Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1848 and Rru_A1849 are separated by 36 nucleotides Rru_A1849 and Rru_A1850 are separated by 20 nucleotides Rru_A1850 and Rru_A1851 are separated by 12 nucleotides Rru_A1851 and Rru_A1852 are separated by 212 nucleotides Rru_A1852 and Rru_A1853 are separated by 93 nucleotides
Rru_A1848: Rru_A1848 - Gram negative topoisomerase IV, subunit A (NCBI), at 2,146,560 to 2,148,791
_A1848
Rru_A1849: Rru_A1849 - Recombination protein O, RecO (NCBI), at 2,148,828 to 2,149,586
_A1849
Rru_A1850: Rru_A1850 - hypothetical protein (NCBI), at 2,149,607 to 2,149,777
_A1850
Rru_A1851: Rru_A1851 - GTP-binding protein Era (NCBI), at 2,149,790 to 2,150,719
_A1851
Rru_A1852: Rru_A1852 - Ribonuclease III (NCBI), at 2,150,932 to 2,151,597
_A1852
Rru_A1853: Rru_A1853 - Peptidase S26A, signal peptidase I (NCBI), at 2,151,691 to 2,152,428
_A1853
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2148.823 kb on - strand at 2148.823 kb on - strand at 2148.823 kb on - strand at 2148.823 kb on - strand at 2148.823 kb on - strand at 2148.932 kb on - strand, within Rru_A1849 at 2149.081 kb on + strand, within Rru_A1849 at 2149.081 kb on + strand, within Rru_A1849 at 2149.250 kb on - strand, within Rru_A1849 at 2149.609 kb on - strand at 2150.399 kb on - strand, within Rru_A1851 at 2150.399 kb on - strand, within Rru_A1851 at 2150.870 kb on + strand at 2150.870 kb on + strand at 2150.871 kb on - strand at 2150.871 kb on - strand at 2151.428 kb on + strand, within Rru_A1852 at 2151.645 kb on - strand at 2151.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,148,823 - +0.1 2,148,823 - +1.1 2,148,823 - -2.6 2,148,823 - -1.5 2,148,823 - -1.0 2,148,932 - Rru_A1849 0.14 -0.3 2,149,081 + Rru_A1849 0.33 +0.8 2,149,081 + Rru_A1849 0.33 +1.2 2,149,250 - Rru_A1849 0.56 -1.3 2,149,609 - +0.1 2,150,399 - Rru_A1851 0.65 +1.8 2,150,399 - Rru_A1851 0.65 +0.8 2,150,870 + -0.2 2,150,870 + -0.5 2,150,871 - +1.8 2,150,871 - -2.4 2,151,428 + Rru_A1852 0.74 +1.8 2,151,645 - +0.8 2,151,645 - -2.0
Or see this region's nucleotide sequence