Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1939 and Rru_A1940 overlap by 4 nucleotides Rru_A1940 and Rru_A1941 overlap by 4 nucleotides Rru_A1941 and Rru_A1942 overlap by 19 nucleotides Rru_A1942 and Rru_A1943 are separated by 13 nucleotides
Rru_A1939: Rru_A1939 - Pseudouridine synthase, Rsu (NCBI), at 2,246,881 to 2,247,480
_A1939
Rru_A1940: Rru_A1940 - Pyruvate carboxyltransferase (NCBI), at 2,247,477 to 2,248,391
_A1940
Rru_A1941: Rru_A1941 - acyl-CoA carboxylase alpha chain (NCBI), at 2,248,388 to 2,250,448
_A1941
Rru_A1942: Rru_A1942 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,250,430 to 2,251,257
_A1942
Rru_A1943: Rru_A1943 - Propionyl-CoA carboxylase (NCBI), at 2,251,271 to 2,252,878
_A1943
Position (kb)
2248
2249
2250
2251 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2247.582 kb on + strand, within Rru_A1940 at 2247.582 kb on + strand, within Rru_A1940 at 2247.583 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.621 kb on - strand, within Rru_A1940 at 2247.622 kb on + strand, within Rru_A1940 at 2247.622 kb on + strand, within Rru_A1940 at 2247.622 kb on + strand, within Rru_A1940 at 2247.622 kb on + strand, within Rru_A1940 at 2247.622 kb on + strand, within Rru_A1940 at 2247.623 kb on - strand, within Rru_A1940 at 2247.623 kb on - strand, within Rru_A1940 at 2247.623 kb on - strand, within Rru_A1940 at 2247.757 kb on + strand, within Rru_A1940 at 2247.757 kb on + strand, within Rru_A1940 at 2247.757 kb on + strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.758 kb on - strand, within Rru_A1940 at 2247.944 kb on - strand, within Rru_A1940 at 2247.944 kb on - strand, within Rru_A1940 at 2247.970 kb on + strand, within Rru_A1940 at 2247.970 kb on + strand, within Rru_A1940 at 2247.970 kb on + strand, within Rru_A1940 at 2247.970 kb on + strand, within Rru_A1940 at 2247.970 kb on + strand, within Rru_A1940 at 2247.971 kb on - strand, within Rru_A1940 at 2248.021 kb on - strand, within Rru_A1940 at 2248.100 kb on + strand, within Rru_A1940 at 2248.555 kb on + strand at 2248.555 kb on + strand at 2248.555 kb on + strand at 2248.555 kb on + strand at 2248.555 kb on + strand at 2248.556 kb on - strand at 2248.556 kb on - strand at 2248.556 kb on - strand at 2248.556 kb on - strand at 2248.556 kb on - strand at 2248.717 kb on + strand, within Rru_A1941 at 2248.718 kb on - strand, within Rru_A1941 at 2248.718 kb on - strand, within Rru_A1941 at 2248.718 kb on - strand, within Rru_A1941 at 2248.719 kb on + strand, within Rru_A1941 at 2248.719 kb on - strand, within Rru_A1941 at 2248.720 kb on - strand, within Rru_A1941 at 2248.720 kb on - strand, within Rru_A1941 at 2248.720 kb on - strand, within Rru_A1941 at 2249.304 kb on + strand, within Rru_A1941 at 2249.304 kb on + strand, within Rru_A1941 at 2249.305 kb on - strand, within Rru_A1941 at 2249.592 kb on - strand, within Rru_A1941 at 2249.598 kb on + strand, within Rru_A1941 at 2249.598 kb on + strand, within Rru_A1941 at 2249.599 kb on - strand, within Rru_A1941 at 2249.646 kb on + strand, within Rru_A1941 at 2249.906 kb on + strand, within Rru_A1941 at 2249.906 kb on + strand, within Rru_A1941 at 2249.907 kb on - strand, within Rru_A1941 at 2250.048 kb on + strand, within Rru_A1941 at 2250.049 kb on - strand, within Rru_A1941 at 2250.049 kb on - strand, within Rru_A1941 at 2250.270 kb on + strand at 2250.815 kb on + strand, within Rru_A1942 at 2250.815 kb on + strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.402 kb on - strand at 2251.404 kb on + strand at 2251.405 kb on - strand at 2251.405 kb on - strand at 2251.425 kb on + strand at 2251.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,247,582 + Rru_A1940 0.11 -0.2 2,247,582 + Rru_A1940 0.11 +0.6 2,247,583 - Rru_A1940 0.12 -1.1 2,247,621 - Rru_A1940 0.16 -0.5 2,247,621 - Rru_A1940 0.16 -1.9 2,247,621 - Rru_A1940 0.16 -1.3 2,247,621 - Rru_A1940 0.16 -2.2 2,247,621 - Rru_A1940 0.16 -0.0 2,247,621 - Rru_A1940 0.16 -2.3 2,247,621 - Rru_A1940 0.16 +0.7 2,247,622 + Rru_A1940 0.16 +0.8 2,247,622 + Rru_A1940 0.16 -1.4 2,247,622 + Rru_A1940 0.16 -1.2 2,247,622 + Rru_A1940 0.16 +0.7 2,247,622 + Rru_A1940 0.16 -0.8 2,247,623 - Rru_A1940 0.16 +0.1 2,247,623 - Rru_A1940 0.16 -1.2 2,247,623 - Rru_A1940 0.16 -0.4 2,247,757 + Rru_A1940 0.31 -1.7 2,247,757 + Rru_A1940 0.31 -1.2 2,247,757 + Rru_A1940 0.31 -0.8 2,247,758 - Rru_A1940 0.31 +1.3 2,247,758 - Rru_A1940 0.31 -0.6 2,247,758 - Rru_A1940 0.31 +1.2 2,247,758 - Rru_A1940 0.31 -0.2 2,247,758 - Rru_A1940 0.31 +0.2 2,247,758 - Rru_A1940 0.31 -1.4 2,247,944 - Rru_A1940 0.51 +0.2 2,247,944 - Rru_A1940 0.51 +1.8 2,247,970 + Rru_A1940 0.54 +0.0 2,247,970 + Rru_A1940 0.54 -2.2 2,247,970 + Rru_A1940 0.54 -1.6 2,247,970 + Rru_A1940 0.54 -0.1 2,247,970 + Rru_A1940 0.54 -2.2 2,247,971 - Rru_A1940 0.54 -2.5 2,248,021 - Rru_A1940 0.59 -1.0 2,248,100 + Rru_A1940 0.68 +1.5 2,248,555 + -2.0 2,248,555 + +0.1 2,248,555 + +0.2 2,248,555 + +1.8 2,248,555 + -2.7 2,248,556 - -2.1 2,248,556 - -0.8 2,248,556 - -2.9 2,248,556 - +1.5 2,248,556 - -0.5 2,248,717 + Rru_A1941 0.16 +1.8 2,248,718 - Rru_A1941 0.16 -1.1 2,248,718 - Rru_A1941 0.16 -0.2 2,248,718 - Rru_A1941 0.16 +1.0 2,248,719 + Rru_A1941 0.16 -0.5 2,248,719 - Rru_A1941 0.16 -1.2 2,248,720 - Rru_A1941 0.16 -1.4 2,248,720 - Rru_A1941 0.16 -0.7 2,248,720 - Rru_A1941 0.16 -2.1 2,249,304 + Rru_A1941 0.44 +0.7 2,249,304 + Rru_A1941 0.44 -0.5 2,249,305 - Rru_A1941 0.44 -0.5 2,249,592 - Rru_A1941 0.58 +3.4 2,249,598 + Rru_A1941 0.59 -0.5 2,249,598 + Rru_A1941 0.59 -1.8 2,249,599 - Rru_A1941 0.59 +0.2 2,249,646 + Rru_A1941 0.61 -0.5 2,249,906 + Rru_A1941 0.74 -0.2 2,249,906 + Rru_A1941 0.74 -2.1 2,249,907 - Rru_A1941 0.74 -1.5 2,250,048 + Rru_A1941 0.81 +0.5 2,250,049 - Rru_A1941 0.81 +1.6 2,250,049 - Rru_A1941 0.81 -0.5 2,250,270 + +1.3 2,250,815 + Rru_A1942 0.46 -2.6 2,250,815 + Rru_A1942 0.46 +0.9 2,250,816 - Rru_A1942 0.47 +1.1 2,250,816 - Rru_A1942 0.47 +0.5 2,250,816 - Rru_A1942 0.47 -0.2 2,250,816 - Rru_A1942 0.47 +0.8 2,250,816 - Rru_A1942 0.47 -0.3 2,250,816 - Rru_A1942 0.47 -1.0 2,250,929 + Rru_A1942 0.60 +2.8 2,250,929 + Rru_A1942 0.60 -0.8 2,251,401 + -1.2 2,251,401 + -1.4 2,251,401 + +3.1 2,251,401 + +1.8 2,251,402 - -1.7 2,251,404 + +1.0 2,251,405 - -2.0 2,251,405 - -3.8 2,251,425 + +2.9 2,251,426 - -0.8
Or see this region's nucleotide sequence