Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1742 and Rru_A1743 are separated by 96 nucleotides Rru_A1743 and Rru_A1744 overlap by 14 nucleotides Rru_A1744 and Rru_A1745 are separated by 305 nucleotides
Rru_A1742: Rru_A1742 - Peptidylprolyl isomerase (NCBI), at 2,033,249 to 2,033,755
_A1742
Rru_A1743: Rru_A1743 - Coenzyme A biosynthesis protein (NCBI), at 2,033,852 to 2,034,370
_A1743
Rru_A1744: Rru_A1744 - DNA gyrase, subunit A (NCBI), at 2,034,357 to 2,037,188
_A1744
Rru_A1745: Rru_A1745 - Single-strand binding protein (NCBI), at 2,037,494 to 2,038,051
_A1745
Position (kb)
2034
2035
2036
2037
2038 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2033.382 kb on + strand, within Rru_A1742 at 2033.382 kb on + strand, within Rru_A1742 at 2033.382 kb on + strand, within Rru_A1742 at 2033.383 kb on - strand, within Rru_A1742 at 2033.383 kb on - strand, within Rru_A1742 at 2033.383 kb on - strand, within Rru_A1742 at 2033.715 kb on + strand at 2033.716 kb on - strand at 2033.716 kb on - strand at 2033.716 kb on - strand at 2033.778 kb on - strand at 2033.787 kb on - strand at 2033.787 kb on - strand at 2033.787 kb on - strand at 2033.809 kb on + strand at 2033.810 kb on - strand at 2037.495 kb on + strand at 2037.496 kb on - strand at 2038.146 kb on - strand at 2038.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,033,382 + Rru_A1742 0.26 -0.6 2,033,382 + Rru_A1742 0.26 +0.4 2,033,382 + Rru_A1742 0.26 -1.7 2,033,383 - Rru_A1742 0.26 -0.2 2,033,383 - Rru_A1742 0.26 -1.7 2,033,383 - Rru_A1742 0.26 -0.2 2,033,715 + -1.0 2,033,716 - -0.8 2,033,716 - -2.9 2,033,716 - -2.6 2,033,778 - -2.1 2,033,787 - -0.8 2,033,787 - +1.2 2,033,787 - -2.1 2,033,809 + +0.2 2,033,810 - -1.2 2,037,495 + -2.2 2,037,496 - +0.3 2,038,146 - +1.8 2,038,146 - -1.6
Or see this region's nucleotide sequence