335.232 kb on - strand at 2335.233 kb on + strand at 2335.234 kb on - strand at 2335.353 kb on - strand at 2335.353 kb on - strand at 2335.353 kb on - strand at 2335.442 kb on - strand at 2335.465 kb on + strand at 2335.465 kb on + strand at 2335.465 kb on + strand at 2335.465 kb on + strand at 2335.465 kb on + strand at 2335.466 kb on - strand at 2335.720 kb on + strand, within Rru_A2023 at 2335.721 kb on - strand, within Rru_A2023 at 2335.721 kb on - strand, within Rru_A2023 at 2335.721 kb on - strand, within Rru_A2023
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,333,759 - +1.0 2,333,759 - +1.4 2,333,814 + Rru_A2021 0.10 -2.7 2,333,814 + Rru_A2021 0.10 -1.4 2,333,814 + Rru_A2021 0.10 -0.2 2,333,815 - Rru_A2021 0.10 -2.4 2,333,815 - Rru_A2021 0.10 -3.5 2,333,815 - Rru_A2021 0.10 -2.9 2,334,097 - Rru_A2021 0.48 -0.2 2,334,126 + Rru_A2021 0.52 +4.4 2,334,126 + Rru_A2021 0.52 +0.8 2,334,126 + Rru_A2021 0.52 +1.7 2,334,126 + Rru_A2021 0.52 +1.1 2,334,127 - Rru_A2021 0.52 +1.4 2,334,127 - Rru_A2021 0.52 +1.8 2,334,127 - Rru_A2021 0.52 -1.5 2,334,127 - Rru_A2021 0.52 +0.8 2,334,127 - Rru_A2021 0.52 +0.5 2,334,219 + Rru_A2021 0.65 +2.2 2,334,220 - Rru_A2021 0.65 -1.2 2,334,220 - Rru_A2021 0.65 -4.4 2,334,220 - Rru_A2021 0.65 +0.2 2,334,256 + Rru_A2021 0.70 -2.8 2,334,256 + Rru_A2021 0.70 -1.2 2,334,256 + Rru_A2021 0.70 -1.9 2,334,256 + Rru_A2021 0.70 +0.8 2,334,257 - Rru_A2021 0.70 +0.6 2,334,262 + Rru_A2021 0.71 -1.2 2,334,263 - Rru_A2021 0.71 -2.3 2,334,263 - Rru_A2021 0.71 -0.2 2,334,263 - Rru_A2021 0.71 -0.7 2,334,279 + Rru_A2021 0.73 -0.2 2,334,280 - Rru_A2021 0.73 +0.2 2,334,280 - Rru_A2021 0.73 -2.0 2,334,280 - Rru_A2021 0.73 +0.9 2,334,352 + Rru_A2021 0.83 +0.9 2,334,352 + Rru_A2021 0.83 +1.1 2,334,352 + Rru_A2021 0.83 +0.7 2,334,353 - Rru_A2021 0.83 -0.2 2,334,353 - Rru_A2021 0.83 +4.8 2,334,424 + -3.2 2,334,425 - -0.7 2,334,425 - +0.8 2,334,537 + -1.8 2,334,537 + +1.0 2,334,537 + -3.1 2,334,538 - +0.2 2,334,538 - -0.4 2,334,538 - +0.3 2,334,545 - +0.1 2,334,545 - +1.8 2,334,775 - Rru_A2022 0.43 -2.8 2,334,921 + +3.2 2,334,922 - +0.4 2,334,922 - -1.2 2,334,922 - -2.5 2,334,922 - -1.4 2,334,922 - +0.4 2,334,922 - -0.9 2,334,947 + -1.3 2,334,947 + +1.8 2,334,947 + +4.8 2,334,948 - -0.0 2,334,948 - -1.8 2,335,013 + -4.3 2,335,013 + +0.8 2,335,074 - -2.0 2,335,074 - -0.1 2,335,231 + +0.3 2,335,231 + +1.2 2,335,231 + +1.0 2,335,232 - +1.2 2,335,232 - -0.5 2,335,233 + -0.1 2,335,234 - -0.9 2,335,353 - -1.7 2,335,353 - -1.0 2,335,353 - -2.2 2,335,442 - -2.0 2,335,465 + +2.6 2,335,465 + -0.3 2,335,465 + +0.3 2,335,465 + -0.1 2,335,465 + +0.4 2,335,466 - +0.7 2,335,720 + Rru_A2023 0.15 -0.0 2,335,721 - Rru_A2023 0.15 +0.6 2,335,721 - Rru_A2023 0.15 -0.7 2,335,721 - Rru_A2023 0.15 -1.0
Or see this region's nucleotide sequence