Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1772 and Rru_A1773 are separated by 76 nucleotides Rru_A1773 and Rru_A1774 are separated by 273 nucleotides Rru_A1774 and Rru_A1775 overlap by 4 nucleotides Rru_A1775 and Rru_A1776 are separated by 20 nucleotides
Rru_A1772: Rru_A1772 - Twin-arginine translocation protein TatB (NCBI), at 2,064,664 to 2,065,116
_A1772
Rru_A1773: Rru_A1773 - Twin-arginine translocation protein TatA/E (NCBI), at 2,065,193 to 2,065,483
_A1773
Rru_A1774: Rru_A1774 - Predicted transcriptional regulator (NCBI), at 2,065,757 to 2,066,476
_A1774
Rru_A1775: Rru_A1775 - chromosome segregation and condensation protein ScpA (NCBI), at 2,066,473 to 2,067,285
_A1775
Rru_A1776: Rru_A1776 - Peptidase M50 (NCBI), at 2,067,306 to 2,067,992
_A1776
Position (kb)
2065
2066
2067 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2065.563 kb on - strand at 2065.758 kb on + strand at 2065.865 kb on - strand, within Rru_A1774 at 2066.726 kb on + strand, within Rru_A1775 at 2066.726 kb on + strand, within Rru_A1775 at 2066.727 kb on - strand, within Rru_A1775 at 2066.808 kb on - strand, within Rru_A1775 at 2066.808 kb on - strand, within Rru_A1775 at 2067.110 kb on + strand, within Rru_A1775 at 2067.110 kb on + strand, within Rru_A1775 at 2067.334 kb on + strand at 2067.335 kb on - strand at 2067.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,065,563 - -2.1 2,065,758 + -0.5 2,065,865 - Rru_A1774 0.15 +0.2 2,066,726 + Rru_A1775 0.31 -1.7 2,066,726 + Rru_A1775 0.31 +1.8 2,066,727 - Rru_A1775 0.31 -0.2 2,066,808 - Rru_A1775 0.41 +0.8 2,066,808 - Rru_A1775 0.41 -0.6 2,067,110 + Rru_A1775 0.78 +2.1 2,067,110 + Rru_A1775 0.78 -0.6 2,067,334 + -1.7 2,067,335 - -1.6 2,067,335 - +2.2
Or see this region's nucleotide sequence